中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2011年
3期
206-209
,共4页
贾强%只达石%黄慧玲%蔡英%武俏丽%张学斌%常小丽
賈彊%隻達石%黃慧玲%蔡英%武俏麗%張學斌%常小麗
가강%지체석%황혜령%채영%무초려%장학빈%상소려
脑损伤%促红细胞生成素%受体
腦損傷%促紅細胞生成素%受體
뇌손상%촉홍세포생성소%수체
Brain injuries%Erythropoietin%Receptor
目的 研究创伤性颅脑损伤(TBI)大鼠损伤周围脑组织促红细胞生成素(erythropoietin,EPO)及其受体(erythropoietin receptor,EPOR)的表达.方法 78只SD大鼠按随机数字表法分为对照组(6只)、假手术组(36只)、液压冲击脑创伤模型组(36只).根据处死时间,分为6,24 h和3,5,7,14 d6个时相点,每个时相点假手术组和脑损伤组各处死6只大鼠,取损伤周围脑组织.利用实时荧光定量PCR和Western blot方法检测EPO、EPOR的mRNA表达和蛋白表达.结果 EPO在伤后24 h内即升高,到第3天达到高峰,并可维持2 d左右,至伤后第7天开始下降,于伤后14 d基本恢复至伤前水平;而EPOR于伤后24 h到达高峰,至伤后14 d表达量仍可维持较高的水平.结论 TBI后24 h内源性EPO及其受体的表达即开始增加,但二者的表达具有不一致性,且EPO相对受体表达具有短暂性.
目的 研究創傷性顱腦損傷(TBI)大鼠損傷週圍腦組織促紅細胞生成素(erythropoietin,EPO)及其受體(erythropoietin receptor,EPOR)的錶達.方法 78隻SD大鼠按隨機數字錶法分為對照組(6隻)、假手術組(36隻)、液壓遲擊腦創傷模型組(36隻).根據處死時間,分為6,24 h和3,5,7,14 d6箇時相點,每箇時相點假手術組和腦損傷組各處死6隻大鼠,取損傷週圍腦組織.利用實時熒光定量PCR和Western blot方法檢測EPO、EPOR的mRNA錶達和蛋白錶達.結果 EPO在傷後24 h內即升高,到第3天達到高峰,併可維持2 d左右,至傷後第7天開始下降,于傷後14 d基本恢複至傷前水平;而EPOR于傷後24 h到達高峰,至傷後14 d錶達量仍可維持較高的水平.結論 TBI後24 h內源性EPO及其受體的錶達即開始增加,但二者的錶達具有不一緻性,且EPO相對受體錶達具有短暫性.
목적 연구창상성로뇌손상(TBI)대서손상주위뇌조직촉홍세포생성소(erythropoietin,EPO)급기수체(erythropoietin receptor,EPOR)적표체.방법 78지SD대서안수궤수자표법분위대조조(6지)、가수술조(36지)、액압충격뇌창상모형조(36지).근거처사시간,분위6,24 h화3,5,7,14 d6개시상점,매개시상점가수술조화뇌손상조각처사6지대서,취손상주위뇌조직.이용실시형광정량PCR화Western blot방법검측EPO、EPOR적mRNA표체화단백표체.결과 EPO재상후24 h내즉승고,도제3천체도고봉,병가유지2 d좌우,지상후제7천개시하강,우상후14 d기본회복지상전수평;이EPOR우상후24 h도체고봉,지상후14 d표체량잉가유지교고적수평.결론 TBI후24 h내원성EPO급기수체적표체즉개시증가,단이자적표체구유불일치성,차EPO상대수체표체구유단잠성.
Objective To study the expressions of erythmpoietin(EPO)and its receptors(EPOR)in the injured brain tissue ofthe rats with traumatic brain injury(TBI).Methods A total of78 SD rats were randomly divided into three groups including control group(six rats),sham group(36rats) and fluid percussion injury group(36 rats).The rats were sacrificed at 6,24 hours,3,5,7 and 14days after TBI in the sham group and the fluid percussion injury group(six rats at each time point).Then,the injured brain tissues were removed for observation of the mRNA and protein expressions of EPO and EPOR by meaDiB of real-time PCR and Western blot. Results The expression of EPO was increased at 24 hours and reached the peak at day 3 after TBI.The hish expression level of EPO could maintain for two days or so.began to decrease at day 7 and recovered to normal at day 14 after Till.While the expression of EPOR reached the peak at 24 hours after TBI and maintained hish level at day14. Conclusions The expressions of EPO and EPOR show increase within 24 hours after TBI.In fact,the expressions of both factors are not in consistency,with more transient expression of EPO.