样品采集和储存所用耗材对微量元素定量分析的影响
양품채집화저존소용모재대미량원소정량분석적영향
Impact of specimen collection and storage consumable products on trace element quantitative analysis
  • 万方数据
    中华预防医学杂志 중화예방의학잡지
    CHINESE JOURNAL OF
    2012年 7期 640-643 ,共4页

    宋艳双%顾永恩%巴特%翟敏%濮吉%沈臻霖%唐仕川%贾光

    송염쌍%고영은%파특%적민%복길%침진림%당사천%가광

    质量控制%痕量元素%样品采集 質量控製%痕量元素%樣品採集
    질량공제%흔량원소%양품채집
    Quality control%Trace elements%Specimen collections
    目的 探讨样品采集、储存所用耗材对微量元素定量分析的影响.方法 市售A、B、C、D4种品牌尿液采集和储存耗材(每种2个样品),经体积分数为1％的HNO3浸泡2～4h；O、P、Q品牌采血装置(每种3个样品)采集超纯水,模拟采血过程；X、Y、Z品牌粉尘采样滤膜(每种2个样品),以浓硝酸冷消化12 h后进行微波消解.对上述采集、储存过程中所得溶液以电感耦合等离子体质谱仪测定镉、钴、铬、铜、铁、锰、钼、镍、铅、硒、锡、钛、钒、锌等14种元素的浓度.结果 尿液采集和储存耗材的元素本底值以均数表示,B品牌5ml冻存管所检测14种元素本底值均较低,其含量范围为0.001～0.350 ng,/ml.A品牌50ml离心管的铜元素、C品牌5ml冻存管的铬元素、D品牌1.5ml离心管的锌元素本底值较高,分别为1.900、1.095和1.368 ng/ml.血液采集装置元素本底值以(x)±s表示,0、P、Q品牌铬元素的本底值分别为(0.120±0.017)、(0.337±0.093)和(0.360±0.035) ng/ml,铜元素的本底值分别为(0.050±0.001)、(0.017±0.012)和(0.103±0.015) ng/ml,铅元素的本底值分别为(0.057±0.072)、(0.183±0.118)和(0.347±0.006) ng/ml,钛元素的本底值分别为(7.883±0.145)、(8.863±0.190)和(8.613±0.274) ng/ml,锌元素的本底值分别为(2.240±0.573)、(42.140±22.756)和(8.850±3.670) ng/ml.3种品牌采血装置铬、铜、铅、钛、锌元素的本底值差异均有统计学意义(P值均＜0.05).空气采样滤膜元素本底值以均数表示,X品牌滤膜铬、镍元素的本底值较低,分别为17.000、15.400 ng/张,其他元素的本底值较高,镉、钴、铜、铁、锰、钼、铅、硒、锡、钛、钒、锌本底值分别为0.250、0.550、48.500、690.000、25.500、0.900、6.500、10.550、7.950、10.500、0.850、370.000 ng/张.Z品牌铬和镍元素本底值较高,分别为171.000和29.850 ng/张.结论 市售不同品牌样品采集、储存耗材的微量元素本底值有差异,差异较大的元素包括铬、锰、镍、铅、锡和锌.
    목적 탐토양품채집、저존소용모재대미량원소정량분석적영향.방법 시수A、B、C、D4충품패뇨액채집화저존모재(매충2개양품),경체적분수위1％적HNO3침포2～4h；O、P、Q품패채혈장치(매충3개양품)채집초순수,모의채혈과정；X、Y、Z품패분진채양려막(매충2개양품),이농초산랭소화12 h후진행미파소해.대상술채집、저존과정중소득용액이전감우합등리자체질보의측정력、고、락、동、철、맹、목、얼、연、서、석、태、범、자등14충원소적농도.결과 뇨액채집화저존모재적원소본저치이균수표시,B품패5ml동존관소검측14충원소본저치균교저,기함량범위위0.001～0.350 ng,/ml.A품패50ml리심관적동원소、C품패5ml동존관적락원소、D품패1.5ml리심관적자원소본저치교고,분별위1.900、1.095화1.368 ng/ml.혈액채집장치원소본저치이(x)±s표시,0、P、Q품패락원소적본저치분별위(0.120±0.017)、(0.337±0.093)화(0.360±0.035) ng/ml,동원소적본저치분별위(0.050±0.001)、(0.017±0.012)화(0.103±0.015) ng/ml,연원소적본저치분별위(0.057±0.072)、(0.183±0.118)화(0.347±0.006) ng/ml,태원소적본저치분별위(7.883±0.145)、(8.863±0.190)화(8.613±0.274) ng/ml,자원소적본저치분별위(2.240±0.573)、(42.140±22.756)화(8.850±3.670) ng/ml.3충품패채혈장치락、동、연、태、자원소적본저치차이균유통계학의의(P치균＜0.05).공기채양려막원소본저치이균수표시,X품패려막락、얼원소적본저치교저,분별위17.000、15.400 ng/장,기타원소적본저치교고,력、고、동、철、맹、목、연、서、석、태、범、자본저치분별위0.250、0.550、48.500、690.000、25.500、0.900、6.500、10.550、7.950、10.500、0.850、370.000 ng/장.Z품패락화얼원소본저치교고,분별위171.000화29.850 ng/장.결론 시수불동품패양품채집、저존모재적미량원소본저치유차이,차이교대적원소포괄락、맹、얼、연、석화자.
