背景:作者曾提出:"运动中有氧产能过程与当时耗能过程不匹配是代谢发生转变的原因;而丙酮酸转化成乳酸的直接作用是防止丙酮酸在胞浆内堆积,防止其堆积对糖酵解产能过程的抑制,以保证酵解过程的快速供能;这一步生化反应的机制是对畅通糖代谢酵解途径供能速率的调节"的假说.目的:观察补充吸氧对代谢转变有无影响,分别从人体和动物水平上探讨乳酸阈强度(代谢转变时)下代谢转变的机制,验证人体和动物结果的一致性.设计:随机对照观察.单位:河北师范大学体育学院,廊坊师范学院体育系.对象:受试者为24名体育专业本科男生,体质量为(58±4)kg,身高为(175±6)Gm,年龄(21±2)岁,二级运动员12人,无等级学生12人;雄性SD大鼠30只.方法:整体实验于2006-04/06河北师范大学体育学院运动生理机能实验室完成.24名学生分为二级运动员组和无等级训练组,各12名,进行递增负荷功率自行车运动;选取30只SD大鼠随机分为负重游泳训练组15只,无负重游泳适应组15只,负重游泳训练组进行递增负荷游泳运动.首先确定人体组与大鼠组各自的代谢转变强度,后在正常吸空气与补充吸氧条件下重复其前一阶段运动.分别在重复运动前及递增负荷运动到乳酸阈强度下,测定人体及大鼠的静脉血氧分压、丙酮酸、乳酸含量.人体组每2 min递增负荷50 W,大鼠组每2 min递增负荷是体质量的1%,直至不能坚持为止.选择递增负荷运动方法,让体内代谢逐步由有氧向无氧代谢过度,确定过度点即乳酸阈强度.通过静脉血氧分压、丙酮酸、乳酸含量的前后对比和补充与否对各指标有无影响,以及人体和动物的结果是否一致,以证明假说的信度和效度.主要观察指标:人体组和大鼠组乳酸阈强度下及补充吸氧前后的静脉血氧分压、丙酮酸、乳酸含量.结果:受试者24名和30只大鼠全部进入结果分析.①人体受试者(两组)和30只大鼠(两组)在递增负荷运动中每级负荷2 min末取血所得到的乳酸曲线,明显反映出了血乳酸拐点所对应的代谢转变强度及训练水平的差异,有训练者的血乳酸拐点明显置后.②在乳酸阈强度下,不论是否吸氧,人体组和大鼠组的血乳酸含量与氧分压之间均不相关[(3.61±0.56),(5.43±0.55)mmol/L;(4.46±0.86),(7.80±0.27)kPa,r=0.31,0.31,P＞0.05],整个测试过程人体组血氧饱和度均不低于98%;而两组受试血乳酸与血丙酮酸含量之间差异均有非常显著性意义[丙酮酸:(1.04±0.16),(0.91±0.37)mmol/L,P＜0.001].③人体组和大鼠组在重复运动前及乳酸阈强度下,丙酮酸平均值分别是(0.97±0.17),(1.04±0.16)mmol/L;(0.93±0.25),(0.91±0.37)mmol/L.两组受试重复运动前与乳酸阈强度时的血丙酮酸含量差异均无显著性意义(P＞0 05).结论:运动中由有氧向无氧代谢转变时体内不缺氧,补充吸氧对代谢的转变没有影响;丙酮酸不易通过肌细胞膜而乳酸可以通过.实验结果支持丙酮酸转化成乳酸的直接作用是防止丙酮酸在胞浆内堆积的观点.
