中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2011年
8期
734-737
,共4页
糖尿病%当归补血汤%玻璃体%血清%血管内皮生长因子
糖尿病%噹歸補血湯%玻璃體%血清%血管內皮生長因子
당뇨병%당귀보혈탕%파리체%혈청%혈관내피생장인자
Diabetic mellitus%Angelicae sinensis decoction for supplementing blood%Vitreous%Serum%Vascular endothelial growth factor
背景 糖尿病视网膜病变(DR)主要的病理改变是新生血管生成,血管内皮生长因子(VEGF)是诱导新生血管生成的重要因子,因此是评价糖尿病(DM)血管性疾病并发症的重要指标.目的 研究当归补血汤(ASD)对链脲佐菌素(STZ)诱导的DM大鼠玻璃体和血清中VEGF表达的影响,探讨其在DR治疗中的作用机制.方法 雄性SPF级SD大鼠34只以高脂饮食喂养8周,然后腹腔注射小剂量(25 mg/kg)STZ建立2型DM大鼠模型,将血糖＞13.9 mmol/L者纳入实验并用计算机数字随机生成法随机分组.11只DM动物作为模型组,ASD治疗组10只、格列奇特治疗组(Gli组)10只,于造模第4天开始分别以3.6g/kg ASD、10 mg/kg Gli(5 ml)和饮用水灌胃至实验结束.另取10只正常匹配动物饲以正常饲料作为正常对照组,以等量饮用水灌胃,分别于灌胃后第4、第8周收集各组动物腹主动脉血检测血糖、血甘油三酯(TG)、高密度脂蛋白(HDL),灌胃后第8周采用酶联免疫吸附试验(ELISA)测定大鼠血清和玻璃体中VEGF的质量浓度.结果 灌胃治疗后第4周、第8周,ASD组血糖和TG明显低于DM组,差异有统计学意义(P＜0.05);HDL高于DM组,差异有统计学意义(P＜0.05);灌胃后第8周,正常对照组、ASD组、Gli组间血清、玻璃体中VEGF质量浓度的差异均无统计学意义(P＞0.05);而DM组大鼠血清中VEGF质量浓度明显低于正常对照组,玻璃体中VEGF质量浓度明显高于正常对照组,差异均有统计学意义(P＜0.01);DM组大鼠血清中和玻璃体中VEGF质量浓度分别为(147.65±4.55)和(673.45±100.85)ng/L,二者间无明显直线相关关系(r=0.314,P＞0.05).结论 结果提示ASD能够降低玻璃体中VEGF的质量浓度,可能有延缓DM大鼠DR的发生的作用.
揹景 糖尿病視網膜病變(DR)主要的病理改變是新生血管生成,血管內皮生長因子(VEGF)是誘導新生血管生成的重要因子,因此是評價糖尿病(DM)血管性疾病併髮癥的重要指標.目的 研究噹歸補血湯(ASD)對鏈脲佐菌素(STZ)誘導的DM大鼠玻璃體和血清中VEGF錶達的影響,探討其在DR治療中的作用機製.方法 雄性SPF級SD大鼠34隻以高脂飲食餵養8週,然後腹腔註射小劑量(25 mg/kg)STZ建立2型DM大鼠模型,將血糖＞13.9 mmol/L者納入實驗併用計算機數字隨機生成法隨機分組.11隻DM動物作為模型組,ASD治療組10隻、格列奇特治療組(Gli組)10隻,于造模第4天開始分彆以3.6g/kg ASD、10 mg/kg Gli(5 ml)和飲用水灌胃至實驗結束.另取10隻正常匹配動物飼以正常飼料作為正常對照組,以等量飲用水灌胃,分彆于灌胃後第4、第8週收集各組動物腹主動脈血檢測血糖、血甘油三酯(TG)、高密度脂蛋白(HDL),灌胃後第8週採用酶聯免疫吸附試驗(ELISA)測定大鼠血清和玻璃體中VEGF的質量濃度.結果 灌胃治療後第4週、第8週,ASD組血糖和TG明顯低于DM組,差異有統計學意義(P＜0.05);HDL高于DM組,差異有統計學意義(P＜0.05);灌胃後第8週,正常對照組、ASD組、Gli組間血清、玻璃體中VEGF質量濃度的差異均無統計學意義(P＞0.05);而DM組大鼠血清中VEGF質量濃度明顯低于正常對照組,玻璃體中VEGF質量濃度明顯高于正常對照組,差異均有統計學意義(P＜0.01);DM組大鼠血清中和玻璃體中VEGF質量濃度分彆為(147.65±4.55)和(673.45±100.85)ng/L,二者間無明顯直線相關關繫(r=0.314,P＞0.05).結論 結果提示ASD能夠降低玻璃體中VEGF的質量濃度,可能有延緩DM大鼠DR的髮生的作用.
