CAJ | 학술논문

采用RAPD技术对分离自河南省各地的43株小麦黑胚病优势病原菌(Altemaria spp.)菌株进行了遗传多样性分析.17个随机引物共扩增出151条清晰的DNA条带,所扩增出的DNA条带均为多态带,说明河南省小麦黑胚病菌存在着丰富的遗传多样性.利用NTSYS软件进行了病原菌的聚类分析,结果表明,河南省小麦黑胚病菌主要有2个种.即Alternaria alternata和A.tenuissima,种间的遗传相似系数的变化幅度为0.62～0.92.来自同一个地区的小麦黑胚病菌菌株基本上聚在了一起,表现出很近的亲缘关系,来自不同地区之间的菌株也可以交叉聚类.病原菌遗传多样性分析进一步验证了形态学的鉴定结果.
채용RAPD기술대분리자하남성각지적43주소맥흑배병우세병원균(Altemaria spp.)균주진행료유전다양성분석.17개수궤인물공확증출151조청석적DNA조대,소확증출적DNA조대균위다태대,설명하남성소맥흑배병균존재착봉부적유전다양성.이용NTSYS연건진행료병원균적취류분석,결과표명,하남성소맥흑배병균주요유2개충.즉Alternaria alternata화A.tenuissima,충간적유전상사계수적변화폭도위0.62～0.92.래자동일개지구적소맥흑배병균균주기본상취재료일기,표현출흔근적친연관계,래자불동지구지간적균주야가이교차취류.병원균유전다양성분석진일보험증료형태학적감정결과.
The genetic diversity of 43 Alternaria spp. isolates from the wheat black point of six representative areas in Henan Province was analyzed by RAPD. 151 RAPD markers were obtained, which were amplified by 17 random primers, with 100% polymorphisms. It showed that rich genetic diversity of the pathogens of wheat black point was existed in Henan Province. The results of NTSYS cluster analysis showed that Alternaria alter-nata and A. tenuissima were the main pathogens of wheat black point, and the similarity coefficients were ranged from 0.62 to 0.92. Most of the isolates from the same area were clustered together and showed close genetic relationships. Some isolates from different areas were also clustered together with similarity. The results of genetic diversity analysis to the isolates had basically proved the morphological identification.