CAJ | 학술논문

目的观察干细胞介入治疗股骨头缺血性坏死的疗效.方法毕格犬18只,随机分为三组:A组(对照组)、B组(右归饮组)、C组(干细胞介入组)各6只.通过液氮冷冻法,建立毕格犬股骨头缺血性坏死模型.分离、培养并标记骨髓问质干细胞.造模后3周,B组给予右归饮灌胃,A组与C组以蒸馏水灌胃;C组动脉灌注标记后的骨髓间质干细胞(5×106～1×107/ml)1 m1,A组及B组灌注生理盐水.治疗后4周及8周观察股骨头大体、影像学、组织病理学改变,及股骨头供血血管的数量、直径.采用免疫组化染色观察VEGF、Brdu的阳性表达,通过RT-PCR测定VEGF、TGF-β、OPG、RANKL的表达.结果 C组供血血管分支增粗、增多,阻塞血管再通,血管数量增多、直径增大.B组、C组关节软骨、骨小梁的结构和形态较A组改善;免疫组化检测C组VEGF阳性表达率较A组及B组高;RT-PCR检测C组VEGF、TGF-β、OPG表达量较A组及B组多.结论干细胞介入治疗股骨头缺血性坏死有一定疗效,能改善坏死股骨头血供、促进骨坏死修复.实验结果可为早期治疗股骨头坏死、预防病灶塌陷提供理论依据.
목적 관찰간세포개입치료고골두결혈성배사적료효.방법 필격견18지,수궤분위삼조:A조(대조조)、B조(우귀음조)、C조(간세포개입조)각6지.통과액담냉동법,건립필격견고골두결혈성배사모형.분리、배양병표기골수문질간세포.조모후3주,B조급여우귀음관위,A조여C조이증류수관위;C조동맥관주표기후적골수간질간세포(5×106～1×107/ml)1 m1,A조급B조관주생리염수.치료후4주급8주관찰고골두대체、영상학、조직병이학개변,급고골두공혈혈관적수량、직경.채용면역조화염색관찰VEGF、Brdu적양성표체,통과RT-PCR측정VEGF、TGF-β、OPG、RANKL적표체.결과 C조공혈혈관분지증조、증다,조새혈관재통,혈관수량증다、직경증대.B조、C조관절연골、골소량적결구화형태교A조개선;면역조화검측C조VEGF양성표체솔교A조급B조고;RT-PCR검측C조VEGF、TGF-β、OPG표체량교A조급B조다.결론 간세포개입치료고골두결혈성배사유일정료효,능개선배사고골두혈공、촉진골배사수복.실험결과가위조기치료고골두배사、예방병조탑함제공이론의거.
Objective To evaluate the effect of the method with MSCs interventional therapy on os-teonecrosis of the femoral head (ONFH). Methods Eighteen adult Beagle canines were randomized divided into three groups: A (control group), B (Yougui drink group), C (artery perfusion with MSCs group). The model of ONFH was set up through the method of liquid Nitrogen cryopreservation. At the mean time, MSCs were derived, cultured, proliferated and marked. Three weeks after modeling, Yougui drink (100 ml per ani-mal) was given to the canines in group B by the intragastric administration, distilled water was given to those in group A and group C, respectively. Those in group C were administrated 1 ml MSCs per animal with artery perfusion (5×106-1×107/ml). Those in group A and B were treated with normal saline. At 4 and 8 weeks after continuous treatment, changes of the femoral head were evaluated by methods of gross observation, MRI, his-tologic observation, immunohistochemistry and RT-PCR. Results The quantity and diameter of vessel in group C were significantly better than before therapy. Histologic observation showed that articular cartilage, morphology and structure of trabecular bone in group B and C were better than group A. Immunohistochemistry showed that the VEGF positive staining in group C was significantly higher than in group A and B. The expression of VEGF, TGF-β, OPG genes in group C was higher than in group A and B by RT-PCR. Conclu-sion The method of MSCs interventional therapy, which were performed with artery perfusion seemed to have some therapeutic effect on ONFH. It can improve the blood supply and accelerate repair of femoral head. The final results of this animal study can offer the effective clinical theoretical foundation for the heal-ing of the early stage of ONFH and the prevention of collapse of femoral head.