农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
2期
81-84,128
,共5页
刘立科%刘根齐%侯宁%刘春光%陈建南%张文会%樊颖伦%吴冰洁
劉立科%劉根齊%侯寧%劉春光%陳建南%張文會%樊穎倫%吳冰潔
류립과%류근제%후저%류춘광%진건남%장문회%번영륜%오빙길
HSP70%Osg6B promoter%Gene engineering%Male sterility
[Objective] In order to study the relation between the HSP70 gene and male sterility of plant further. [Methods] Anther specific expression promoter Osg6B of rice was coloned by PCR then connected with HStrlO antisense fragment to construct HSP70 antisense expression vector. The expression vector was identified by PCR experiment and enzyme digestion. [Result]The sequence of coloned Osg6B promoter had 97% homology to the published se-quence, and the cis-regulatory element in promoter area was integrated. HSIrlO antisense expression vector driven by the promoter Osg6B was confired by colony PCR and enzyme digestion. [Conclusion]The construction of expressio~a vector would lay solid foundation for utilization of genetic,engineering male sterility of plant.
