CAJ | 학술논문

目的:观察隔药灸和电针对克罗恩病(Crohn's Disease,CD)肠纤维化大鼠血清透明质酸(Hyaluronic Acid,HA)、Ⅲ型前胶原(Procollagen Ⅲ,PC Ⅲ)、Ⅳ型胶原(Collagen Ⅳ,C Ⅳ)的影响,探讨针灸抗CD大鼠肠纤维化的作用与机制.方法:SD大鼠随机分为正常组、模型组、隔药灸组、电针组和药物组.采用三硝基苯磺酸制备大鼠CD模型,应用Masson染色光镜下观察结肠胶原纤维增生情况,采用放射免疫分析技术检测大鼠血清HA、PC Ⅲ、CⅣ含量.结果:与正常组比较,模型组大鼠结肠组织胶原纤维增生,血清HA、PCⅢ、CⅣ含量增加;与模型组比较,隔药灸组、电针组及西药组胶原纤维表达减少,血清中HA、PCⅢ、CⅣ含量降低,与电针组、西药组比较,隔药灸组胶原纤维表达减少,血清CⅣ含量降低.结论:隔药灸和电针均可改善CD大鼠肠纤维化病理状态,降低血清HA、PCⅢ、CⅣ含量.
목적:관찰격약구화전침대극라은병(Crohn's Disease,CD)장섬유화대서혈청투명질산(Hyaluronic Acid,HA)、Ⅲ형전효원(Procollagen Ⅲ,PC Ⅲ)、Ⅳ형효원(Collagen Ⅳ,C Ⅳ)적영향,탐토침구항CD대서장섬유화적작용여궤제.방법:SD대서수궤분위정상조、모형조、격약구조、전침조화약물조.채용삼초기분광산제비대서CD모형,응용Masson염색광경하관찰결장효원섬유증생정황,채용방사면역분석기술검측대서혈청HA、PC Ⅲ、CⅣ함량.결과:여정상조비교,모형조대서결장조직효원섬유증생,혈청HA、PCⅢ、CⅣ함량증가;여모형조비교,격약구조、전침조급서약조효원섬유표체감소,혈청중HA、PCⅢ、CⅣ함량강저,여전침조、서약조비교,격약구조효원섬유표체감소,혈청CⅣ함량강저.결론:격약구화전침균가개선CD대서장섬유화병리상태,강저혈청HA、PCⅢ、CⅣ함량.
Objective:To observe the impact of herb-partitioned moxibustion and electroacupuncture on hyaluronic acid(HA),procollagen Ⅲ(PC Ⅲ)and collagen Ⅳ(CⅣ)of intestinal fibrosis in rats with Crohn's disease(CD),and discuss the effect and the mechanism of acupuncture on treating intestinal fibrosis of rats.Methods:SD rats were randomly divided into control group,model group,herb-partitioned moxibustion group,electroacupuncture group and medicine group.The model of CD was developed by trinitrobenzene sulfonic acid.Masson stain was used to observe the collagen fibers hyperplasia in colon and radioimmunoassay was adopted to detect the content of HA,PC Ⅲ and C Ⅳ in serum.Results:Compared with the control group,the proliferation of colonic collagen fibers,as well as the HA,PC Ⅲ and CⅣ in serum increased in the model group.Compared with the model group,the expression of collagen fibers,serum HA,PC Ⅲ and C Ⅳ decreased in the herb-partitioned moxibustion group,electroacupuncture group and medicine group.The expression of collagen fibers serum HA,PC Ⅲ and C Ⅳ in the herb-partitioned moxibustion group were lower than in the electroacupuncture group and medicine group.Conclusion:Herb-partitioned moxibustion and electroacupuncture effectively improved the pathological state of intestinal fibrosis in rats with CD and reduced the content of HA,PC Ⅲ and C Ⅳ in serum.