中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
3期
372-374
,共3页
二异丙酚%内毒素血症%内皮细胞%肾小球%毛细血管通透性
二異丙酚%內毒素血癥%內皮細胞%腎小毬%毛細血管通透性
이이병분%내독소혈증%내피세포%신소구%모세혈관통투성
Propofol%Endotoxemia%Endothelial cells%Kidney glomerulus%Capillary permeability
目的 探讨异丙酚预先给药对脂多糖(LPS)诱导大鼠肾小球血管内皮细胞通透性升高的影响.方法 分离、培养SD大鼠肾小球血管内皮细胞,以1×106/ml的密度接种于24孔培养板(200 μl/孔)和transwell小室(100 μl/室),采用随机数字表法,将其随机分为6组(n=10),正常对照组(C组)不作任何处理;脂肪乳对照组(I 组)加入10%脂肪乳4 μg/ml;异丙酚组(P组)加入异丙酚4μg/ml;LPS组(L组):加入LPS 10μg/ml;LPS+脂肪乳组(L+I组)加入10%脂肪乳4 μg/ml及LPS 10μg/ml;LPS+异丙酚组(L+P组)加入异丙酚4μg/ml 及LPS 10 μg/ml.于加入LPS前30 min加入脂肪乳或异丙酚,药物的浓度均为终浓度.加入LPS后6 h,收集细胞,采用逆转录-聚合酶链反应测定血管内皮细胞生长因子(VEGF)mRNA表达水平;收集上清液,采用酶联免疫吸附法测定VEGF浓度;测定血管内皮细胞通透性.结果 与C组比较,L组、L+I组和L+P组VEGF mRNA表达上调,上清液中VEGF浓度和血管内皮细胞通透性增加(P<0.05),而I组和P组上述指标差异无统计学意义(P>O.05).与L组比较,L+P组VEGF mRNA表达下凋,上清液中VEGF浓度和血管内皮细胞通透性降低(P<0.05),L+I组上述指标差异无统计学意义(P>0.05).上清液中VEGF浓度与血管内皮细胞通透性呈正相关(r=0.833,P<0.05).结论 异丙酚预先给药可抑制LPS诱导大鼠肾小球血管内皮细胞通透性升高,其机制与下调VEGF表达有关.
目的 探討異丙酚預先給藥對脂多糖(LPS)誘導大鼠腎小毬血管內皮細胞通透性升高的影響.方法 分離、培養SD大鼠腎小毬血管內皮細胞,以1×106/ml的密度接種于24孔培養闆(200 μl/孔)和transwell小室(100 μl/室),採用隨機數字錶法,將其隨機分為6組(n=10),正常對照組(C組)不作任何處理;脂肪乳對照組(I 組)加入10%脂肪乳4 μg/ml;異丙酚組(P組)加入異丙酚4μg/ml;LPS組(L組):加入LPS 10μg/ml;LPS+脂肪乳組(L+I組)加入10%脂肪乳4 μg/ml及LPS 10μg/ml;LPS+異丙酚組(L+P組)加入異丙酚4μg/ml 及LPS 10 μg/ml.于加入LPS前30 min加入脂肪乳或異丙酚,藥物的濃度均為終濃度.加入LPS後6 h,收集細胞,採用逆轉錄-聚閤酶鏈反應測定血管內皮細胞生長因子(VEGF)mRNA錶達水平;收集上清液,採用酶聯免疫吸附法測定VEGF濃度;測定血管內皮細胞通透性.結果 與C組比較,L組、L+I組和L+P組VEGF mRNA錶達上調,上清液中VEGF濃度和血管內皮細胞通透性增加(P<0.05),而I組和P組上述指標差異無統計學意義(P>O.05).與L組比較,L+P組VEGF mRNA錶達下凋,上清液中VEGF濃度和血管內皮細胞通透性降低(P<0.05),L+I組上述指標差異無統計學意義(P>0.05).上清液中VEGF濃度與血管內皮細胞通透性呈正相關(r=0.833,P<0.05).結論 異丙酚預先給藥可抑製LPS誘導大鼠腎小毬血管內皮細胞通透性升高,其機製與下調VEGF錶達有關.
