中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
8期
473-479
,共7页
王琳%谢青%龚邦东%安宝燕%徐玉敏%蔡伟%王晖%周惠娟%郭斯敏%俞红%郭清
王琳%謝青%龔邦東%安寶燕%徐玉敏%蔡偉%王暉%週惠娟%郭斯敏%俞紅%郭清
왕림%사청%공방동%안보연%서옥민%채위%왕휘%주혜연%곽사민%유홍%곽청
肝炎病毒,乙型%微RNAs%细胞因子类%炎症%基因表达%单核细胞%肝炎,乙型
肝炎病毒,乙型%微RNAs%細胞因子類%炎癥%基因錶達%單覈細胞%肝炎,乙型
간염병독,을형%미RNAs%세포인자류%염증%기인표체%단핵세포%간염,을형
Hepatitis B virus%microRNAs%Cytokines%Inflammation%Gene expression%Monocyte%Hepatitis B
目的 探讨乙型肝炎患者外周血单个核细胞(PBMC)中微小RNA(miRNA)和细胞因子的变化及其与炎性反应的相互关系.方法 收集健康对照者17例,急性乙型肝炎发作期、病毒清除期和恢复期患者各20例,慢性乙型肝炎轻度、中度和重度患者各20例和乙型肝炎后肝硬化患者20例.分离PBMC,采用反转录-荧光定量PCR方法检测miRNA146、miRNA155、miRNA181、IFN-α、IFN-β、IFN诱导基因54(ISG54)、IFN调节因子5(IRF5)的表达.多组间比较采用单因素方差分析.结果 急性乙型肝炎患者PBMC中miRNA155的表达水平在急性发作期(2.386±1.835)较高,明显高于健康对照组(1.498±1.276),差异有统计学意义(F=1.137,P=0.045),随疾病进入发作期、病毒清除期(1.633±2.291)、恢复期(0.642±0.836),其表达逐渐降低(F=2.122,P=0.022).同时IFN-α、IFN-β随急性发作期(7.059±9.594、4.767±6.725)、病毒清除期(2.216±2.148、1.750±1.403)和恢复期(0.642±0.836、1.201±0.779)其表达也逐渐降低(F=1.880,P=0.038;F=1.835,P=0.048).相关性分析发现,miRNA155与IFN-α、IFN-β均具有良好的正相关性(r=0.483,P=0.004;r=0.660,P=0.0002).在急性HBV感染患者中,miRNA155的表达与ALT、Tbil均呈正相关(r=0.342,P=0.006;r=0.322,P=0.011),但与血清HBV DNA载量无相关性.miRNA181在HBV感染者PBMC中的表达,除急性乙型肝炎恢复期(1.873±0.998)外,均高于健康对照组(1.307±0.935)(F=2.072,P=0.045),但HBV感染各组间差异无统计学意义.miRNA146的表达水平变化不明显.结论 在HBV感染过程中,miRNAs参与了宿主的抗HBV免疫反应,并与细胞因子表达相关.
目的 探討乙型肝炎患者外週血單箇覈細胞(PBMC)中微小RNA(miRNA)和細胞因子的變化及其與炎性反應的相互關繫.方法 收集健康對照者17例,急性乙型肝炎髮作期、病毒清除期和恢複期患者各20例,慢性乙型肝炎輕度、中度和重度患者各20例和乙型肝炎後肝硬化患者20例.分離PBMC,採用反轉錄-熒光定量PCR方法檢測miRNA146、miRNA155、miRNA181、IFN-α、IFN-β、IFN誘導基因54(ISG54)、IFN調節因子5(IRF5)的錶達.多組間比較採用單因素方差分析.結果 急性乙型肝炎患者PBMC中miRNA155的錶達水平在急性髮作期(2.386±1.835)較高,明顯高于健康對照組(1.498±1.276),差異有統計學意義(F=1.137,P=0.045),隨疾病進入髮作期、病毒清除期(1.633±2.291)、恢複期(0.642±0.836),其錶達逐漸降低(F=2.122,P=0.022).同時IFN-α、IFN-β隨急性髮作期(7.059±9.594、4.767±6.725)、病毒清除期(2.216±2.148、1.750±1.403)和恢複期(0.642±0.836、1.201±0.779)其錶達也逐漸降低(F=1.880,P=0.038;F=1.835,P=0.048).相關性分析髮現,miRNA155與IFN-α、IFN-β均具有良好的正相關性(r=0.483,P=0.004;r=0.660,P=0.0002).在急性HBV感染患者中,miRNA155的錶達與ALT、Tbil均呈正相關(r=0.342,P=0.006;r=0.322,P=0.011),但與血清HBV DNA載量無相關性.miRNA181在HBV感染者PBMC中的錶達,除急性乙型肝炎恢複期(1.873±0.998)外,均高于健康對照組(1.307±0.935)(F=2.072,P=0.045),但HBV感染各組間差異無統計學意義.miRNA146的錶達水平變化不明顯.結論 在HBV感染過程中,miRNAs參與瞭宿主的抗HBV免疫反應,併與細胞因子錶達相關.