배경 당뇨병시망막병변(DR)주요적병리개변시신생혈관생성,혈관내피생장인자(VEGF)시유도신생혈관생성적중요인자,인차시평개당뇨병(DM)혈관성질병병발증적중요지표.목적 연구당귀보혈탕(ASD)대련뇨좌균소(STZ)유도적DM대서파리체화혈청중VEGF표체적영향,탐토기재DR치료중적작용궤제.방법 웅성SPF급SD대서34지이고지음식위양8주,연후복강주사소제량(25 mg/kg)STZ건립2형DM대서모형,장혈당＞13.9 mmol/L자납입실험병용계산궤수자수궤생성법수궤분조.11지DM동물작위모형조,ASD치료조10지、격렬기특치료조(Gli조)10지,우조모제4천개시분별이3.6g/kg ASD、10 mg/kg Gli(5 ml)화음용수관위지실험결속.령취10지정상필배동물사이정상사료작위정상대조조,이등량음용수관위,분별우관위후제4、제8주수집각조동물복주동맥혈검측혈당、혈감유삼지(TG)、고밀도지단백(HDL),관위후제8주채용매련면역흡부시험(ELISA)측정대서혈청화파리체중VEGF적질량농도.결과 관위치료후제4주、제8주,ASD조혈당화TG명현저우DM조,차이유통계학의의(P＜0.05);HDL고우DM조,차이유통계학의의(P＜0.05);관위후제8주,정상대조조、ASD조、Gli조간혈청、파리체중VEGF질량농도적차이균무통계학의의(P＞0.05);이DM조대서혈청중VEGF질량농도명현저우정상대조조,파리체중VEGF질량농도명현고우정상대조조,차이균유통계학의의(P＜0.01);DM조대서혈청중화파리체중VEGF질량농도분별위(147.65±4.55)화(673.45±100.85)ng/L,이자간무명현직선상관관계(r=0.314,P＞0.05).결론 결과제시ASD능구강저파리체중VEGF적질량농도,가능유연완DM대서DR적발생적작용.
Background The main mechanism of diabetic retinopathy (DR) is the formation of retinal neovascularization. Vascular endothelial growth factors (VEGF) is primarily factor for DR. Objective The goal of this study was to investigate the effects of angelicae sinensis decoction for supplementing blood ( ASD), a kind of traditional prescription of Chinese herbal medicine, on vascular endothelial growth factor (VEGF) level in vitreous and serum of STZ-induced type 2 diabetes mellitus. Methods Thirty-four SPF male SD rats were fed using high fat diet for 8 weeks and then the streptozocin of 25 mg/kg was intraperitoneally injected to create the type 2 diabetic models. ASD (3.6 g/kg) and 10 mg/kg Gli of 5 mi were administered intragastrically from the 4th day of high fat die though 8 weeks respectively. Other 10 matched rats were used as normal control group. The body weight, blood glucose level ,triglycerides (TG) and high density lipoprotein (HDL) were detected in 4 and 8 weeks after usage of drug. The vitreous and serum samples were obtained in the 8 th weeks for the VEGF detection by enzyme-linked immunosorbent assay (ELISA). This experiment comlied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results The body weight and HDL in DM group were significantly lower than those of normal control group in 4 or 8 weeks ( P＜0. 01 ), but in ASD and Gli groups ,the body weight and HDL were higher than those of the DM group (P＜0. 05 ). the blood glucose level and TG were elevated in DM group compared with normal control group (P＜0. 01 ), but those in ASD group and Gli group were decreased in comparison with DM group ( P＜0.05 ). EIISA revealed that no significant differences were found in VEGF levels between normal control group and ASD group or Gli group (P＞0. 05 ), but in DM group, the serum VEGF level (147.65±4. 55 ng/L) was reduced and vitreous VEGF level (673.45± 100. 85 ng/L) was raised,showing the considerable differences (P＜ 0.05 ). No any correlation was found between serum VEGF level and vitreous VEGF level (r=0.314,P＞0. 05). Conclusion ASD can decrease the VEGF level in vitreous,implying that ASD postpone the occurrence of diabetic retinopathy in DM rat.
