中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2012年
8期
638-640
,共3页
柳迎昭%朱晨露%田洁%陈建华%陈建国%陈娟%许化溪%王胜军
柳迎昭%硃晨露%田潔%陳建華%陳建國%陳娟%許化溪%王勝軍
류영소%주신로%전길%진건화%진건국%진연%허화계%왕성군
桥本甲状腺炎%Th17细胞%自身免疫%甲状腺
橋本甲狀腺炎%Th17細胞%自身免疫%甲狀腺
교본갑상선염%Th17세포%자신면역%갑상선
Hashimoto's thyroiditis%Th17 cells%Autoimmune diseases%Thyroid gland
目的 研究桥本甲状腺炎(HT)患者Th17细胞及其相关因子的变化.方法 选取30例桥本甲状腺炎患者,并以30名健康对照进行研究.Th17细胞采用流式细胞术测定,孤儿核受体γt( RORγt)和白细胞介素(IL)-17采用实时定量PCR测定,IL-6和IL-23采用ELISA法测定.结果 (1)与健康对照相比,桥本甲状腺炎患者外周血中Th17细胞比例明显增加[(0.75±0.79)％对(0.28±0.23)％],RORγt水平增高(0.30±0.38对0.04±0.02,均P＜0.05)；(2)桥本甲状腺炎患者血清中IL-6和IL-23水平显著高于健康对照组(3.66±4.70对0.47±1.11,154.7±75.81对80.65 ±61.41,均P＜0.05)；(3)桥本甲状腺炎患者Th17细胞与甲状腺球蛋白抗体(TgAb)水平呈正相关(r=0.848 4,P=0.0077);(4)桥本甲状腺炎患者甲状腺组织中表达RORγt和IL-17基因.结论 桥本甲状腺炎患者体内Th17细胞及相关因子异常增高.
目的 研究橋本甲狀腺炎(HT)患者Th17細胞及其相關因子的變化.方法 選取30例橋本甲狀腺炎患者,併以30名健康對照進行研究.Th17細胞採用流式細胞術測定,孤兒覈受體γt( RORγt)和白細胞介素(IL)-17採用實時定量PCR測定,IL-6和IL-23採用ELISA法測定.結果 (1)與健康對照相比,橋本甲狀腺炎患者外週血中Th17細胞比例明顯增加[(0.75±0.79)％對(0.28±0.23)％],RORγt水平增高(0.30±0.38對0.04±0.02,均P＜0.05)；(2)橋本甲狀腺炎患者血清中IL-6和IL-23水平顯著高于健康對照組(3.66±4.70對0.47±1.11,154.7±75.81對80.65 ±61.41,均P＜0.05)；(3)橋本甲狀腺炎患者Th17細胞與甲狀腺毬蛋白抗體(TgAb)水平呈正相關(r=0.848 4,P=0.0077);(4)橋本甲狀腺炎患者甲狀腺組織中錶達RORγt和IL-17基因.結論 橋本甲狀腺炎患者體內Th17細胞及相關因子異常增高.
목적 연구교본갑상선염(HT)환자Th17세포급기상관인자적변화.방법 선취30례교본갑상선염환자,병이30명건강대조진행연구.Th17세포채용류식세포술측정,고인핵수체γt( RORγt)화백세포개소(IL)-17채용실시정량PCR측정,IL-6화IL-23채용ELISA법측정.결과 (1)여건강대조상비,교본갑상선염환자외주혈중Th17세포비례명현증가[(0.75±0.79)％대(0.28±0.23)％],RORγt수평증고(0.30±0.38대0.04±0.02,균P＜0.05)；(2)교본갑상선염환자혈청중IL-6화IL-23수평현저고우건강대조조(3.66±4.70대0.47±1.11,154.7±75.81대80.65 ±61.41,균P＜0.05)；(3)교본갑상선염환자Th17세포여갑상선구단백항체(TgAb)수평정정상관(r=0.848 4,P=0.0077);(4)교본갑상선염환자갑상선조직중표체RORγt화IL-17기인.결론 교본갑상선염환자체내Th17세포급상관인자이상증고.
Objective To study the alteration of Th17 cells and related molecules in patients with Hashimoto's thyroiditis (HT).Methods Th17 cells were determined by flow cytometry.Real-time PCR method was applied to detect the expression of the orphan nuclear receptor RORγt and interleukin(IL) -17.Serum IL-6 and IL-23 were detected by ELISA method.Results ( 1 ) Compared with healthy controls,the frequency of Th17 cells [ (0.75± 0.79) ％ vs ( 0.28 ± 0.23 )％ ] and expression of RORγt ( 0.30 ± 0.38 vs 0.04 ± 0.02,both P ＜ 0.05 ) were significantly increased in HT patients.( 2 ) The levels of IL-6 and IL23 in HT patients were higher than those in healthy controls ( 3.66 ± 4.70 vs 0.47 ± 1.11,154.7 ± 75.81 vs 80.65 ± 61.41,both P＜0.05 ).( 3 ) A positive correlation between Th17 cells and serum TgAb was revealed in HT patients ( r =0.848 4,P =0.007 7 ).(4) The results of PCR showed that IL-17 and RORγt expressed in thyroid tissues of patients HT.Conclusion An increased frequency of Thl7 cells was found in HT patients,implying that this cell subset may play an important role in the pathogenesis of autoimmune thyroid disease.