揹景:作者曾提齣:"運動中有氧產能過程與噹時耗能過程不匹配是代謝髮生轉變的原因;而丙酮痠轉化成乳痠的直接作用是防止丙酮痠在胞漿內堆積,防止其堆積對糖酵解產能過程的抑製,以保證酵解過程的快速供能;這一步生化反應的機製是對暢通糖代謝酵解途徑供能速率的調節"的假說.目的:觀察補充吸氧對代謝轉變有無影響,分彆從人體和動物水平上探討乳痠閾彊度(代謝轉變時)下代謝轉變的機製,驗證人體和動物結果的一緻性.設計:隨機對照觀察.單位:河北師範大學體育學院,廊坊師範學院體育繫.對象:受試者為24名體育專業本科男生,體質量為(58±4)kg,身高為(175±6)Gm,年齡(21±2)歲,二級運動員12人,無等級學生12人;雄性SD大鼠30隻.方法:整體實驗于2006-04/06河北師範大學體育學院運動生理機能實驗室完成.24名學生分為二級運動員組和無等級訓練組,各12名,進行遞增負荷功率自行車運動;選取30隻SD大鼠隨機分為負重遊泳訓練組15隻,無負重遊泳適應組15隻,負重遊泳訓練組進行遞增負荷遊泳運動.首先確定人體組與大鼠組各自的代謝轉變彊度,後在正常吸空氣與補充吸氧條件下重複其前一階段運動.分彆在重複運動前及遞增負荷運動到乳痠閾彊度下,測定人體及大鼠的靜脈血氧分壓、丙酮痠、乳痠含量.人體組每2 min遞增負荷50 W,大鼠組每2 min遞增負荷是體質量的1%,直至不能堅持為止.選擇遞增負荷運動方法,讓體內代謝逐步由有氧嚮無氧代謝過度,確定過度點即乳痠閾彊度.通過靜脈血氧分壓、丙酮痠、乳痠含量的前後對比和補充與否對各指標有無影響,以及人體和動物的結果是否一緻,以證明假說的信度和效度.主要觀察指標:人體組和大鼠組乳痠閾彊度下及補充吸氧前後的靜脈血氧分壓、丙酮痠、乳痠含量.結果:受試者24名和30隻大鼠全部進入結果分析.①人體受試者(兩組)和30隻大鼠(兩組)在遞增負荷運動中每級負荷2 min末取血所得到的乳痠麯線,明顯反映齣瞭血乳痠枴點所對應的代謝轉變彊度及訓練水平的差異,有訓練者的血乳痠枴點明顯置後.②在乳痠閾彊度下,不論是否吸氧,人體組和大鼠組的血乳痠含量與氧分壓之間均不相關[(3.61±0.56),(5.43±0.55)mmol/L;(4.46±0.86),(7.80±0.27)kPa,r=0.31,0.31,P＞0.05],整箇測試過程人體組血氧飽和度均不低于98%;而兩組受試血乳痠與血丙酮痠含量之間差異均有非常顯著性意義[丙酮痠:(1.04±0.16),(0.91±0.37)mmol/L,P＜0.001].③人體組和大鼠組在重複運動前及乳痠閾彊度下,丙酮痠平均值分彆是(0.97±0.17),(1.04±0.16)mmol/L;(0.93±0.25),(0.91±0.37)mmol/L.兩組受試重複運動前與乳痠閾彊度時的血丙酮痠含量差異均無顯著性意義(P＞0 05).結論:運動中由有氧嚮無氧代謝轉變時體內不缺氧,補充吸氧對代謝的轉變沒有影響;丙酮痠不易通過肌細胞膜而乳痠可以通過.實驗結果支持丙酮痠轉化成乳痠的直接作用是防止丙酮痠在胞漿內堆積的觀點.
배경:작자증제출:"운동중유양산능과정여당시모능과정불필배시대사발생전변적원인;이병동산전화성유산적직접작용시방지병동산재포장내퇴적,방지기퇴적대당효해산능과정적억제,이보증효해과정적쾌속공능;저일보생화반응적궤제시대창통당대사효해도경공능속솔적조절"적가설.목적:관찰보충흡양대대사전변유무영향,분별종인체화동물수평상탐토유산역강도(대사전변시)하대사전변적궤제,험증인체화동물결과적일치성.설계:수궤대조관찰.단위:하북사범대학체육학원,랑방사범학원체육계.대상:수시자위24명체육전업본과남생,체질량위(58±4)kg,신고위(175±6)Gm,년령(21±2)세,이급운동원12인,무등급학생12인;웅성SD대서30지.방법:정체실험우2006-04/06하북사범대학체육학원운동생리궤능실험실완성.24명학생분위이급운동원조화무등급훈련조,각12명,진행체증부하공솔자행차운동;선취30지SD대서수궤분위부중유영훈련조15지,무부중유영괄응조15지,부중유영훈련조진행체증부하유영운동.수선학정인체조여대서조각자적대사전변강도,후재정상흡공기여보충흡양조건하중복기전일계단운동.분별재중복운동전급체증부하운동도유산역강도하,측정인체급대서적정맥혈양분압、병동산、유산함량.인체조매2 min체증부하50 W,대서조매2 min체증부하시체질량적1%,직지불능견지위지.선택체증부하운동방법,양체내대사축보유유양향무양대사과도,학정과도점즉유산역강도.통과정맥혈양분압、병동산、유산함량적전후대비화보충여부대각지표유무영향,이급인체화동물적결과시부일치,이증명가설적신도화효도.주요관찰지표:인체조화대서조유산역강도하급보충흡양전후적정맥혈양분압、병동산、유산함량.결과:수시자24명화30지대서전부진입결과분석.①인체수시자(량조)화30지대서(량조)재체증부하운동중매급부하2 min말취혈소득도적유산곡선,명현반영출료혈유산괴점소대응적대사전변강도급훈련수평적차이,유훈련자적혈유산괴점명현치후.②재유산역강도하,불론시부흡양,인체조화대서조적혈유산함량여양분압지간균불상관[(3.61±0.56),(5.43±0.55)mmol/L;(4.46±0.86),(7.80±0.27)kPa,r=0.31,0.31,P＞0.05],정개측시과정인체조혈양포화도균불저우98%;이량조수시혈유산여혈병동산함량지간차이균유비상현저성의의[병동산:(1.04±0.16),(0.91±0.37)mmol/L,P＜0.001].③인체조화대서조재중복운동전급유산역강도하,병동산평균치분별시(0.97±0.17),(1.04±0.16)mmol/L;(0.93±0.25),(0.91±0.37)mmol/L.량조수시중복운동전여유산역강도시적혈병동산함량차이균무현저성의의(P＞0 05).결론:운동중유유양향무양대사전변시체내불결양,보충흡양대대사적전변몰유영향;병동산불역통과기세포막이유산가이통과.실험결과지지병동산전화성유산적직접작용시방지병동산재포장내퇴적적관점.
