CAJ | 학술논문

利用塑料半薄切片对水稻同源四倍体亚种间杂种F1及其对应的二倍体杂种F1小孢子母细胞减数分裂过程的细胞学变化进行观察研究.结果表明,同源四倍体水稻亚种间杂种造孢细胞期和小孢子母细胞期已经表现出较高频率的异常;减数分裂过程中,小孢子母细胞出现异常更加复杂,主要包括小孢子母细胞液泡化和退化两大类,这些异常是导致杂种花粉败育和花粉低育性的重要原因之一;此外,绒毡层异常也是导致杂种的花粉育性降低的因素.二倍体杂种小孢子母细胞在减数分裂过程的异常类型与水稻同源四倍体亚种间杂种基本相似,其绒毡层异常频率较低,对其花粉低育性影响不大.
이용소료반박절편대수도동원사배체아충간잡충F1급기대응적이배체잡충F1소포자모세포감수분렬과정적세포학변화진행관찰연구.결과표명,동원사배체수도아충간잡충조포세포기화소포자모세포기이경표현출교고빈솔적이상;감수분렬과정중,소포자모세포출현이상경가복잡,주요포괄소포자모세포액포화화퇴화량대류,저사이상시도치잡충화분패육화화분저육성적중요원인지일;차외,융전층이상야시도치잡충적화분육성강저적인소.이배체잡충소포자모세포재감수분렬과정적이상류형여수도동원사배체아충간잡충기본상사,기융전층이상빈솔교저,대기화분저육성영향불대.
Cytological observation on microsporogenesis of inter-subspecific hybrids of autotetraploid and corresponding diploid rice was conducted by using techniques of plastic semi-thin.The results indicated that inter-subspecific hybrid of autotetraploid appeared high ratio of abnormalities in sporogenous cell stage and microsporocyte stage.In the process of meiosis,abnormalities of microsporocyte became more complex,mainly including microsporocyte vacuolization and degeneration which was one of the key factors inducing pollen abortion and low pollen fertility of hybrid rice.In addition,abnormality of tapetum was also the main reason for low pollen fertility of hybrid.Types of abnormalities in microsporogenesis of inter-subspecific hybrid of diploid were similar to those of autotetraploid hybrid.Abnormalities of tapetum in microsporogenesis of inter-subspecific hybrid of diploid rice were fewer than that of tetraploid.