国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2008年
3期
118-123
,共6页
刘俊琴%申丽洁%马鸣旺%李伟
劉俊琴%申麗潔%馬鳴旺%李偉
류준금%신려길%마명왕%리위
旋毛虫%ELISA%血清%唾液
鏇毛蟲%ELISA%血清%唾液
선모충%ELISA%혈청%타액
Trichinella spiralis%ELIsA%Serum%Saliva
目的 建立检测血清和唾液中旋毛虫抗体的酶联免疫吸附试验(ELISA)方法.方法 应用方阵滴定法,筛选适宜的旋毛虫抗原(肌肉幼虫可溶性抗原、肌肉幼虫排泄分泌抗原、成虫可溶性抗原、成虫排泄分泌抗原)浓度、血清和唾液稀释度、辣根过氧化物酶标记的山羊抗兔、山羊抗人IgG抗体稀释度.共有20份旋毛虫感染兔、10份旋毛虫病患者血清和唾液用于这4种抗原的敏感性试验,20份健康兔与38份其他寄生虫感染兔和患者的血清和唾液用于这4种抗原的特异性试验.结果 这4种抗原适宜包被浓度依次为8.0 μg/ml、6.0 μg/ml、10.0 μg/ml、9.0 μg/ml.适宜的血清稀释度依次为1:100、1:200、1:50、1:200,唾液均用原液.适宜的山羊抗兔、山羊抗人IgG稀释度分别为1:2 500和l:2 000.这4种抗原检测旋毛虫感染兔血清和唾液的敏感性分别为100%和80%~100%,检测旋毛虫病患者血清和唾液的敏感性分别为100%和60%~80%;检测血清和唾液的特异性依次为81.03%、89.65%、77.59%、82.76%和93.10%、96.55%、89.65%、91.35%.结论 建立了检测兔和人血清及唾液中旋毛虫lgG抗体的ELISA方法.当采集血清标本有困难时,可将唾液替代血清用于检测旋毛虫病.
目的 建立檢測血清和唾液中鏇毛蟲抗體的酶聯免疫吸附試驗(ELISA)方法.方法 應用方陣滴定法,篩選適宜的鏇毛蟲抗原(肌肉幼蟲可溶性抗原、肌肉幼蟲排洩分泌抗原、成蟲可溶性抗原、成蟲排洩分泌抗原)濃度、血清和唾液稀釋度、辣根過氧化物酶標記的山羊抗兔、山羊抗人IgG抗體稀釋度.共有20份鏇毛蟲感染兔、10份鏇毛蟲病患者血清和唾液用于這4種抗原的敏感性試驗,20份健康兔與38份其他寄生蟲感染兔和患者的血清和唾液用于這4種抗原的特異性試驗.結果 這4種抗原適宜包被濃度依次為8.0 μg/ml、6.0 μg/ml、10.0 μg/ml、9.0 μg/ml.適宜的血清稀釋度依次為1:100、1:200、1:50、1:200,唾液均用原液.適宜的山羊抗兔、山羊抗人IgG稀釋度分彆為1:2 500和l:2 000.這4種抗原檢測鏇毛蟲感染兔血清和唾液的敏感性分彆為100%和80%~100%,檢測鏇毛蟲病患者血清和唾液的敏感性分彆為100%和60%~80%;檢測血清和唾液的特異性依次為81.03%、89.65%、77.59%、82.76%和93.10%、96.55%、89.65%、91.35%.結論 建立瞭檢測兔和人血清及唾液中鏇毛蟲lgG抗體的ELISA方法.噹採集血清標本有睏難時,可將唾液替代血清用于檢測鏇毛蟲病.
목적 건립검측혈청화타액중선모충항체적매련면역흡부시험(ELISA)방법.방법 응용방진적정법,사선괄의적선모충항원(기육유충가용성항원、기육유충배설분비항원、성충가용성항원、성충배설분비항원)농도、혈청화타액희석도、랄근과양화물매표기적산양항토、산양항인IgG항체희석도.공유20빈선모충감염토、10빈선모충병환자혈청화타액용우저4충항원적민감성시험,20빈건강토여38빈기타기생충감염토화환자적혈청화타액용우저4충항원적특이성시험.결과 저4충항원괄의포피농도의차위8.0 μg/ml、6.0 μg/ml、10.0 μg/ml、9.0 μg/ml.괄의적혈청희석도의차위1:100、1:200、1:50、1:200,타액균용원액.괄의적산양항토、산양항인IgG희석도분별위1:2 500화l:2 000.저4충항원검측선모충감염토혈청화타액적민감성분별위100%화80%~100%,검측선모충병환자혈청화타액적민감성분별위100%화60%~80%;검측혈청화타액적특이성의차위81.03%、89.65%、77.59%、82.76%화93.10%、96.55%、89.65%、91.35%.결론 건립료검측토화인혈청급타액중선모충lgG항체적ELISA방법.당채집혈청표본유곤난시,가장타액체대혈청용우검측선모충병.
Objective To establish the ELISA method for detection of anti-Trichinella antibody IgG in sera and saliva. Methods The phalanx titration was used for the selection of the ELISA experimental conditions such as the optimal coating concentrations of T. spiralis antigens including muscle larval soluble antigen (MLSA),muscle larval excretory-secretory antigen (MLESA),adult wornl soluble antigen (AWSA),adult worm excretory-secretory antigen (AWESA), the dilutions of sera and saliva, the dilutions of the goat anti-rabbit and the goat anti-human IgG conjugated with horseradish peroxidase (HRP).Sera and saliva from 20 rabbits,10 patients infected with T. spiralis were used for the sensitivity assay of the 4 antigens.while sera and saliva from 20 healthy parasite-free rabbits and persons,sera and saliva from 38 rabbits and patients infected with other parasites were detected for the specificity of the 4 antigens.Results The optimal coating concentrations of the 4 antigens were 8.0 μg/ml,6.0 μg/ml,10.0 μg/ml,9.0 μg/ml,respectively.The optimal dilutions of sera were 1:100,1:200,1:50,1:200 respectively.while saliva was used without dilution.The dilutions of the goat anti.rabbit and the goat anti-human IgG were 1:2 500 and 1:2 000.respectively.The sensitivities of the 4 antigens for detecting the sera and saliva from rabbits infected with T. spiralis were 100% and 80%-100%.respectively.The sensitivities of the 4 antigens for detecting the sera and saliva from patients with trichinellosis were 100% and 60%-80%.respectively.The specificities of the 4 antigens for detecting the sera and saliva were 81.03%,89.65%,77.59%.82.76%and 93.10%,96.55%,89.65%.91.35%respectively. Conclusion The ELISA assays for detection of anti-T.spiralis IgG antibody in serum and saliva samples were established.Saliva samples were promising in substituting serum for detection of triehinellosis when there were diffieulties to collect serum samples.
