CAJ | 학술논문

目的探讨七氟醚预处理对局灶性脑缺血再灌注损伤大鼠海马机械敏感性钾通道TREK-1表达的影响.方法健康雄性SD大鼠36只,体重240～280 g,随机分为3组(n=12):假手术组(S组)、局灶性脑缺血再灌注组(l/R组)和七氟醚预处理组(Sevo组).结扎右侧颈总动脉、颈外动脉,采用线栓法阻断颈内动脉2 h,再灌注24 h制备大鼠局灶性脑缺血再灌注损伤模型;Sevo组于缺血前1 h经半密闭的吸入箱持续吸入含2.5%七氟醚的02;S组仅分离并结扎右侧颈总动脉、颈外动脉,不置入线栓.各组于再灌注24 h时行神经功能缺陷评分后断头取脑,TIC染色后测定脑梗死体积,采用RT-PCR法测定海马TREK-1 mRNA的表达.结果与S组相比,I/R组和Sevo组神经功能缺陷评分和脑梗死体积比升高(P<0.01);与I/R组相比,Sevo组神经功能缺陷评分和脑梗死体积比降低,海马TREK-1 mRNA表达上调(P<0.05).结论七氟醚预处理可通过激活海马TREK-1减轻大鼠局灶性脑缺血再灌注损伤.
목적 탐토칠불미예처리대국조성뇌결혈재관주손상대서해마궤계민감성갑통도TREK-1표체적영향.방법 건강웅성SD대서36지,체중240～280 g,수궤분위3조(n=12):가수술조(S조)、국조성뇌결혈재관주조(l/R조)화칠불미예처리조(Sevo조).결찰우측경총동맥、경외동맥,채용선전법조단경내동맥2 h,재관주24 h제비대서국조성뇌결혈재관주손상모형;Sevo조우결혈전1 h경반밀폐적흡입상지속흡입함2.5%칠불미적02;S조부분리병결찰우측경총동맥、경외동맥,불치입선전.각조우재관주24 h시행신경공능결함평분후단두취뇌,TIC염색후측정뇌경사체적,채용RT-PCR법측정해마TREK-1 mRNA적표체.결과 여S조상비,I/R조화Sevo조신경공능결함평분화뇌경사체적비승고(P<0.01);여I/R조상비,Sevo조신경공능결함평분화뇌경사체적비강저,해마TREK-1 mRNA표체상조(P<0.05).결론 칠불미예처리가통과격활해마TREK-1감경대서국조성뇌결혈재관주손상.
Objective To investigate the effect of sevoflurane preconditioning on TREK-1 channel expression in hippocampus after focal cerebral ischemia-reperfusion (I/R)in rats and the mechanism.Methods Thirty-six male SD rats weighing 240-280 g were randomly divided into 3 groups(n=12 each):group Ⅰ sham operation (group S),group Ⅱ I/R and group Ⅲ sevoflurane preconditioning (group Sevo).Focal cerebral ischemia was produced by inserting a 4-0 nylon thread with rounded tip into right internal jugular vein.The nylon thread was the nylon thread Wag about 18-20 Innl.The right middle cerebral artery(MCA)Wag occluded for 2 h and then released for 24 h reperfusion.The Sevo group inhaled 2.4% sevoflurane for 30 min at l h before ischemia.Neurological deftcits were assessed and scored at the end of 24 h reperfusion (the higher was the score,the severer was the deficit).The cerebral infarct size was determined by TTC staining and the TREK-1 mRNA in hippocampus by RT-PCR.Results The cerebral infarct size was significantly smaller and the neurological deficit scores were significantly lower in Sevo group than in I/R group.The TREK-1 mBNA expression was significantly up-regulated in Sevo group as compared with I/R group.Conclusion Sevoflurane preconditioning Can protect the brain against I/R injury by activating TREK-1 in hippocampus.