BACKGROUND; Authors have proposed the hypothesis that, the mechanism change may result in the mismatch between the energy production and energy consumption during the aerobic exercise, and pyruvate can be transformed into lactic acid, which may prevent the accumulation of pyruvate in cytoplasm and in the energy production of glycolysis so as to ensure the fast energy supply in zymolysis; the mechanism of this biochemical event may be the adjustment of energizing velocity via glycomechanism zymolysis.OBJECTIVE: To observe the effect of oxygen inhalation on metabolic transition, study the mechanism of metabolic transition under the lactate threshold intensity in human body and animal, and verify the result consistency between the two.DESIGN: Randomized control observation.SETTING: Department of Physical Education, Hebei Normal University; Department of Physical Education, Langfang Teachers College.PARTICIPANTS: A total of 24 male university students majoring physical education were adopted, weight (58±4) kg,height (175±6) cm, age (21 ±2) years. They were consisted of 12 Level B national athletes and12 common students.Additionally 30 SD male rats were used.METHODS: The experiment was carried out in the Laboratory of Physical and Physiological Function, Department of Physical Education in Hebei Normal University from April to June in 2006. Twenty-four students were recruited to exercise incrementally in ergometer; in addition, thirty SD rats were assigned to swim incrementally, 15 rats in each group. First, the intensities of metabolic transition were determined, then the exercise protocol was repeated on the conditions of inhaling and not inhaling oxygen. For student group, 50 W loading was incremented every 2 minutes, while the rats were added with 1% of their weights until unacceptable. Gradually incremented loading was used to transform the aerobic mechanism to anaerobic mechanism. The vein blood oxygen partial pressure, pyruvate and lactate contents were measured before and during the exercise (lactate threshold intensity) to evidence the reliability and validity of hypothesis.MAIN OUTCOME MEASURES: The vein blood oxygen partial pressure, pyruvate and lactate contents under lactate threshold intensity and oxygen inhaling supplementary.RESULTS: All 24 testees and 30 rats were involved in the result analysis. ①During the gradually incremented exercise,the lactic acid curve obtained at the end of 2-minute loading showed the difference of metabolic transition intensity and training level in accordance with individual lactic acid threshold, which was obviously lower in the trained exercisers.②Under the lactate threshold intensity, the blood lactate was not correlated to the oxygen partial pressure whether in human body or rats and whether inhaling oxygen or not [(3.61±0.56), (5.43±0.55) mmol/L; (4.46±0.86), (7.80±0.27) kPa,r =0.31, 0.31, P ＞ 0.05]; there was significant difference between the blood lactate and pyruvate contents [(1.04±0.16),(0.91±0.37) mmol/L, P ＜ 0.001]. The human body's saturation of blood oxygen was no less than 98% during the entire protocol. ③Under the repeated exercise and lactate threshold intensity, the pyruvate average value was (0.97±0.17),(1.04±0.16) mmol/L; (0.93±0.25), (0.91 ±0.37) mmol/L, respectively. There was no significant difference between the blood pyruvate before the exercise and under the lactate threshold intensity in both human body and animals (P ＞ 0.05).CONCLUSION: There is no hypoxia at the transition from aerobic to anaerobic metabolism. Oxygen inhaling supplementary has no influence on the mechanism transition; It is not easy for the pyruvate to pass the myocyte membrane, but the lactate can. The result demonstrates that the pyruvate can transform to lactate directly, which can also prevent the accumulation of pyruvate in kytoplasm.
