CAJ | 학술논문

植物激素ABA参与不同的生理过程,尤其是在种子发育和非生物逆境的适应都需要ABA的调控.以水稻珍汕97和旱稻IRAT109为亲本的重组自交系群体为材料,分别调查种子发芽和苗期对ABA的敏感程度.种子发芽阶段以ABA处理下的相对发芽势(Relative germination vigor,RGV)和相对发芽率(Relative germination rate,RGR)为指标,苗期以ABA喷施处理下的卷叶程度(Leaf rolling scores by ABA spaying,LRS)和含ABA水培条件下的卷叶程度(Leaf rolling scores by ABA culturing,LRC)为指标.性状相关分析表明发芽阶段的相对发芽势与苗期卷叶程度呈显著正相关.用复合区间作图法和混合线性模型对ABA敏感性QTL定位和上位性效应分析.两种软件检测到的主效QTL位点大致相同.共检测到5个单位点QTL和6对上位性QTL与发芽阶段的ABA敏感性有关;8个单位点QTL和5对上位性QTL与水稻苗期对ABA的敏感性有关;在苗期,两种ABA处理条件下共检测到两个共同的QTL;仅一个共同的QTL同时控制发芽阶段和苗期对ABA的敏感性.这些研究结果说明,水稻对ABA的敏感性同时受单位点的多基因和上位性基因控制;而且控制种子萌发阶段发芽势和苗期对ABA敏感性的遗传基础有很大的不同.
식물격소ABA삼여불동적생리과정,우기시재충자발육화비생물역경적괄응도수요ABA적조공.이수도진산97화한도IRAT109위친본적중조자교계군체위재료,분별조사충자발아화묘기대ABA적민감정도.충자발아계단이ABA처리하적상대발아세(Relative germination vigor,RGV)화상대발아솔(Relative germination rate,RGR)위지표,묘기이ABA분시처리하적권협정도(Leaf rolling scores by ABA spaying,LRS)화함ABA수배조건하적권협정도(Leaf rolling scores by ABA culturing,LRC)위지표.성상상관분석표명발아계단적상대발아세여묘기권협정도정현저정상관.용복합구간작도법화혼합선성모형대ABA민감성QTL정위화상위성효응분석.량충연건검측도적주효QTL위점대치상동.공검측도5개단위점QTL화6대상위성QTL여발아계단적ABA민감성유관;8개단위점QTL화5대상위성QTL여수도묘기대ABA적민감성유관;재묘기,량충ABA처리조건하공검측도량개공동적QTL;부일개공동적QTL동시공제발아계단화묘기대ABA적민감성.저사연구결과설명,수도대ABA적민감성동시수단위점적다기인화상위성기인공제;이차공제충자맹발계단발아세화묘기대ABA민감성적유전기출유흔대적불동.
Abscisic acid (ABA) is one of the important plant hormones, which plays a critical role in seed development and adaptation to abiotic stresses. The sensitivity of rice (Oryza sativa L.) to exogenous ABA at seed germination and seedling stages was investigated in the recombinant inbred line (RIL) population derived from a cross between irrigated rice Zhenshan 97 and upland rice IRAT109, using relative germination vigor (RGV), relative germination rate (RGR) and leaf rolling scores of spraying (LRS) or culturing (LRC) with ABA as sensitivity indexes. The phenotypic correlation analysis revealed that only RGV at germination stage was positively correlated to ABA sensitivity at seedling stage. QTL detection using composite interval mapping (CIM) and mixed linear model was conducted to dissect the genetic basis of ABA sensitivity, and the single-locus QTLs detected by both methods are in good agreement with each other. Five single QTLs and six pairs of epistatic QTLs were detected for ABA sensitivity at germination stage. Eight single QTLs and five pairs of epistatic QTLs were detected for ABA sensitivity at seedling stage. Two QTLs were common between LRS and LRC; and one common QTL was detected for RGV, LRS and LRC simultaneously. These results indicated that both single and epistatic loci were involved in the ABA sensitivity in rice, and the genetic basis of ABA sensitivity at seed germination and seedling stage was largely different.