中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
2期
86-90
,共5页
杜建青%彭然%许丹焰%赵婷婷%沈莉%赵水平
杜建青%彭然%許丹燄%趙婷婷%瀋莉%趙水平
두건청%팽연%허단염%조정정%침리%조수평
冠状动脉疾病%环氧化合物%脂肪酸合成酶%炎症
冠狀動脈疾病%環氧化閤物%脂肪痠閤成酶%炎癥
관상동맥질병%배양화합물%지방산합성매%염증
Coronary syndrome%Epoxiy compounds%Fatty acid synthase%Inflamma somes
目的 探讨可溶性环氧化物水解酶抑制剂(sEHi)对急性冠脉综合征(ACS)患者外周血单个核细胞脂肪酸合酶(FASN)表达的影响.方法 入选ACS患者35例为ACS组,体检健康者30名为对照组.于入院后24h内分别测定血脂谱、空腹血糖、心肌酶谱和超敏C反应蛋白(hs-CRP).同时分离培养外周血单个核细胞,以不同浓度可溶性环氧化物水解酶抑制剂(t-AUCB)干预,用实时荧光定量PCR、蛋白印迹法分别测定FASN及IL-6的mRNA及蛋白表达水平.结果 (1)ACS组血清hs-CRP浓度为(5.6±4.1)mg/L,与对照组(1.3±0.9 )mg/L比较显著升高,差异有统计学意义(P＜0.05)；与对照组比较,ACS组单个核细胞内FASN、IL-6 mRNA及蛋白的表达水平均显著升高(相对表达量:FASN mRNA:1比1.709±0.272,FASN蛋白:0.407±0.065比1.298±0.087;IL-6mRNA:1比2.302±0.200,IL-6蛋白:0.715±0.058比1.146±0.083,P＜0.05),且FASN、IL-6 mRNA表达量分别与血清hs-CRP浓度呈正相关(r=0.714,P＜0.01；r=0.685,P＜0.01).(2) t-AUCB可呈剂量依赖性抑制ACS组单个核细胞的FASN和IL-6 mRNA与蛋白表达,t-AUCB干预组与空白对照组(t-AUCB)比较,差异均有统计学意义(均P＜0.05)(其中t-AUCB 0、10、50、100 μmol/L组中,FASNmRNA相对表达量分别为:1,0.813±0.038,0.564±0.100,0.293±0.043;FASN分别为:0.957±0.280,0.935±0.275,0.855±0.253,0.685±0.206.结论 ACS患者体内有较高的炎症水平,其外周血单个核细胞中FASN的表达水平与体内炎症反应密切相关,t-AUCB抑制单个核细胞内的FASN表达及炎症反应,可能起到稳定斑块的作用.
目的 探討可溶性環氧化物水解酶抑製劑(sEHi)對急性冠脈綜閤徵(ACS)患者外週血單箇覈細胞脂肪痠閤酶(FASN)錶達的影響.方法 入選ACS患者35例為ACS組,體檢健康者30名為對照組.于入院後24h內分彆測定血脂譜、空腹血糖、心肌酶譜和超敏C反應蛋白(hs-CRP).同時分離培養外週血單箇覈細胞,以不同濃度可溶性環氧化物水解酶抑製劑(t-AUCB)榦預,用實時熒光定量PCR、蛋白印跡法分彆測定FASN及IL-6的mRNA及蛋白錶達水平.結果 (1)ACS組血清hs-CRP濃度為(5.6±4.1)mg/L,與對照組(1.3±0.9 )mg/L比較顯著升高,差異有統計學意義(P＜0.05)；與對照組比較,ACS組單箇覈細胞內FASN、IL-6 mRNA及蛋白的錶達水平均顯著升高(相對錶達量:FASN mRNA:1比1.709±0.272,FASN蛋白:0.407±0.065比1.298±0.087;IL-6mRNA:1比2.302±0.200,IL-6蛋白:0.715±0.058比1.146±0.083,P＜0.05),且FASN、IL-6 mRNA錶達量分彆與血清hs-CRP濃度呈正相關(r=0.714,P＜0.01；r=0.685,P＜0.01).(2) t-AUCB可呈劑量依賴性抑製ACS組單箇覈細胞的FASN和IL-6 mRNA與蛋白錶達,t-AUCB榦預組與空白對照組(t-AUCB)比較,差異均有統計學意義(均P＜0.05)(其中t-AUCB 0、10、50、100 μmol/L組中,FASNmRNA相對錶達量分彆為:1,0.813±0.038,0.564±0.100,0.293±0.043;FASN分彆為:0.957±0.280,0.935±0.275,0.855±0.253,0.685±0.206.結論 ACS患者體內有較高的炎癥水平,其外週血單箇覈細胞中FASN的錶達水平與體內炎癥反應密切相關,t-AUCB抑製單箇覈細胞內的FASN錶達及炎癥反應,可能起到穩定斑塊的作用.