    Objective This study aimed to explore the impact of specimen collection and storage consumable products on trace element quantitative analysis.Methods Devices and consumable products of different brands used in specimen collection or storage were selected and treated separately as below:urine collection and storage tubes ( Brand A,B,C and D,2 samples for each brand) were treated with 1％ of HNO3 volume fraction for 2 - 4 h ;blood taking device ( Brand O,P and Q,3 samples for each brand) were used for ultra-pure water samples collecting as simulation of blood sampling;dust sampling filters ( Brand X,Y and Z,2 samples for each brand) were cold digested by nitric acid for 12 h,followed by microwave digestion.Then cadmium,cobalt,chromium,copper,iron,manganese,molybdenum,nickel,lead,selenium,stannum,titanium,vanadium and zinc concentrations in the solutions obtained during the course of collect or storage were quantified by inductively coupled plasma mass spectrometer.Results For the urine collection and storage consumable products,background values of elements were discribed as mean of parellel samples.The consentration of 14 quantified elements were relatively low for 5 ml cryogenic vials ( brand B) with background values range of 0.001 - 0.350 ng/ml.The background values of copper of 50 ml centrifuge tubes( brand A),chromium of 5 ml cryogenic vials( brand C) and zinc of 1.5 ml centrifuge tubes (brand D) were relatively high,which were 1.900,1.095 and 1.368 ng/ml,respectively.Background values of elements in blood sampling devices were discribed as (x) ± s.Background values of chromium for brand O,P and Q were(0.120 ±0.017),(0.337 ±0.093) and (0.360 ±0.035) ng/ml; for copper were (0.050 ±0.001),(0.017 ±0.012) and (0.103 ±0.015) ng/ml;for lead were (0.057 ±0.072),(0.183 ±0.118)and (0.347 ± 0.006) ng/ml;for titanium were (7.883 ±0.145),(8.863 ±0.190) and (8.613 ±0.274) ng/ml ;zinc were ( 2.240 ± 0.573 ),(42.140 ± 22.756 ) and ( 8.850 ± 3.670 ) ng/ml.There were statistically differences of background values for chromium,copper,lead,titanium and zinc among the above three brands of blood sampling devices( all P values ＜0.05).For air sampling filters,background values of elements were discribed as mean of parellel samples.Background values of chromium and nickel of sampling filters (brand X) were lowest,which were 17.000 and 15.400 ng per piece,respectively;while background values for other elements were relatively high,the quantification of cadmium,cobalt,copper,iron,manganese,molybdenum,lead,selenium,stannum,titanium,vanadium and zinc were 0.250,0.550,48.500,690.000,25.500,0.900,6.500,10.550,7.950,10.500,0.850,370.000 ng per piece,respectively.Background values of chromium and nickel of sampling filters ( brand Z ) were highest,which were 171.000 and 29.850 ng per piece.Conclusion Background values of trace elements varied among products of different brands,and the most noticable differences were found in chromium,manganese,nickel,lead,stannum and zinc.
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