CAJ | 학술논문

目的评价吗啡对小鼠脑微血管内皮细胞P-糖蛋白(P-gp)表达的影响.方法小鼠脑微血管内皮细胞b.End3,培养于RPMI 1640无血清高糖培养基中,接种于10 cm培养皿中,分为3组,每组9皿,每皿2 ml,M组加入1 μg/ml吗啡,P+M组在加人吗啡前1 h加入5 μnol/L NF-κB抑制剂吡咯二硫氨基甲酸酯,药物浓度均为终浓度,C组不作药物处理.加入吗啡后24 h时收集细胞,测定P-gp和NF-κB p65-abcb1b DNA复合体的表达水平.结果与C组比较,M组P-gp和NF-κB p65-abcb1bDNA复合体的表达水平上调(P＜0.01);与M组比较,P+M组P-gp和NF-κB p65-abcb1b DNA复合体的表达水平下调(P＜0.05或0.01).结论吗啡可上调小鼠脑微血管内皮细胞P-gp表达,其机制与NF-κB介导的P-gp基因abcb1b激活有关.
목적 평개마배대소서뇌미혈관내피세포P-당단백(P-gp)표체적영향.방법 소서뇌미혈관내피세포b.End3,배양우RPMI 1640무혈청고당배양기중,접충우10 cm배양명중,분위3조,매조9명,매명2 ml,M조가입1 μg/ml마배,P+M조재가인마배전1 h가입5 μnol/L NF-κB억제제필각이류안기갑산지,약물농도균위종농도,C조불작약물처리.가입마배후24 h시수집세포,측정P-gp화NF-κB p65-abcb1b DNA복합체적표체수평.결과 여C조비교,M조P-gp화NF-κB p65-abcb1bDNA복합체적표체수평상조(P＜0.01);여M조비교,P+M조P-gp화NF-κB p65-abcb1b DNA복합체적표체수평하조(P＜0.05혹0.01).결론 마배가상조소서뇌미혈관내피세포P-gp표체,기궤제여NF-κB개도적P-gp기인abcb1b격활유관.
Objective To investigate the effect of morphine on P-glycoprotein (P-gp) expression in mouse brain microvascular endothelial cells. Methods The mouse brain microvascular endothelial cell line b. End3 was purchased from ATCC company (USA) and cultured at 37 ℃ in high glucose serum-free medium RPMI 1640 in 10 cm petri dishes and assigned to one of 3 groups(n = 9 each): Ⅰ control group (group C); Ⅱ morphine group (group M) and Ⅲ PDTC + morphine group (group P + M). In group M the cells were exposed to morphine 1 μg/ml while in group P + M the cells were pre-incubated with PDTC (NF-κB inhibitor) 5 μmol/L for 1 h before treatment with morphine. In group M and P+ M after being treated with morphine 1 μg/ml for 24 h, the cells were collected for determination of P-gp expression and NF-κB p65-abcb1b protein-DNA binding analysis. Results P-gP expression and NF-κB p65-abcb1b protein-DNA binding were up-regulated in group M as compared with group C. The up-regulation was negated by pre-incubation with PDTC in group P + M. Conclusion Morphine can induce up-regulation of the expression of endogenous P-gp in mouse brain microvascular endothelial cells by NF-κB mediated-abcb1 b gene activation.