목적 탐토이병분예선급약대지다당(LPS)유도대서신소구혈관내피세포통투성승고적영향.방법 분리、배양SD대서신소구혈관내피세포,이1×106/ml적밀도접충우24공배양판(200 μl/공)화transwell소실(100 μl/실),채용수궤수자표법,장기수궤분위6조(n=10),정상대조조(C조)불작임하처리;지방유대조조(I 조)가입10%지방유4 μg/ml;이병분조(P조)가입이병분4μg/ml;LPS조(L조):가입LPS 10μg/ml;LPS+지방유조(L+I조)가입10%지방유4 μg/ml급LPS 10μg/ml;LPS+이병분조(L+P조)가입이병분4μg/ml 급LPS 10 μg/ml.우가입LPS전30 min가입지방유혹이병분,약물적농도균위종농도.가입LPS후6 h,수집세포,채용역전록-취합매련반응측정혈관내피세포생장인자(VEGF)mRNA표체수평;수집상청액,채용매련면역흡부법측정VEGF농도;측정혈관내피세포통투성.결과 여C조비교,L조、L+I조화L+P조VEGF mRNA표체상조,상청액중VEGF농도화혈관내피세포통투성증가(P<0.05),이I조화P조상술지표차이무통계학의의(P>O.05).여L조비교,L+P조VEGF mRNA표체하조,상청액중VEGF농도화혈관내피세포통투성강저(P<0.05),L+I조상술지표차이무통계학의의(P>0.05).상청액중VEGF농도여혈관내피세포통투성정정상관(r=0.833,P<0.05).결론 이병분예선급약가억제LPS유도대서신소구혈관내피세포통투성승고,기궤제여하조VEGF표체유관.
Objective To investigate the influence of propofol pretreatment on the increased glomerular endothelial cell permeability induced by lipopolysaccharide (LPS) in rats.Methods Glomerular endothelial cells isolated from SD rats were cultured in 24-well plates(200 μl/well) and transwell filters (100 μl/filter) at 1×106/ml and assigned into 6 groups (n=10 each):control group (group C) , introlipid group (group I), propofol group (group P) , LPS group (group L), LPS+introlipid group (group L+I) and LPS+propofol group (group L +P). In group I, 10% introlipid 4 μg/ml was added. In group P, 4 μg/ml propofol was added. In group L, 10 μg/ml LPS was added. In group L+I, 10% introlipid 4 fig/ml combined with 10 μg/ml LPS was added. In group L+ P, 4 μg/ml propofol combined with LPS 10 μg/ml was added. Introlipid or propofol was added 30 min before the administration of LPS and the corresponding concentrations mentioned above were all final concentrations.After 6 h incubation with LPS, the cells were collected for measurement of vascular endothelial growth factor (VEGF) mRNA expression using RT-PCR. The supernatant was collected for determination of the VEGF concentration by ELJSA. The endothelial cell permeability was determined. Results Compared with group C, the expression of VEGF mRNA was up-regulated and the VEGF concentration and endothelial cell permeability were significantly increased in L, L+I and L + P groups (P<0.05 ) ,but no significant change was found in the parameters mentioned above in I and P groups (P>0.05). Compared with group L, the expression of VEGF mRNA was downregulated and the VEGF concentration and endothelial cell permeability were significantly decreased in L+P group (P<0.05), but no significant change was found in the parameters mentioned above in group L+I(P>0.05). A positive correlation existed between the concentration of VEGF and the permeability of endothelial cells(r= 0.833,P<0.05).Conclusion Propofol pretreatment can decrease the increased glomerular endothelial cell permeability induced by LPS probably through down-regulation of VEGF expression.