CAJ | 학술논문

目的探讨补充不同剂量大豆异黄酮对前列腺增生大鼠细胞凋亡与增殖的影响.方法应用丙酸睾酮诱导大鼠前列腺增生,对正常对照组、模型组、低剂量(60 mg·kg~(-1)·d~(-1))、中剂量(120 mg·kg~(-1)·d~(-1))及高剂量(240 mg·kg~(-1)·d~(-1))大豆异黄酮组采用免疫组化和原位末端标记技术方法检测bcl-2、bax、增殖细胞核抗原(PCNA)和细胞凋亡.结果低、中、高剂量大豆异黄酮组大鼠前列腺湿重及前列腺指数均显著低于模型组;中剂量大豆异黄酮组大鼠前列腺湿重及前列腺指数均显著低于低剂量组,与正常对照组无显著性差异.与模型组相比,中、高剂量大豆异黄酮组大鼠bcl-2表达显著降低,bax表达升高,细胞增殖指数显著性降低,而细胞凋亡指数显著性升高,尤以中剂量组效果最为明显.结论前列腺增生大鼠前列腺组织bcl-2和bax的表达调控失衡,大豆异黄酮可能通过调节前列腺组织凋亡基因与抗凋亡基因的表达而抑制前列腺增生.
목적 탐토보충불동제량대두이황동대전렬선증생대서세포조망여증식적영향.방법 응용병산고동유도대서전렬선증생,대정상대조조、모형조、저제량(60 mg·kg~(-1)·d~(-1))、중제량(120 mg·kg~(-1)·d~(-1))급고제량(240 mg·kg~(-1)·d~(-1))대두이황동조채용면역조화화원위말단표기기술방법검측bcl-2、bax、증식세포핵항원(PCNA)화세포조망.결과 저、중、고제량대두이황동조대서전렬선습중급전렬선지수균현저저우모형조;중제량대두이황동조대서전렬선습중급전렬선지수균현저저우저제량조,여정상대조조무현저성차이.여모형조상비,중、고제량대두이황동조대서bcl-2표체현저강저,bax표체승고,세포증식지수현저성강저,이세포조망지수현저성승고,우이중제량조효과최위명현.결론 전렬선증생대서전렬선조직bcl-2화bax적표체조공실형,대두이황동가능통과조절전렬선조직조망기인여항조망기인적표체이억제전렬선증생.
Objective To observe the effects of soybean isoflavone on cell apoptosis and proliferation of rats with benign prostatic hyperplasia. Methods Rats with prostatic hyperplasia were induced by subcutaneously injecting testosterone propionate, immunohistochemical analysis and in situ end labeling were used to examine the expression of bax, bcl-2, and PCNA in rat prostate tissue in the control, the model, the low(60 mg·kg~(-1)·d~(-1)), moderate(120 mg·kg~(-1)·d~(-1))and high dose(240 mg·kg~(-1)·d~(-1))of soybean isoflavone groups.Results The prostate wet weight and prostatic index in all dose groups were significantly lower than that of models and the moderate group was the lowest in all dose groups. There were no significant differences between the moderate group and control group. The expression of bcl-2 and cell proliferation index in moderate and high group were significantly lower than those of model group. The expression of bax and apoptosis index in moderate and high group were significantly higher than those of model group. The effect of the moderate group was the most significant in all dose groups. Conclusions The expressions of bcl-2 and bax in prostatic tissue of benign prostatic hyperplasia rats are imbalanced. Soybean isoflavone might inhibit prostatic hyperplasia by regulating the expressions of bcl-2 and bax in rats.