목적 탐토가용성배양화물수해매억제제(sEHi)대급성관맥종합정(ACS)환자외주혈단개핵세포지방산합매(FASN)표체적영향.방법 입선ACS환자35례위ACS조,체검건강자30명위대조조.우입원후24h내분별측정혈지보、공복혈당、심기매보화초민C반응단백(hs-CRP).동시분리배양외주혈단개핵세포,이불동농도가용성배양화물수해매억제제(t-AUCB)간예,용실시형광정량PCR、단백인적법분별측정FASN급IL-6적mRNA급단백표체수평.결과 (1)ACS조혈청hs-CRP농도위(5.6±4.1)mg/L,여대조조(1.3±0.9 )mg/L비교현저승고,차이유통계학의의(P＜0.05)；여대조조비교,ACS조단개핵세포내FASN、IL-6 mRNA급단백적표체수평균현저승고(상대표체량:FASN mRNA:1비1.709±0.272,FASN단백:0.407±0.065비1.298±0.087;IL-6mRNA:1비2.302±0.200,IL-6단백:0.715±0.058비1.146±0.083,P＜0.05),차FASN、IL-6 mRNA표체량분별여혈청hs-CRP농도정정상관(r=0.714,P＜0.01；r=0.685,P＜0.01).(2) t-AUCB가정제량의뢰성억제ACS조단개핵세포적FASN화IL-6 mRNA여단백표체,t-AUCB간예조여공백대조조(t-AUCB)비교,차이균유통계학의의(균P＜0.05)(기중t-AUCB 0、10、50、100 μmol/L조중,FASNmRNA상대표체량분별위:1,0.813±0.038,0.564±0.100,0.293±0.043;FASN분별위:0.957±0.280,0.935±0.275,0.855±0.253,0.685±0.206.결론 ACS환자체내유교고적염증수평,기외주혈단개핵세포중FASN적표체수평여체내염증반응밀절상관,t-AUCB억제단개핵세포내적FASN표체급염증반응,가능기도은정반괴적작용.
Objective To explore the effects of soluble epoxide hydrolase inhibitor (sEHi) on the expressions of fatty acid synthase (FASN) mRNA and protein in peripheral blood mononuclear cell (PBMCs) of patients with acute coronary syndrome (ACS) and to discuss the influences of sEHi in the regulated expression of FASN and inflammatory reaction.Methods The hospitalized ACS patients were selected as the ACS group (n =35 ) while the healthy normal subjects as the control group (n =30).The levels of lipoproteins,fasting blood glucose,myocardial enzyme and hs-CRP (high-sensitive C-reactive protein) were measured within 24 hours after admission.Meanwhile the PBMCs were separated and cultured and then t-AUCB was added in various concentrations (0,1,10,50,100 μmol/L). The cellular expressions of FASN,IL-6 mRNA and protein were detected by real-time PCR (polymerase chain reaction)and Western blot respectively.Results ( 1 )The serum concentration of hs-CRP reached (5.6 ± 4.1 )mg/L in the ACS group.And it was obviously higher than ( 1.3 ± 0.9 ) mg/L in the control group ( P ＜ 0.05 ).Compared with the control group,the expression levels of FASN,IL-6 mRNA and protein significantly increased in the ACS group ( the relative expression amount of FASN mRNA: 1 vs 1.709 ± 0.272,FASN protein: 0.407 ± 0.065 vs 1.298 ± 0.087 ; relative expression amount of IL-6 mRNA: 1 vs 2.302 ± 0.200,IL-6 protein: 0.715 ± 0.058 vs 1.146 ± 0.083,P ＜ 0.05 ).Moreover,the levels of FASN and IL-6 mRNA had positive correlations with the serum concentration of hs-CRP ( r =0.714,P ＜ 0.01 ; r =0.685,P ＜0.01).(2) Compared with the control group (t-AUCB 0 μmol/L),10,50,100 μmol/L t-AUCB had inhibited the expressions quantity of FASN,IL,-6 mRNA and protein in PBMCs from the ACS group (P ＜ 0.05 ).The relative expressions of FASN mRNA in t-AUCB 0,10,50,100 μmol/L group were 1,0.813 ± 0.038,0.564 ±0.100,0.293 ±0.043 respectively.The relative expressions of FASN protein in t-AUCB 0,10,50 and 100 μmol/L group were 0.957 ± 0.280,0.935 ± 0.275,0.855 ± 0.253,0.685 ± 0.206respectively.Conclusion The inflammatory level increases obviously in the ACS group.And the expression level of FASN in PBMCs is closely correlated with the inflammatory level in the ACS patients; t-AUCB may prevent the ruptures of atherosclerotic lesions by regulating FASN and inhibiting inflammatory reactions in ACS patients.
