华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2009年
6期
174-177
,共4页
小麦%SSR%分子标记
小麥%SSR%分子標記
소맥%SSR%분자표기
Wheat%SSR%Molecular marker
以小麦基因组DNA为试验材料,探索SSR扩增反应中5种反应组分(模板DNA、dNTPs、引物、Mg~(2+)和Taq DNA聚合酶)对SSR扩增结果的影响.研究发现,引物、Mg~(2+)和Taq DNA聚合酶对PCR扩增结果的影响最显著,其次是模板DNA,dNTP对PCR扩增结果的影响不明显.此外,借助该优化的SSR反应体系,对小麦7B染色体上的多对SSR引物进行了多态性筛选.试验结果表明,优化的SSR扩增体系结合高浓度琼脂糖凝胶能够对显性或差异显著的共显性标记进行高效分离.
以小麥基因組DNA為試驗材料,探索SSR擴增反應中5種反應組分(模闆DNA、dNTPs、引物、Mg~(2+)和Taq DNA聚閤酶)對SSR擴增結果的影響.研究髮現,引物、Mg~(2+)和Taq DNA聚閤酶對PCR擴增結果的影響最顯著,其次是模闆DNA,dNTP對PCR擴增結果的影響不明顯.此外,藉助該優化的SSR反應體繫,對小麥7B染色體上的多對SSR引物進行瞭多態性篩選.試驗結果錶明,優化的SSR擴增體繫結閤高濃度瓊脂糖凝膠能夠對顯性或差異顯著的共顯性標記進行高效分離.
이소맥기인조DNA위시험재료,탐색SSR확증반응중5충반응조분(모판DNA、dNTPs、인물、Mg~(2+)화Taq DNA취합매)대SSR확증결과적영향.연구발현,인물、Mg~(2+)화Taq DNA취합매대PCR확증결과적영향최현저,기차시모판DNA,dNTP대PCR확증결과적영향불명현.차외,차조해우화적SSR반응체계,대소맥7B염색체상적다대SSR인물진행료다태성사선.시험결과표명,우화적SSR확증체계결합고농도경지당응효능구대현성혹차이현저적공현성표기진행고효분리.
In this study,effect of five reaction components,including template DNA,dNTPs,primer,Mg~(2+) and Taq DNA polymerase,on the SSR amplification results were studied using wheat genomic DNA as PCR template.Mg~(2+) and Taq DNA polymerase showing most significant influence on PCR products,and then is template DNA,dNTPs have no significant effect on it.Furthermore,SSR markers on wheat chromosome 7B were screened by the optimized SSR system.Results indicated that,dominant SSR markers or codominant markers with significant difference on length can be separated by optimized SSR amplification system and high concentration agarose gel effectively.
