华侨大学学报（自然科学版）
華僑大學學報（自然科學版）
화교대학학보（자연과학판）
JOURNAL OF HUAQIAO UNIVERSITY(NATURAL SCIENCE)
2010年
1期
37-40
,共4页
量子点%荧光探针%乙酰胆碱酯酶%自组装膜%有机磷农药
量子點%熒光探針%乙酰膽堿酯酶%自組裝膜%有機燐農藥
양자점%형광탐침%을선담감지매%자조장막%유궤린농약
quantum dots%fluorescent probe%acetylcholinesterase%self-assembled multilayers films%organophosphorus pesticide
采用CdSe量子点作为荧光探针,将巯基丙酸稳定的CdSe、聚二烯丙基二甲基氯化铵(PDDA)和乙酰胆碱酯酶(ACHE) 通过静电吸引法,层层组装到石英表面,构建一种荧光性自组装多层膜Quartz/PDDA/CdSe/PDDA/ACHE/PDDA.利用有机磷农药对乙酰胆碱酯酶的抑制机理,成功地将自组装膜用于痕量久效磷农药的检测.实验表明,在乙酰胆碱酯酶被抑制16 min,底物乙酰胆碱浓度为8.8 mmol·L~(-1)的条件下,该膜对久效磷的检测限低至48.64 nmol·L~(-1).所构建的自组装膜稳定性较好、灵敏度较高,可以再生使用.
採用CdSe量子點作為熒光探針,將巰基丙痠穩定的CdSe、聚二烯丙基二甲基氯化銨(PDDA)和乙酰膽堿酯酶(ACHE) 通過靜電吸引法,層層組裝到石英錶麵,構建一種熒光性自組裝多層膜Quartz/PDDA/CdSe/PDDA/ACHE/PDDA.利用有機燐農藥對乙酰膽堿酯酶的抑製機理,成功地將自組裝膜用于痕量久效燐農藥的檢測.實驗錶明,在乙酰膽堿酯酶被抑製16 min,底物乙酰膽堿濃度為8.8 mmol·L~(-1)的條件下,該膜對久效燐的檢測限低至48.64 nmol·L~(-1).所構建的自組裝膜穩定性較好、靈敏度較高,可以再生使用.
채용CdSe양자점작위형광탐침,장구기병산은정적CdSe、취이희병기이갑기록화안(PDDA)화을선담감지매(ACHE) 통과정전흡인법,층층조장도석영표면,구건일충형광성자조장다층막Quartz/PDDA/CdSe/PDDA/ACHE/PDDA.이용유궤린농약대을선담감지매적억제궤리,성공지장자조장막용우흔량구효린농약적검측.실험표명,재을선담감지매피억제16 min,저물을선담감농도위8.8 mmol·L~(-1)적조건하,해막대구효린적검측한저지48.64 nmol·L~(-1).소구건적자조장막은정성교호、령민도교고,가이재생사용.
A fluorescent self-assembled multilayer films (Quartz/PDDA/CdSe/PDDA/ACHE/PDDA) was constructed by putting3-mercaptopropionic acid modified CdSe quantum dots (QDs), PDDA and acetylcholinesterase (ACHE) onto the quartz substrate alternately with the layer by layer assembly technique. And CdSe QDs on the films was used as fluorescent probes. By the enzymatic inhibition, the self-assembled multilayer films could be used to detect trace organophosphorus pesticides successfully. This method was used to detect monocrotophos as low as 48.64 nmol·L~(-1) under the operate conditions that, inhibition time was 16 minutes, substrate concentration was 8.8 mmol·L~(-1) and the pH of substrate solution was about 9.0. The as-prepared multilayer films exhibited good regeneration, stability, and high sensitivity.
