CAJ | 학술논문

目的探讨蝮蛇毒介导的小鼠急性肝损伤肝脏病理变化、血清丙氨酸氨基转移酶(ALT)及血清中P-选择素(P-selectin)水平的变化,与不同蝮蛇毒剂量、中毒时间的关系.方法将72只清洁级昆明雄性小鼠采用两因素析因设计随机(随机数字法)分成9组,每组8只小鼠,蝮蛇毒设三水平:零剂量(0.0 mg/kg)、低剂量(0.5 mg/kg)、高剂量(1.0mg/kg)；中毒时间设3水平:3、8、24 h.采集小鼠血清及肝脏标本,观察肝脏病理变化、ALT、P-选择素表达.结果肌注蝮蛇毒的实验组小鼠肝脏病理积分、血清ALT、血清P-选择素值明显高于肌注生理盐水的对照组(P＜0.05)；高剂量组高于低剂量组(P ＜0.05)；8h组高于3h组(P＜0.05)；24 h组高于8h组(P＜0.05)；随着蝮蛇毒剂量的增加、中毒时间的延长病理积分越高,血清ALT值越高,血清P-选择素含量越高.血清中P-选择素表达水平与肝脏病变积分相关性分析,两者呈正相关(r=0.98,P＜0.05).结论蝮蛇毒可以导致小鼠急性肝损伤,ALT值升高,P-选择素表达增高,呈一定的时间、剂量效应趋势.蝮蛇毒导致小鼠急性肝脏损伤与P-选择素表达增高相关,提示血清中P-选择素表达增高可能是蝮蛇毒导致小鼠急性肝脏损伤的机制之一.
목적 탐토복사독개도적소서급성간손상간장병리변화、혈청병안산안기전이매(ALT)급혈청중P-선택소(P-selectin)수평적변화,여불동복사독제량、중독시간적관계.방법 장72지청길급곤명웅성소서채용량인소석인설계수궤(수궤수자법)분성9조,매조8지소서,복사독설삼수평:령제량(0.0 mg/kg)、저제량(0.5 mg/kg)、고제량(1.0mg/kg)；중독시간설3수평:3、8、24 h.채집소서혈청급간장표본,관찰간장병리변화、ALT、P-선택소표체.결과 기주복사독적실험조소서간장병리적분、혈청ALT、혈청P-선택소치명현고우기주생리염수적대조조(P＜0.05)；고제량조고우저제량조(P ＜0.05)；8h조고우3h조(P＜0.05)；24 h조고우8h조(P＜0.05)；수착복사독제량적증가、중독시간적연장병리적분월고,혈청ALT치월고,혈청P-선택소함량월고.혈청중P-선택소표체수평여간장병변적분상관성분석,량자정정상관(r=0.98,P＜0.05).결론 복사독가이도치소서급성간손상,ALT치승고,P-선택소표체증고,정일정적시간、제량효응추세.복사독도치소서급성간장손상여P-선택소표체증고상관,제시혈청중P-선택소표체증고가능시복사독도치소서급성간장손상적궤제지일.
Objective To explore the relationship between pathological changes of liver,serum ALT,expression of P-selectin in serum of mice and different dose of venom,the poisoning time induced by Agkistrodon Halys Pallas venom.Methods Using two -factor factorial design,72 male Kunming mice of clean grade were randomly ( random number) divided into 9 groups (n =8).As per the different doses of snake venom injected and different lengths of time after venom injected,the 9 groups were 0-dose (0.0 mg/kg) 3 hours after saline given group,0-dose 8 hours after saline given group,0-dose 24 hours after saline given group,low-dose (0.5 mg/kg) 3 hours after venom given group,low-dose 8 hours after venom given group,low-dose 24 hours after venom given,high-dose ( 1.0 mg/kg) 3 hours after venom given group,high-dose 8 hours after venom given group and high-dose 24 hours after venom given group.The pathological change of liver tissue was determined,and ALT and expression of P-selectin in serum were detected.Results In the venom given groups,the liver histopathologic scores,serum ALT value and expression of Pselectin in serum were significantly higher than those in control group ( P ＜ 0.05 ),and those in high-dose group were higher than those in low-dose group (P ＜ 0.05 ),and those in 8h groups were higher than those in 3h groups (P＜0.05),and those in 24 h groups were higher than those in 8 h groups (P ＜0.05).As the dose of venom increased and the length of time after venom given extended,the liver histopathologic scores,serum ALT and expression of P-selectin in serum were higher.A positive correlation between Pselectin expression and liver histopathologic scores was found after correlation analysis ( r =0.98,P ＜0.05 ).Conclusions Agkistrodon snake venom can cause acute liver injury with elevated ALT and Pselectin in mice.As higher dose of venom given and longer length of time after venom given extended,more serum ALT and P-selectin are produced.Between acute liver injury and high expression of P-selectin exists a correlation,suggesting overexpression of P-selectin is one of the mechanisms of acute liver injury induced by Agkistrodon Halys Pallas venom.