CAJ | 학술논문

目的明确我国汉族人群中子宫内膜异位症(EMS)患者与肿瘤坏死因子(TNF)的基因多态性之间的关系,探讨子宫内膜异位症的发病机制.方法采用聚合酶链式反应-限制性片段长度多态性技术对87例正常人群(对照组)与76例EMS患者的TNFα和TNFβ基因的单核苷酸多态性进行了分析.结果 EMS组和对照组中,TNFα基因的野生型等位基因频率分别为0.9474和0.9253,突变型等位基因的频率(%)分别为0.0526和0.0747;TNFβ基因的野生型等位基因频率(%)分别为0.4605和0.5115,突变型等位基因的频率(%)分别为0.5395和0.4885.对各等位基因频率分别进行卡方检验,均P＞0.05.结论 TNF的基因多态性可能不是中国妇女内异症发病的危险因素.TNF的等位基因频率可能存在人种差异.
목적 명학아국한족인군중자궁내막이위증(EMS)환자여종류배사인자(TNF)적기인다태성지간적관계,탐토자궁내막이위증적발병궤제.방법 채용취합매련식반응-한제성편단장도다태성기술대87례정상인군(대조조)여76례EMS환자적TNFα화TNFβ기인적단핵감산다태성진행료분석.결과 EMS조화대조조중,TNFα기인적야생형등위기인빈솔분별위0.9474화0.9253,돌변형등위기인적빈솔(%)분별위0.0526화0.0747;TNFβ기인적야생형등위기인빈솔(%)분별위0.4605화0.5115,돌변형등위기인적빈솔(%)분별위0.5395화0.4885.대각등위기인빈솔분별진행잡방검험,균P＞0.05.결론 TNF적기인다태성가능불시중국부녀내이증발병적위험인소.TNF적등위기인빈솔가능존재인충차이.
Objective To evaluate the association between the susceptibility of endometriosis(EMS)and the gene polymorphism of tumor necrosis factor(TNF)in Chinese Han population.Methods Peripheral blood samples were collected from 76 EMS patients and 87 normal controls.PCR-RFLP was used to detect the single base change polymorphism in both TNFα gene and TNFβ gene.Results The allele frequencies of the TNFα pdymorphism were 0.9474 and 0.9253 in the EMS patients and the control group respectively,and the TNFβ polymorphism were 0.0526 and 0.0747 respectively.The allele frequencies of the TNFβ1polymorphism were 0.4605 and 0.5115 in the EMS patients and the control group,and the TNFβ2polymorphism were 0.5395 and 0.4885.No difference in the gene and genotype frequencies of TNFα and TNFβ was noted in the EMS patients and normal controls(all P＞0.05).Conclusion TNF gene polymorphism has no effect on EMS in Chinese Han population.Ethnic difference may exist in TNF allele frequency.