CAJ | 학술논문

通过拨动细胞实现遗传物质的移动,并在对卵母细胞的拔动机理进行深入分析的基础上,根据极体是否可见将卵母细胞的自动拨动过程分为翻动和转动两部分.进一步的,对翻动过程中的卵母细胞进行建模,并通过实验首次提出并实现了四种卵母细胞的自动拨动轨迹.随后,对影响卵母细胞翻动幅度的因素进行了研究,并通过实验证实翻动的幅度可以通过拨动微针的离焦高度进行控制.最后,绵羊的大批量核移植操作应用结果表明,使用设计的自动拨动轨迹的移动遗传物质的成功率可达95％以上.同时,拨动过程中极体出现的位置也和本文提出的翻动模型计算的结果一致,从而验证了本文工作的有效性.
통과발동세포실현유전물질적이동,병재대란모세포적발동궤리진행심입분석적기출상,근거겁체시부가견장란모세포적자동발동과정분위번동화전동량부분.진일보적,대번동과정중적란모세포진행건모,병통과실험수차제출병실현료사충란모세포적자동발동궤적.수후,대영향란모세포번동폭도적인소진행료연구,병통과실험증실번동적폭도가이통과발동미침적리초고도진행공제.최후,면양적대비량핵이식조작응용결과표명,사용설계적자동발동궤적적이동유전물질적성공솔가체95％이상.동시,발동과정중겁체출현적위치야화본문제출적번동모형계산적결과일치,종이험증료본문공작적유효성.
The mechanism of oocyte's movement is analyzed which can he used to move hereditary substance.This process is divided into revolving course and rotation course according to the availability of the polar body's information.Then a model of the oocyte in revolving course is propxsed and four kinds of moving trajectories of the injector are realized for the first time in this area.After that,the factors that influence the revolving range of oocyte are studied deeply.Furthermore,a problem of revolving range control is considered by defocusing distance.In the end,application results are reported and they demonstrate that the success rate of autonomous mvement of oocyte can reach more than 95 ％.As can be seen that the appearing positions of polar bodies in experiments agree well with modeling results,which verifies the validity of the work.