中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
5期
300-303
,共4页
孙磊%郎振为%王鹏%李坪%赵红心%滕晓英%周新刚%张亮%沈冰%兰孟东
孫磊%郎振為%王鵬%李坪%趙紅心%滕曉英%週新剛%張亮%瀋冰%蘭孟東
손뢰%랑진위%왕붕%리평%조홍심%등효영%주신강%장량%침빙%란맹동
HIV感染%肠黏膜%CD4+T淋巴细胞%T淋巴细胞,调节性%转录因子
HIV感染%腸黏膜%CD4+T淋巴細胞%T淋巴細胞,調節性%轉錄因子
HIV감염%장점막%CD4+T림파세포%T림파세포,조절성%전록인자
HIV infections%Intestinal mucosa%CD4 positive T-lymphocytes%T-lymphocytes,regulatory%Transcription factors
目的 研究HIV感染者肠黏膜组织中CD4+CD25+调节性T淋巴细胞(Treg)及叉头状转录因子3(FoxP3)的表达.方法 应用免疫组织化学法,检测21例HIV感染者及17例非HIV感染者肠黏膜组织中CD4+CD25+Treg的特异性标志物FoxP3的表达以及CD4+T淋巴细胞的原位表达.数据处理应用t检验.结果 HIV感染者肠黏膜组织中CD4+T淋巴细胞阳性标记指数为11.56%±4.44%,显著低于非HIV感染者的43.49%±8.90%(t=-11.86,P<0.01).HIV感染者肠黏膜组织中FoxP3阳性标记指数为0.46%±0.20%,显著低于非HIV感染者的1.18%±0.44%(t=-5.98,P<0.01).结论 HIV感染者肠黏膜组织中CD4+T淋巴细胞出现消减,CD4+CD25+Treg也出现消减,FoxP3表达下降.
目的 研究HIV感染者腸黏膜組織中CD4+CD25+調節性T淋巴細胞(Treg)及扠頭狀轉錄因子3(FoxP3)的錶達.方法 應用免疫組織化學法,檢測21例HIV感染者及17例非HIV感染者腸黏膜組織中CD4+CD25+Treg的特異性標誌物FoxP3的錶達以及CD4+T淋巴細胞的原位錶達.數據處理應用t檢驗.結果 HIV感染者腸黏膜組織中CD4+T淋巴細胞暘性標記指數為11.56%±4.44%,顯著低于非HIV感染者的43.49%±8.90%(t=-11.86,P<0.01).HIV感染者腸黏膜組織中FoxP3暘性標記指數為0.46%±0.20%,顯著低于非HIV感染者的1.18%±0.44%(t=-5.98,P<0.01).結論 HIV感染者腸黏膜組織中CD4+T淋巴細胞齣現消減,CD4+CD25+Treg也齣現消減,FoxP3錶達下降.
목적 연구HIV감염자장점막조직중CD4+CD25+조절성T림파세포(Treg)급차두상전록인자3(FoxP3)적표체.방법 응용면역조직화학법,검측21례HIV감염자급17례비HIV감염자장점막조직중CD4+CD25+Treg적특이성표지물FoxP3적표체이급CD4+T림파세포적원위표체.수거처리응용t검험.결과 HIV감염자장점막조직중CD4+T림파세포양성표기지수위11.56%±4.44%,현저저우비HIV감염자적43.49%±8.90%(t=-11.86,P<0.01).HIV감염자장점막조직중FoxP3양성표기지수위0.46%±0.20%,현저저우비HIV감염자적1.18%±0.44%(t=-5.98,P<0.01).결론 HIV감염자장점막조직중CD4+T림파세포출현소감,CD4+CD25+Treg야출현소감,FoxP3표체하강.
Objective To investigate the changes of CD4+ CD25+ regulatory T lymphocyte (Treg) and expressions of folkhead helix transcription factor 3 (FoxP3) in intestinal mucosa in human immunodeficiency virus (HIV) infected patients. Methods Twenty-one HIV infected patients and 17 control subjects without HIV infection were included in this study. The expression of FoxP3, which was considered as a specific marker of CD4+ CD25 + Treg, was detected in intestinal mucosa specimens from HIV infected patients by immunohistochemistry. Meanwhile, the in situ expression of CD4+ T lymphocyte was also determined by immunohistochemistry. The data were analyzed by t test. Results The positive labeling index of CD4+ T lymphocyte in intestinal mucosa was significantly lower in HIV infected patients compared to the controls (11. 56%±4. 44% vs 43. 49% ±8. 90% ,t=-11. 86,P<0. 01). The positive labeling index of FoxP3 in intestinal mucosa was also significantly lower in HIV infected patients compared to the controls (0.46% ± 0.20% vs 1. 18% ± 0. 44% ,t= - 5. 98,P<0.01). Conclusion The depletion of CD4+ CD25+ Treg is accompanied with the depletion of CD4 + T lymphocyte and the reduction of FoxP3 expression in intestinal mucosa of HIV infected patients.
