中国实验血液学杂志
中國實驗血液學雜誌
중국실험혈액학잡지
JOURNAL OF EXPERIMENTAL HEMATOLOGY
2011年
5期
1171-1175
,共5页
陈芹%林江%钱军%姚冬明%钱炜%李云%柴海彦%杨静%王翠竹%张铭%肖高飞
陳芹%林江%錢軍%姚鼕明%錢煒%李雲%柴海彥%楊靜%王翠竹%張銘%肖高飛
진근%림강%전군%요동명%전위%리운%시해언%양정%왕취죽%장명%초고비
螺旋酶抗原%急性髓系白血病%慢性粒细胞白血病
螺鏇酶抗原%急性髓繫白血病%慢性粒細胞白血病
라선매항원%급성수계백혈병%만성립세포백혈병
HAGE cDNA%acute myeloid leukemia%chronic myeloid leukemia
本研究旨在探讨螺旋酶抗原基因(HAGE)在急性髓系白血病(AML)和慢性髓系白血病(CML)中的表达状况和临床意义.应用实时定量PCR(RQ-PCR)方法检测AML和CML患者骨髓单个核细胞中HAGE cDNA的表达.结果表明:74例AML患者中11例(14.8％)存在HAGE过表达(117.12％ -9842.70％,中位434.96％)；HAGE过表达患者年龄显著高于HAGE阴性者(中位年龄分别为67和45岁,p=0.001).HAGE表达在单核细胞亚型AML( M4和M5,7/20,35.0％)中明显高于其它亚型AML(4/54,7.4％)(p=0.007).28例核型正常的AML患者中8例(28.6％)呈HAGE过表达,而40例核型异常的AML中仅3例(7.5％)存在HAGE表达(p =0.041).9/26例(34.6％) CML患者存在HAGE过表达,加速期和急变期患者中HAGE表达率(4/4,100％)明显高于慢性期患者(5/22,22.7％) (p =0.008).结论:HAGE cDNA表达与AML单核细胞亚型类别相关,且与CML疾病进展相关.
本研究旨在探討螺鏇酶抗原基因(HAGE)在急性髓繫白血病(AML)和慢性髓繫白血病(CML)中的錶達狀況和臨床意義.應用實時定量PCR(RQ-PCR)方法檢測AML和CML患者骨髓單箇覈細胞中HAGE cDNA的錶達.結果錶明:74例AML患者中11例(14.8％)存在HAGE過錶達(117.12％ -9842.70％,中位434.96％)；HAGE過錶達患者年齡顯著高于HAGE陰性者(中位年齡分彆為67和45歲,p=0.001).HAGE錶達在單覈細胞亞型AML( M4和M5,7/20,35.0％)中明顯高于其它亞型AML(4/54,7.4％)(p=0.007).28例覈型正常的AML患者中8例(28.6％)呈HAGE過錶達,而40例覈型異常的AML中僅3例(7.5％)存在HAGE錶達(p =0.041).9/26例(34.6％) CML患者存在HAGE過錶達,加速期和急變期患者中HAGE錶達率(4/4,100％)明顯高于慢性期患者(5/22,22.7％) (p =0.008).結論:HAGE cDNA錶達與AML單覈細胞亞型類彆相關,且與CML疾病進展相關.
본연구지재탐토라선매항원기인(HAGE)재급성수계백혈병(AML)화만성수계백혈병(CML)중적표체상황화림상의의.응용실시정량PCR(RQ-PCR)방법검측AML화CML환자골수단개핵세포중HAGE cDNA적표체.결과표명:74례AML환자중11례(14.8％)존재HAGE과표체(117.12％ -9842.70％,중위434.96％)；HAGE과표체환자년령현저고우HAGE음성자(중위년령분별위67화45세,p=0.001).HAGE표체재단핵세포아형AML( M4화M5,7/20,35.0％)중명현고우기타아형AML(4/54,7.4％)(p=0.007).28례핵형정상적AML환자중8례(28.6％)정HAGE과표체,이40례핵형이상적AML중부3례(7.5％)존재HAGE표체(p =0.041).9/26례(34.6％) CML환자존재HAGE과표체,가속기화급변기환자중HAGE표체솔(4/4,100％)명현고우만성기환자(5/22,22.7％) (p =0.008).결론:HAGE cDNA표체여AML단핵세포아형유별상관,차여CML질병진전상관.
The aim of this study was to investigate the expression status of the helicase antigen (HAGE) transcript and its clinical significance in patients with acute myeloid leukemia (AML) and chronic myeloid leukemia (CML).The expression of HAGE cDNA in bone marrow mononuclear cells from AML and CML patients was detected by using realtime quantitative PCR.The resuts indicated that overexpression of HAGE transcript ( 117.12％ - 9842.70％,median 434.96％ ) was detected in 14.8％ ( 11/74 ) AML patients.AML patients with HAGE cDNA expression were significantly older than thoseHAGE-negative patients (median 67 and 45 years,respectively,p =0.001 ).HAGE cDNA expression was more frequently present among the patients with acute monoblastic leukemia ( M4 and M5,7 of 20,35.0％ ),compared to the patients with acute non-monoblastic leukemia (M1,M2,M3 and M6,4 of 54,7.4％ ) (p =0.007).28.6％ (8/28) cases with normal karyotypes showed HAGE cDNA overexpression,significantly higher than 7.5％ ( 3 of 40) in those with chromosomal abnormalities (p = 0.041 ).Overexpression of HAGE transcript was found in 9 (34.6％) CML cases and more frequently observed at accelerated phase and blast crisis (4/4,100％ ) than that at chronic phase (5/22,22.7％ ) (p ＝0.008 ).It is concluded that HAGE cDNA expression is relevant to specific subtypes of AML and to the progression of CML.
