CAJ | 학술논문

分析了灰斑古毒蛾核型多角体病毒(Orgyia ericae nucleopolyhedrovirus,OrerNPV)EcoR I-O 片段分子生物学特征,为研究egt基因分子特性和杆状病毒遗传改良提供科学依据.克隆和分析了OrerNPV EcoR I-O 片段所包含的2个基因,并应用生物软件对OrerNPV蜕皮甾体尿苷二磷酸葡糖转移酶(egt)基因及其启动子进行了比对分析.结果表明OrerNPV EcoR I-O片段包含egt基因和Ol-129同源基因,在该区域中编码egt基因的开放阅读框(ORF)由1 539个核苷酸组成,可以编码512个氨基酸,预计蛋白质分子质量为57.9 kDa.OrerNPV和NPVs之间的同源性为46%～89%,与白斑天幕毛虫核型多角体病毒(Orgyia leucostigma NPV,OlNPV)egt基因同源性最高,达89%,与棉褐带卷叶蛾核型多角体病毒(Adoxophyes orana NPV,AoNPV)同源性最低为46%;而OrerNPV和 GVs 之间的同源性为 44%～55%,小于与NPVs之间的同源性;与昆虫UGT间同源性最低,与家蚕UGT间同源性为41%.EcoR I-O片段+M13端含有编码OlNPV ORF129(Ol-129)Ol-129同源基因ORF,由597 bp个核苷酸组成,在其起始密码子ATG上游-64,-24 nt处各有1个TATA box,其推测的氨基酸序列与Ol-129同源性最高达81%,与LdNPV ORF127同源性最低,为40%.明确了OrerNPV EcoR I-O 片段的序列特征,以及OrerNPV egt 基因特性.
분석료회반고독아핵형다각체병독(Orgyia ericae nucleopolyhedrovirus,OrerNPV)EcoR I-O 편단분자생물학특정,위연구egt기인분자특성화간상병독유전개량제공과학의거.극륭화분석료OrerNPV EcoR I-O 편단소포함적2개기인,병응용생물연건대OrerNPV세피치체뇨감이린산포당전이매(egt)기인급기계동자진행료비대분석.결과표명OrerNPV EcoR I-O편단포함egt기인화Ol-129동원기인,재해구역중편마egt기인적개방열독광(ORF)유1 539개핵감산조성,가이편마512개안기산,예계단백질분자질량위57.9 kDa.OrerNPV화NPVs지간적동원성위46%～89%,여백반천막모충핵형다각체병독(Orgyia leucostigma NPV,OlNPV)egt기인동원성최고,체89%,여면갈대권협아핵형다각체병독(Adoxophyes orana NPV,AoNPV)동원성최저위46%;이OrerNPV화 GVs 지간적동원성위 44%～55%,소우여NPVs지간적동원성;여곤충UGT간동원성최저,여가잠UGT간동원성위41%.EcoR I-O편단+M13단함유편마OlNPV ORF129(Ol-129)Ol-129동원기인ORF,유597 bp개핵감산조성,재기기시밀마자ATG상유-64,-24 nt처각유1개TATA box,기추측적안기산서렬여Ol-129동원성최고체81%,여LdNPV ORF127동원성최저,위40%.명학료OrerNPV EcoR I-O 편단적서렬특정,이급OrerNPV egt 기인특성.
It is not specified of the molecular characterization on Orgyia ericae Nucleopolyhedrovirus(OrerNPV).Here,the cloned OrerNPV EcoR I-O fragment was analyzed,which the egt gene and homologues of Ol-129 gene were found in this fragment.The OrerNPV egt open reading frame was 1 539 nucleotides long that coded a peptide of 512 amino acids with a predicted molecular weight of 57.9 kDa.The peptide of OrerNPV egt shared a highest similarity of 89% to Orgyia leucostigma nucleopolyhedrovirus;while it shared the lowest similarity with Adoxophyes orana nucleopolyhedrovirus 46%.The homologues of Ol-129 gene amino acid residues in the M13+ terminal of OrerNPV had 89%- 40% identities with homologous genes from other 10 NPVs.The result showed that characteristic of OrerNPV EcoR I-O fragment and OrerNPV egt gene.