CAJ | 학술논문

动力蛋白(dynamin)是一类具有可被微管激活的GTP酶活性的新型马达蛋白,被证明在动物细胞受体介导的内吞小泡的形成、突触小泡再循环及高尔基体的囊泡运输中起关键作用.近几年,一些植物细胞也被发现有动力蛋白类似物.本研究通过分子量鉴定和免疫印迹法证明萱草(Hemerocallis fulva L.)花粉中存在动力蛋白,其分子量为100 kD;经过高度纯化的花粉动力蛋白仍具有GTPase活性,且可被牛脑微管激活1.64倍;电子显微镜观察结果表明,花粉动力蛋白可自我组装成环状结构.
동력단백(dynamin)시일류구유가피미관격활적GTP매활성적신형마체단백,피증명재동물세포수체개도적내탄소포적형성、돌촉소포재순배급고이기체적낭포운수중기관건작용.근궤년,일사식물세포야피발현유동력단백유사물.본연구통과분자량감정화면역인적법증명훤초(Hemerocallis fulva L.)화분중존재동력단백,기분자량위100 kD;경과고도순화적화분동력단백잉구유GTPase활성,차가피우뇌미관격활1.64배;전자현미경관찰결과표명,화분동력단백가자아조장성배상결구.
Dynamin,a 100-kD GTPase first found in animal cells,is essential for vesicle formation in receptor-mediated endocytosis,synaptic vesicle recycling,and possibly vesicle trafficking in and out of the Golgi apparatus.Recently,dynamin-like proteins were also found in some plant cells.We demonstrate here the presence of dynamin with molecular weight of 100 kD in day-lily ( Hemerocallis fulva ) pollen based on molecular estimation and Western blotting.The highly purified pollen Dynamin had GTPase activity,which could be stimulated 1.64 fold by calf brain microtubules in vitro.The results from electron microscopic examination showed that the pollen dynamin readily self-assembled into ring-like structures.