中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2008年
4期
255-257
,共3页
何贵金%鞠光%于发强%高沁怡%许书河%高红%姜维国%蒋涛%戴显伟%马凯
何貴金%鞠光%于髮彊%高沁怡%許書河%高紅%薑維國%蔣濤%戴顯偉%馬凱
하귀금%국광%우발강%고심이%허서하%고홍%강유국%장도%대현위%마개
细胞凋亡%103Pd支架%γ射线%bcl-2基因
細胞凋亡%103Pd支架%γ射線%bcl-2基因
세포조망%103Pd지가%γ사선%bcl-2기인
Apoptosis%103Pd radioactive stent%γ-radiation bcl-2 gene
目的 为探讨γ射线对犬bcl-2基因的影响及意义.方法 将103钯(103Pd)放射性支架和普通支架分别植入犬的肝外胆管,免疫组化方法 检测γ射线诱导的犬胆管壁凋亡增殖平滑肌细胞中的bcl-2表达,计数凋亡细胞,计算机图像分析系统检测两组胆管管腔面积.结果 bcl-2表达103Pd支架组较普通支架组为弱,胆管出现增殖平滑肌细胞明显凋亡;肝外胆管无明显狭窄;普通支架组未出现增殖平滑肌细胞明显凋亡,肝外胆管有明显狭窄.结论 bcl-2表达水平与细胞凋亡发生和细胞对辐射敏感性有关;103Pd放射性支架通过降低bcl-2表达,促进胆管增殖平滑肌细胞凋亡,从而抑制肝外胆管狭窄.
目的 為探討γ射線對犬bcl-2基因的影響及意義.方法 將103鈀(103Pd)放射性支架和普通支架分彆植入犬的肝外膽管,免疫組化方法 檢測γ射線誘導的犬膽管壁凋亡增殖平滑肌細胞中的bcl-2錶達,計數凋亡細胞,計算機圖像分析繫統檢測兩組膽管管腔麵積.結果 bcl-2錶達103Pd支架組較普通支架組為弱,膽管齣現增殖平滑肌細胞明顯凋亡;肝外膽管無明顯狹窄;普通支架組未齣現增殖平滑肌細胞明顯凋亡,肝外膽管有明顯狹窄.結論 bcl-2錶達水平與細胞凋亡髮生和細胞對輻射敏感性有關;103Pd放射性支架通過降低bcl-2錶達,促進膽管增殖平滑肌細胞凋亡,從而抑製肝外膽管狹窄.
목적 위탐토γ사선대견bcl-2기인적영향급의의.방법 장103파(103Pd)방사성지가화보통지가분별식입견적간외담관,면역조화방법 검측γ사선유도적견담관벽조망증식평활기세포중적bcl-2표체,계수조망세포,계산궤도상분석계통검측량조담관관강면적.결과 bcl-2표체103Pd지가조교보통지가조위약,담관출현증식평활기세포명현조망;간외담관무명현협착;보통지가조미출현증식평활기세포명현조망,간외담관유명현협착.결론 bcl-2표체수평여세포조망발생화세포대복사민감성유관;103Pd방사성지가통과강저bcl-2표체,촉진담관증식평활기세포조망,종이억제간외담관협착.
Objective To study the expression and significance of bcl-2 gene in the apoptotic muscle cells in γ-radiation-induced muscle cell lines.Methods bcl-2 gene in the control and r-radiation-induced apoptotic muscle cells was analyzed by using immunohistochemical technique.Results The bcl-2 gene transcript more highly expressed in general stent dog bile duct than in 103Pd radioactive stent dog bile duct,but apoptotic muscle cells were more highly in 103Pd radioactive stent dog bile duct than in general stent dog bile duct.Conclusion The low level of expression of bcl-2 gene may aid the effects tO understand the muscle cell sensitivity to γ-radiation apoptosis.103Pd radioactive stent may reduce the expression of bcl-2 gene in dog bile duct and prevent the biliary narrowing after dog bile duct injury by balloon.
