CAJ | 학술논문

目的了解河南省狂犬病毒与人用、兽用狂犬病疫苗株在糖蛋白基因核苷酸和氨基酸序列水平上的差异,为有效控制狂犬病疫情提供初步的科学依据.方法以反转录-半套式聚合酶链反应(RT-heminested-PCR)扩增2006年12月分离自河南省信阳市的9株狂犬病毒街毒株,经纯化、克隆、测序后获得9条糖蛋白基因全长序列,采用生物信息学软件构建基因系统发育树,对糖蛋白基因序列和氨基酸序列进行分析.结果 9株狂犬病毒糖蛋白在核苷酸及氨基酸序列水平上彼此的同源性分别为97.6%～98.9%和99.2%～99.8%;9株病毒与CTN疫苗的同源性最高,其核苷酸及氨基酸同源性分别为85.6%～93.0%和91.9%～92.9%;9株病毒与其他疫苗株相比,其核苷酸及氨基酸同源性分别为80.4%～83.3%和87.7%～92.5%;与已知的基因1型狂犬病毒比较,9株病毒糖蛋白氨基酸序列发生了若干位点的氨基酸取代.结论 9株河南省狂犬病毒街毒株均属基因1型,CTN疫苗株可能为目前我国河南省所流行的狂犬病提供较好的保护效果.
목적 료해하남성광견병독여인용、수용광견병역묘주재당단백기인핵감산화안기산서렬수평상적차이,위유효공제광견병역정제공초보적과학의거.방법 이반전록-반투식취합매련반응(RT-heminested-PCR)확증2006년12월분리자하남성신양시적9주광견병독가독주,경순화、극륭、측서후획득9조당단백기인전장서렬,채용생물신식학연건구건기인계통발육수,대당단백기인서렬화안기산서렬진행분석.결과 9주광견병독당단백재핵감산급안기산서렬수평상피차적동원성분별위97.6%～98.9%화99.2%～99.8%;9주병독여CTN역묘적동원성최고,기핵감산급안기산동원성분별위85.6%～93.0%화91.9%～92.9%;9주병독여기타역묘주상비,기핵감산급안기산동원성분별위80.4%～83.3%화87.7%～92.5%;여이지적기인1형광견병독비교,9주병독당단백안기산서렬발생료약간위점적안기산취대.결론 9주하남성광견병독가독주균속기인1형,CTN역묘주가능위목전아국하남성소류행적광견병제공교호적보호효과.
Objective To understand the nucleotide and amino acid differences of glycoprotein gene (G gene) between isolated rabies viruses in Henan Province and rabies vaccine strains used for human and animals. Methods G gene sequences of nine rabies viruses isolated from dogs in Xinyang city of Henan Province in December 2006 were amplified by reverse transcriptase (RT)-heminestedpolymerase chain reaction (PCR), and then were cloned and sequenced. The phylogenetic trees were constructed for analyzing the genetic characteristics of these rabies viruses. Results The homology of G gene among the nine isolates from Henan Province was 97.6% - 98.9% at nucleotide level and 99.2%-99.8% at amino acid level. The similarities between these isolates and CTN vaccine strain were 85.6%-93.0% and 91.9%-92.9% at nucleotide and amino acid level, respectively, which were higher than those between these isolates and other vaccine strains (80.4% - 83.3% and 87.7% - 92.5% at nucleotide and amino acid level, respectively). The nine isolates had several amino acid substitutions when compared to other genotype 1 rabies virus strains. Conclusions The nine rabies viruses strains isolated from Henan Province all belong to genotype 1. CTN may be an effective vaccine for preventing rabies in Henan Province.