CAJ | 학술논문

目的利用突变敏感性分子开关技术检测乳腺癌磷脂酰肌醇-3-激酶催化亚单位α(PIK3CA)基因A3140G突变频率,以便建立快速检测基因突变的技术方法.方法用高保真DNA聚合酶介导的突变敏感性分子开关技术对62例乳腺癌组织和10例乳腺纤维腺瘤组织DNA进行检测,并利用凝胶成像系统对结果进行分析.结果在62例乳腺癌患者中检测出10例PIK3CA基因A3140G突变(突变率为16.1％),10例乳腺纤维腺瘤中未发现该突变.PIK3CA基因A3140G突变与年龄、肿瘤大小、淋巴结状态、雌激素受体(ER)/孕激素受体(PR)状态、表皮生长因子受体-2(cerbB-2)和表皮生长因子受体(EGFR)表达等无相关.结论突变敏感性分子开关技术可快速准确地检测乳腺癌PIK3CA基因A3140G突变.
목적 이용돌변민감성분자개관기술검측유선암린지선기순-3-격매최화아단위α(PIK3CA)기인A3140G돌변빈솔,이편건립쾌속검측기인돌변적기술방법.방법 용고보진DNA취합매개도적돌변민감성분자개관기술대62례유선암조직화10례유선섬유선류조직DNA진행검측,병이용응효성상계통대결과진행분석.결과 재62례유선암환자중검측출10례PIK3CA기인A3140G돌변(돌변솔위16.1％),10례유선섬유선류중미발현해돌변.PIK3CA기인A3140G돌변여년령、종류대소、림파결상태、자격소수체(ER)/잉격소수체(PR)상태、표피생장인자수체-2(cerbB-2)화표피생장인자수체(EGFR)표체등무상관.결론 돌변민감성분자개관기술가쾌속준학지검측유선암PIK3CA기인A3140G돌변.
Objective To establish a technology platform which can screen the breast cancer phosphatidylinositol 3-kinase catalytic alpha (PIK3CA) gene A3140G mutation rapidly by using the mutation-sensitive molecular switch.Methods The A3140G mutation of PIK3CA gene was detected by the fidelity DNA polymerase-mediated mutation-sensitive molecular switch in 62 samples of breast cancer and 10 samples of breast fibroadenoma.Results Ten ( 16.1％ ) A314OG mutations were found in these 62 cases of breast cancer and no mutation was found in 10 cases of breast fibroadenoma.The A3140G mutation of PIK3CA gene had no significant correlation with the age,tumor size,lymph node status,estrogen receptor ( ER)/progesterone receptor (PR) status,epidermal growth factor receptor 2 ( cerbB-2 ) and epidermal growth factor receptor ( EGFR ) expression.Conclusion The mutation-sensitive molecular switch can screen the breast cancer PIK3CA gene A3140G mutation rapidly and accurately.The technology of molecular switch has great clinically applied value in screening breast cancer gene mutation.