中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
6期
463-465
,共3页
张烨琼%卢建溪%孙海霞%舒欣%曹红%潘兴飞%徐启桓%李刚
張燁瓊%盧建溪%孫海霞%舒訢%曹紅%潘興飛%徐啟桓%李剛
장엽경%로건계%손해하%서흔%조홍%반흥비%서계환%리강
癌,肝细胞%肝炎病毒,乙型%成纤维细胞生长因子
癌,肝細胞%肝炎病毒,乙型%成纖維細胞生長因子
암,간세포%간염병독,을형%성섬유세포생장인자
Carcinoma,hepatocellular%Hepatitis B virus%Fibroblast growth factors%Hepatocytes
目的 研究成纤维细胞激活蛋白(FAP)在乙肝相关性肝癌中的表达,探讨其临床意义.方法 采用反转录聚合酶链反应(RT-PCR)检测33例乙肝相关性肝癌患者手术切除肝癌组织、癌旁组织及远隔相对正常肝组织及13例正常肝组织FAP表达情况,同时采用荧光定量PCR方法检测其mRNA表达差异.结果 FAP mRNA在肝癌组织、癌旁组织以及远隔相对正常肝组织中均有表达,相对于对照组正常肝组织的差异倍数值分别为5.14 ±6.69、1.58±0.96、1.63 ±0.94,三者比较差异有统计学意义(F=4.401,P＜0.05)；FAP mRNA的差异倍数值在Ⅰ期、Ⅱ期、Ⅲ期肝癌中分别为2.89±3.35、4.15±4.69、10.09 ±9.51,在高、中、低分化肝癌中分别为1.62±1.74、3.84±3.79、11.26±13.34,差异均有统计学意义(P＜0.05).结论 FAP可能在乙肝相关性肝癌的发生、发展中发挥重要作用,可作为乙肝相关性肝癌分化程度及预后判断的指标.
目的 研究成纖維細胞激活蛋白(FAP)在乙肝相關性肝癌中的錶達,探討其臨床意義.方法 採用反轉錄聚閤酶鏈反應(RT-PCR)檢測33例乙肝相關性肝癌患者手術切除肝癌組織、癌徬組織及遠隔相對正常肝組織及13例正常肝組織FAP錶達情況,同時採用熒光定量PCR方法檢測其mRNA錶達差異.結果 FAP mRNA在肝癌組織、癌徬組織以及遠隔相對正常肝組織中均有錶達,相對于對照組正常肝組織的差異倍數值分彆為5.14 ±6.69、1.58±0.96、1.63 ±0.94,三者比較差異有統計學意義(F=4.401,P＜0.05)；FAP mRNA的差異倍數值在Ⅰ期、Ⅱ期、Ⅲ期肝癌中分彆為2.89±3.35、4.15±4.69、10.09 ±9.51,在高、中、低分化肝癌中分彆為1.62±1.74、3.84±3.79、11.26±13.34,差異均有統計學意義(P＜0.05).結論 FAP可能在乙肝相關性肝癌的髮生、髮展中髮揮重要作用,可作為乙肝相關性肝癌分化程度及預後判斷的指標.
목적 연구성섬유세포격활단백(FAP)재을간상관성간암중적표체,탐토기림상의의.방법 채용반전록취합매련반응(RT-PCR)검측33례을간상관성간암환자수술절제간암조직、암방조직급원격상대정상간조직급13례정상간조직FAP표체정황,동시채용형광정량PCR방법검측기mRNA표체차이.결과 FAP mRNA재간암조직、암방조직이급원격상대정상간조직중균유표체,상대우대조조정상간조직적차이배수치분별위5.14 ±6.69、1.58±0.96、1.63 ±0.94,삼자비교차이유통계학의의(F=4.401,P＜0.05)；FAP mRNA적차이배수치재Ⅰ기、Ⅱ기、Ⅲ기간암중분별위2.89±3.35、4.15±4.69、10.09 ±9.51,재고、중、저분화간암중분별위1.62±1.74、3.84±3.79、11.26±13.34,차이균유통계학의의(P＜0.05).결론 FAP가능재을간상관성간암적발생、발전중발휘중요작용,가작위을간상관성간암분화정도급예후판단적지표.
Objective To analyze the gene expression level of fibroblast activation protein in HBV related hepatocellular carcinoma patients and discuss its clinical significance.Methods FAP gene expression in 33 hepatocellular carcinoma patients cancer tissues,peficancerous tissues,distant relative normal liver tissues and 13 normal liver tissues were examined by reverse transcription PCR;and real-time fluorescent quantitative PCR (qRT-PCR) was used to quantify their expression.Results FAP were expressed in all the tissues,the relative expression values in cancer tissues,peficancerous tissues and distant relative normal liver tissues were 5.14 ± 6.69,1.58 ± 0.96,1.63 ± 0.94,respectively,the differences were statistically significant ( F =4.401,P ＜ 0.05 ) ; and in TN M stage Ⅰ,Ⅱ,Ⅲ,they were 2.89 ± 3.35,4.15 ±4.69,10.09 ± 9.51 respectively; in well - differentiated,differentiated and poorly differentiated hepatocellular carcinoma were 1.62 ± 1.74,3.84 ±3.79,11.26 ± 13.34respectively.The differences were all statistically significant( P ＜ 0.05 ).Conclusion FAP may play an important role in the occurrence and development of HBV related hepatocellular carcinoma.
