CAJ | 학술논문

目的检测Ⅱ型胶原、基质金属蛋白酶7(MMP-7)在发育性髋关节发育不良(DDH)早期髋臼软骨中表达的改变情况,探讨Ⅱ型胶原、MMP-7与软骨退变的相关性.方法兔左下肢作为实验侧,膝关节伸直位、长腿管形石膏固定;右下肢作为对照侧,5周后经X线证实构建DDH动物模型8只.观察实验组和对照组髋臼软骨形态学改变;免疫组化两步法、免疫印迹方法检测Ⅱ型胶原、MMP-7在髋臼软骨中表达的改变.结果实验组可见有局部软骨细胞簇集,细胞外基质甲苯胺蓝染色有失染现象.免疫组化染色结果显示实验组Ⅱ型胶原和MMP-7阳性染色细胞明显高于对照组.免疫印迹结果显示实验组Ⅱ型胶原和MMP-7蛋白表达量均升高,组间比较差异均有统计学意义(t=2.18,t=2.334,P<0.05).结论 DDH早期髋臼软骨发生退行性变,Ⅱ型胶原、MMP-7高表达,提示Ⅱ型胶原、MMP-7的数量和强度可能与软骨退行性变的程度差异有关.
목적 검측Ⅱ형효원、기질금속단백매7(MMP-7)재발육성관관절발육불량(DDH)조기관구연골중표체적개변정황,탐토Ⅱ형효원、MMP-7여연골퇴변적상관성.방법 토좌하지작위실험측,슬관절신직위、장퇴관형석고고정;우하지작위대조측,5주후경X선증실구건DDH동물모형8지.관찰실험조화대조조관구연골형태학개변;면역조화량보법、면역인적방법검측Ⅱ형효원、MMP-7재관구연골중표체적개변.결과 실험조가견유국부연골세포족집,세포외기질갑분알람염색유실염현상.면역조화염색결과현시실험조Ⅱ형효원화MMP-7양성염색세포명현고우대조조.면역인적결과현시실험조Ⅱ형효원화MMP-7단백표체량균승고,조간비교차이균유통계학의의(t=2.18,t=2.334,P<0.05).결론 DDH조기관구연골발생퇴행성변,Ⅱ형효원、MMP-7고표체,제시Ⅱ형효원、MMP-7적수량화강도가능여연골퇴행성변적정도차이유관.
Objective To detect the changes of expressions of Ⅱ type collagen and matrix metalloproteinases-7 ( MMP-7 ) of acetabular cartilage in early DDH ( developmental dysplasia of hip) and to investigate the relevance between Ⅱ type collagen and MMP-7 and the retrogression mechanism of acetabular cartilage. Methods The animal model of DDH was successfully established in 8 rabbits by applying the method of knee extension in which left lower extremity as experimental group and right one as control group.And the stains of HE and toluidine blue were applied on the samples of acetabular cartilage to observe the changes of chondrocytes and extracellular matrix ( ECM ). The techniques of immunohistocbemical staining and Western blot were employed to respectively qualify and quantitate the expression of Ⅱ type collagen and MMP-7. Results Pathohistological observation indicated the signs of retrogressive changes of acetabular cartilage in experimental group, including a loss of ECM in toluidine blue stain and a cluster of chondrocytes in HE stain. The positive numbers of Ⅱ type collagen and MMP-7 by immunohistochemical staining in experimental group were beth higher than that of control group. The quantitative amounts of Ⅱ type collagen and MMP-7 by Western blot in experimental group were both higher than that of control group. Both significant differences existed between two groups ( P < 0. 05 ). Conclusion The expression of Ⅱ type collagen and MMP-7 is correlated to a retrogression of acetabular cartilage and increases obviously in early DDH. The amount and intensity of Ⅱ type collagen and MMP-7 are probably the rationale of differential retrogression of cartilage.