CAJ | 학술논문

目的探讨膳食补充营养素可能对持续高+Gz暴露后小鼠脑功能的影响,用迷宫实验评价脑功能状况.方法将小鼠分为对照组(A组), +Gz暴露但不补充营养素组(B组)及+Gz暴露并且补充营养素组(C组).每组12只小鼠.A组不作+Gz暴露,B组和C组+10 Gz暴露8 min.B组在+Gz暴露前3 h以0.3 ml蒸馏水灌胃.C组在+Gz暴露前1 d饮用强化Vit B6的水,+Gz暴露前3 h以0.3 ml混合氨基酸液灌胃.记录3组动物的迷宫实验成绩.迷宫实验后立即摘眼球取血,测定氨基酸;断头取脑组织,制成组织匀浆,测定单胺类递质.结果 +Gz暴露后, 与对照组相比B组迷宫实验的完成时间显著延长,错误次数增加,C组迷宫实验成绩有改善的趋势,即完成时间缩短,错误次数减少.与B组相比,C组小鼠脑组织中5-羟色胺(5-HT)与多巴胺(DA)的比值显著下降.B组和C组脑内γ谷氨酰基转移酶(GGT)活性显著增加.结论持续高+Gz暴露显著降低小鼠迷宫实验成绩.膳食补充某些氨基酸和Vit B6有改善迷宫成绩的趋势,可能与小鼠脑组织中5-HT与DA的比值显著下降有关.
목적탐토선식보충영양소가능대지속고+Gz폭로후소서뇌공능적영향,용미궁실험평개뇌공능상황.방법장소서분위대조조(A조), +Gz폭로단불보충영양소조(B조)급+Gz폭로병차보충영양소조(C조).매조12지소서.A조불작+Gz폭로,B조화C조+10 Gz폭로8 min.B조재+Gz폭로전3 h이0.3 ml증류수관위.C조재+Gz폭로전1 d음용강화Vit B6적수,+Gz폭로전3 h이0.3 ml혼합안기산액관위.기록3조동물적미궁실험성적.미궁실험후립즉적안구취혈,측정안기산;단두취뇌조직,제성조직균장,측정단알류체질.결과 +Gz폭로후, 여대조조상비B조미궁실험적완성시간현저연장,착오차수증가,C조미궁실험성적유개선적추세,즉완성시간축단,착오차수감소.여B조상비,C조소서뇌조직중5-간색알(5-HT)여다파알(DA)적비치현저하강.B조화C조뇌내γ곡안선기전이매(GGT)활성현저증가.결론지속고+Gz폭로현저강저소서미궁실험성적.선식보충모사안기산화Vit B6유개선미궁성적적추세,가능여소서뇌조직중5-HT여DA적비치현저하강유관.
Objective To explore the possible effects of nutritional supplements on brain function as reflected by Water Maze test performance in mice after +Gz exposure.Method Mice were arranged into control group (group A),+Gz group without nutritional supplementation (group B) and +Gz plus nutritional supplementation group (group C).Each group contains 12 mice.Mice in group A were not exposed to +Gz while mice in both group B and group C were exposed to 8 min +10 Gz.Distilled water was gavaged to group B mice 3 h before +Gz exposure.On the day before +Gz exposure pyridoxol fortified water was given and 3 h before exposure mixed amino acids solution were gavaged to group C mice.Water Maze test was done and scores were recorded in all groups.After the Water Maze test was completed,blood was collected through the eyes for serum amino acid determinations and brain tissue was collected by decollation for monoamine determination and γ-glutamyl transferase (GGT) activity evaluation. Result After +Gz exposure,longer completion time and more mistakes were observed in Water Maze test in group B as compared with group A and a trend of improvement in group C was noticed. The ratio of brain 5-HT to dopamine(DA) was significantly reduced in group C as compared with group B.Gamma glutamyl transferase (GGT) activity in brain tissue in group C and group B increased significantly. Conclusion High sustained +Gz exposure significantly reduces Water Maze test performance in mice (longer completion time and more mistakes).It seems that there is a trend of improvement in Water Maze performance in mice in dietary nutritional supplementation group,which might be due to significant reduction in ratio of brain 5-HT to DA in mice with nutritional supplementation.