天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2009年
10期
859-861,后插2
,共4页
乳腺肿瘤%癌%细胞外基质蛋白质类%基质金属蛋白酶类%小鼠%免疫组织化学
乳腺腫瘤%癌%細胞外基質蛋白質類%基質金屬蛋白酶類%小鼠%免疫組織化學
유선종류%암%세포외기질단백질류%기질금속단백매류%소서%면역조직화학
breast neoplasms%carcinoma%extracellular matrix proteins%matrix metalloproteinases%mice immunohistochemistry
目的:探讨细胞外基质金属蛋白酶诱导因子(CD147)及基质金属蛋白酶9(MMP9)在乳腺癌BCML-TAII 99模型移植瘤中的表达及其与肿瘤生长和转移的关系.方法:建立30只小鼠肺转移性乳腺癌BCML-TAII 99模型.用免疫组织化学SP法对小鼠肿瘤组织标本CD147及MMP9蛋白表达进行检测,并分析其与肿瘤大小及转移之间的关系.结果:CD147及MMP9蛋白表达阳性率分别为63.33%(19/30)和53.33%(16/30), CD147、MMP9均阳性表达者13只;两者均阴性者8只,两者表达呈正相关( χ~2 = 48.492,r = 0.786, P ＝ 0.025).CD147、MMP9在转移组与非转移组表达阳性率分别为92.31%、41.18%和76.92%、35.29%,差异有统计学意义(χ~2 = 6.238,P = 0.013和χ~2 = 5.129,P = 0.024).CD147和MMP9蛋白在≥3 g组和＜3 g组的阳性表达率分别为88.89%、25.00%和66.67%、33.33%,两者表达与肿瘤大小有关( χ~2 = 8.452,P = 0.009和χ2 = 4.652,P = 0.039, r分别为0.469和0.366 ).结论:CD147可能通过诱导MMP-9蛋白的产生在乳腺癌的浸润和转移过程中起重要作用.
目的:探討細胞外基質金屬蛋白酶誘導因子(CD147)及基質金屬蛋白酶9(MMP9)在乳腺癌BCML-TAII 99模型移植瘤中的錶達及其與腫瘤生長和轉移的關繫.方法:建立30隻小鼠肺轉移性乳腺癌BCML-TAII 99模型.用免疫組織化學SP法對小鼠腫瘤組織標本CD147及MMP9蛋白錶達進行檢測,併分析其與腫瘤大小及轉移之間的關繫.結果:CD147及MMP9蛋白錶達暘性率分彆為63.33%(19/30)和53.33%(16/30), CD147、MMP9均暘性錶達者13隻;兩者均陰性者8隻,兩者錶達呈正相關( χ~2 = 48.492,r = 0.786, P ＝ 0.025).CD147、MMP9在轉移組與非轉移組錶達暘性率分彆為92.31%、41.18%和76.92%、35.29%,差異有統計學意義(χ~2 = 6.238,P = 0.013和χ~2 = 5.129,P = 0.024).CD147和MMP9蛋白在≥3 g組和＜3 g組的暘性錶達率分彆為88.89%、25.00%和66.67%、33.33%,兩者錶達與腫瘤大小有關( χ~2 = 8.452,P = 0.009和χ2 = 4.652,P = 0.039, r分彆為0.469和0.366 ).結論:CD147可能通過誘導MMP-9蛋白的產生在乳腺癌的浸潤和轉移過程中起重要作用.
목적:탐토세포외기질금속단백매유도인자(CD147)급기질금속단백매9(MMP9)재유선암BCML-TAII 99모형이식류중적표체급기여종류생장화전이적관계.방법:건립30지소서폐전이성유선암BCML-TAII 99모형.용면역조직화학SP법대소서종류조직표본CD147급MMP9단백표체진행검측,병분석기여종류대소급전이지간적관계.결과:CD147급MMP9단백표체양성솔분별위63.33%(19/30)화53.33%(16/30), CD147、MMP9균양성표체자13지;량자균음성자8지,량자표체정정상관( χ~2 = 48.492,r = 0.786, P ＝ 0.025).CD147、MMP9재전이조여비전이조표체양성솔분별위92.31%、41.18%화76.92%、35.29%,차이유통계학의의(χ~2 = 6.238,P = 0.013화χ~2 = 5.129,P = 0.024).CD147화MMP9단백재≥3 g조화＜3 g조적양성표체솔분별위88.89%、25.00%화66.67%、33.33%,량자표체여종류대소유관( χ~2 = 8.452,P = 0.009화χ2 = 4.652,P = 0.039, r분별위0.469화0.366 ).결론:CD147가능통과유도MMP-9단백적산생재유선암적침윤화전이과정중기중요작용.
Objective: To study the expressions of CD147and MMP9 in the lung metastasis model of breast cancer in BCML-TAII99 mice and their correlation with tumor growth and metastasis thereof. Methods: The breast cancer model with lung metastasis was established in BCML-TAII99 mice. The immunohistochemical staining method(SP method) was used to analyze the expressions of CD147 and MMP9 in mice lung metastasis tissue. The relationship between the tumor growth and the metastasis was analyzed. Results: The positive rates of CD147 and MMP9 were 63.33%（19/30）and 53.33%（16/30）respectively in mouse models. There was significant difference in positive rate of expressions between the metastasis group and the non-metastasis group（χ~2= 6.238， P ＝ 0．013； χ~2＝ ＝5．129， P ＝ 0．024). There was a statistically significant positive correlation between the expression of CD147 and MMP9（r = 0．786， P ＝ 0.025). Conclusion: The expression of CD147 may play a crucial role in infiltration and metastasis of breast cancer by inducing the expression of MMP9.