목적 탐토을형간염환자외주혈단개핵세포(PBMC)중미소RNA(miRNA)화세포인자적변화급기여염성반응적상호관계.방법 수집건강대조자17례,급성을형간염발작기、병독청제기화회복기환자각20례,만성을형간염경도、중도화중도환자각20례화을형간염후간경화환자20례.분리PBMC,채용반전록-형광정량PCR방법검측miRNA146、miRNA155、miRNA181、IFN-α、IFN-β、IFN유도기인54(ISG54)、IFN조절인자5(IRF5)적표체.다조간비교채용단인소방차분석.결과 급성을형간염환자PBMC중miRNA155적표체수평재급성발작기(2.386±1.835)교고,명현고우건강대조조(1.498±1.276),차이유통계학의의(F=1.137,P=0.045),수질병진입발작기、병독청제기(1.633±2.291)、회복기(0.642±0.836),기표체축점강저(F=2.122,P=0.022).동시IFN-α、IFN-β수급성발작기(7.059±9.594、4.767±6.725)、병독청제기(2.216±2.148、1.750±1.403)화회복기(0.642±0.836、1.201±0.779)기표체야축점강저(F=1.880,P=0.038;F=1.835,P=0.048).상관성분석발현,miRNA155여IFN-α、IFN-β균구유량호적정상관성(r=0.483,P=0.004;r=0.660,P=0.0002).재급성HBV감염환자중,miRNA155적표체여ALT、Tbil균정정상관(r=0.342,P=0.006;r=0.322,P=0.011),단여혈청HBV DNA재량무상관성.miRNA181재HBV감염자PBMC중적표체,제급성을형간염회복기(1.873±0.998)외,균고우건강대조조(1.307±0.935)(F=2.072,P=0.045),단HBV감염각조간차이무통계학의의.miRNA146적표체수평변화불명현.결론 재HBV감염과정중,miRNAs삼여료숙주적항HBV면역반응,병여세포인자표체상관.
Objective To investigate the expressions of microRNAs (miRNA) and cytokines in the peripheral blood mononuclear cells (PBMCs) of patients with hepatitis B virus (HBV) infection and analyze their relationship with inflammation. Methods PBMCs were collected from acute hapatitis B (AHB) patients of 3 groups, including acute episode, virus clearance, recover period, and chronic hepatitis B (CHB) of mild, medium, severe type, and HBV-related liver cirrhosis. Each group included 20 patients, and 17 healthy donors were as control. Reverse transcription-polymerase chain reaction was used to measure miRNA146, miRNA155, miRNA181, interferon (IFN)-α, IFN-β,interferon induced gene 54 (ISG54) and interferon regulate factor 5 (IRF5). Comparisons among groups were done by one factor analysis of variance. Results The expression of miRNA155 was high in acute episode of AHB (2. 386± 1. 835), and higher than healthy control (1. 498± 1. 276) (F=1. 137,P-=0. 045), while reduced in acute episode, virus clearance (1. 633±2. 291), and recover period (0. 642±0. 836) (F=2. 122,P=0. 022). The expressions of IFN-α and IFN-β in AHB reduced in acute episode, virus clearance and recover period ( F = 1. 880, P = 0. 038 ; F= 1. 835, P = 0. 048).The expression of miRNA155 in AHB is closely correlated with IFN-α and IFN-β (r = 0. 483, P=0. 004; r= 0. 660, P= 0. 0002, respectively). In addition, in HBV infectious patients, the expression of miRNA155 was correlated with alanine transarninase (ALT), serum bilirubin (TBil) (r=0. 342,P=0. 006; r=0. 322, P= 0. 011, respectively), but not with HBV DNA load. Compared with healthy control (1. 307+ 0. 935), miRNA181 was higher in patients with HBV infection (F= 2. 072, P=0. 045) except AHB in recover period (1. 873±0. 998). There was no statistical difference in the miRNA146 expression between various groups. Conclusions The exprossion of miRNAs might be involved in the host anti-HBV immune response during HBV infection, and closely correlated with expression of IFN-related immune factors.
