CAJ | 학술논문

从湖北红安发病花生根部分离到一个细菌分离物2C1,对该分离物进行了TZC显色反应,致病力的测定,16S rRNA、23S rRNA和鞭毛蛋白基因filC的PCR扩增,16S rRNA PCR扩增产物的序列分析.结果显示,分离物2C1在TZC培养基上表现出青枯菌的典型菌落形态,对花生具有强致病性,PCR扩增获得与预期大小的产物片段; 16S rRNA核苷酸序列具有与其它青枯菌分离物99%左右的一致性.上述结果表明:2C1是具有强致病力的花生青枯菌分离物.
종호북홍안발병화생근부분리도일개세균분리물2C1,대해분리물진행료TZC현색반응,치병력적측정,16S rRNA、23S rRNA화편모단백기인filC적PCR확증,16S rRNA PCR확증산물적서렬분석.결과현시,분리물2C1재TZC배양기상표현출청고균적전형균락형태,대화생구유강치병성,PCR확증획득여예기대소적산물편단; 16S rRNA핵감산서렬구유여기타청고균분리물99%좌우적일치성.상술결과표명:2C1시구유강치병력적화생청고균분리물.
A bacterium isolate 2C1 from the root of diseased peanut seedling from Hubei Hongan was identified by its pathogenecity to peanut,PCR amplification of 16S rRNA,23S rRNA and filC gene and 16S rRNA PCR product sequencing.The results revealed that 2C1 was virulent to peanut and showed the typical bacterial wilt symptom, and it amplified the prospected size of fragments of 16S rRNA,23S rRNA and filC gene of Ralstonia solanacearum by PCR,and its 16S rRNA PCR product showed 99% nucleotide matching with those strains of R.solanacearum.