CAJ | 학술논문

目的探讨丹参提取物764-3对高氧暴露所致新生大鼠急性肺损伤的保护作用,并将其作用与超氧化物歧化酶(SOD)进行比较.方法采用新生Sprague Dawley大鼠置于≥90%O2中7 d以建立高氧急性肺损伤模型,分别用SCD和764-3进行干预,对比肺系数、丙二醛(MDA)含量、SOD活性、辐射状肺泡计数(RAC)和病理形态学变化,并采用脱氧核糖核酸转移酶介导的细胞凋亡标记技术法(TUNEL)检测肺细胞凋亡情况.结果 SOD干预组和764-3干预组在高氧暴露7 d时的MDA含量和凋亡细胞数均低于高氧对照组(P<0.05或P<0.01),病理形态学改变要轻于高氧对照组,RAC及SOD活性则要高于高氧对照组(P<0.05或P<0.01),且两种抗氧化剂作用近似(P>0.05).但764-3干预组的肺水肿与高氧对照组差异无显著性(P>0.05).结论 764-3对新生大鼠高氧所致的急性肺损伤有一定的保护作用.
목적 탐토단삼제취물764-3대고양폭로소치신생대서급성폐손상적보호작용,병장기작용여초양화물기화매(SOD)진행비교.방법 채용신생Sprague Dawley대서치우≥90%O2중7 d이건립고양급성폐손상모형,분별용SCD화764-3진행간예,대비폐계수、병이철(MDA)함량、SOD활성、복사상폐포계수(RAC)화병리형태학변화,병채용탈양핵당핵산전이매개도적세포조망표기기술법(TUNEL)검측폐세포조망정황.결과 SOD간예조화764-3간예조재고양폭로7 d시적MDA함량화조망세포수균저우고양대조조(P<0.05혹P<0.01),병리형태학개변요경우고양대조조,RAC급SOD활성칙요고우고양대조조(P<0.05혹P<0.01),차량충항양화제작용근사(P>0.05).단764-3간예조적폐수종여고양대조조차이무현저성(P>0.05).결론 764-3대신생대서고양소치적급성폐손상유일정적보호작용.
Objective To study the protective effect of Radix Salviae Milliorrhizae Extract (764-3) on hyperoxia-induced acute lung injury of neonatal rats and to compare the effect of it with that of superoxide dismutase (SOD).Methods Hyperoxia lung injury model was established by putting Sprague Dawley neonatal rats into self-made oxygen box within high-concentration oxygen(≥90 % O2)for 7 days. Interfered with SOD and 764-3 respectively, the lung index, contents of malondialdehyde (MDA), activity of SOD, radical alveolar counts (RAC) and pathological changes have been compared. Meanwhile, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was utilized to detect the number of apoptosis of lung cells. Results On the seventh day, the contents of MDA and the number of apoptosis of lung cells of SOD prevention group and 764-3 prevention group were lower than those of the hyperoxia-exposed group( P < 0.05 or P < 0.01 ), while RAC and activity of SOD were higher(P < 0.05 or P < 0.01) ; pathological changes of apoptosis of lung cells of SOD prevention group and 764-3 prevention group were less than those of the hyperoxia-exposed group. The protective effect of SOD and 764-3 were similar(P >0.05).However, compared with those of hyperoxia-exposed group, the lung index of 764-3 prevention group showed insignificant difference(P >0.05). Conclusion Radix Salviae Milliorrhizae Extract (764-3) may have certain protective effect on hyperoxia-in-duced acute lung injury.