复旦学报（医学版）
複旦學報（醫學版）
복단학보（의학판）
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2010年
1期
106-109
,共4页
刘岩%张瑞帆%翁欢%李秋华
劉巖%張瑞帆%翁歡%李鞦華
류암%장서범%옹환%리추화
光化学法%视网膜动脉阻塞%动物模型
光化學法%視網膜動脈阻塞%動物模型
광화학법%시망막동맥조새%동물모형
photochemistry%retinal artery occlusion%animal model
目的 探讨应用倍频532 nm激光通过光化学法建立视网膜动脉阻塞(retinal artery occlusion,RAO)动物模型的可行性.方法 健康成年新西兰白兔10只,自耳缘静脉注入5%孟加拉红,应用倍频532 nm激光通过光化学法制备RAO模型,并于1 h、24 h、3 d行直接眼底镜、眼底照相和眼底荧光血管造影(fundus fluorescein angiography,FFA)检查;3 d后将实验兔以空气栓塞法处死,摘除眼球行组织学检查.结果 9只实验眼经直接眼底镜观察和FFA检查证实建立RAO, 眼球组织切片光镜下检查可见视网膜动脉内血栓,血管壁基本完整.结论 应用用倍频532 nm激光通过光化学法能成功建立兔RAO模型,且组织学检查证实动脉血栓形成同时并无明显的血管壁损伤;此方法成功率高、安全性好、重复性好,可作为探索RAO治疗方法的试验动物模型.
目的 探討應用倍頻532 nm激光通過光化學法建立視網膜動脈阻塞(retinal artery occlusion,RAO)動物模型的可行性.方法 健康成年新西蘭白兔10隻,自耳緣靜脈註入5%孟加拉紅,應用倍頻532 nm激光通過光化學法製備RAO模型,併于1 h、24 h、3 d行直接眼底鏡、眼底照相和眼底熒光血管造影(fundus fluorescein angiography,FFA)檢查;3 d後將實驗兔以空氣栓塞法處死,摘除眼毬行組織學檢查.結果 9隻實驗眼經直接眼底鏡觀察和FFA檢查證實建立RAO, 眼毬組織切片光鏡下檢查可見視網膜動脈內血栓,血管壁基本完整.結論 應用用倍頻532 nm激光通過光化學法能成功建立兔RAO模型,且組織學檢查證實動脈血栓形成同時併無明顯的血管壁損傷;此方法成功率高、安全性好、重複性好,可作為探索RAO治療方法的試驗動物模型.
목적 탐토응용배빈532 nm격광통과광화학법건립시망막동맥조새(retinal artery occlusion,RAO)동물모형적가행성.방법 건강성년신서란백토10지,자이연정맥주입5%맹가랍홍,응용배빈532 nm격광통과광화학법제비RAO모형,병우1 h、24 h、3 d행직접안저경、안저조상화안저형광혈관조영(fundus fluorescein angiography,FFA)검사;3 d후장실험토이공기전새법처사,적제안구행조직학검사.결과 9지실험안경직접안저경관찰화FFA검사증실건립RAO, 안구조직절편광경하검사가견시망막동맥내혈전,혈관벽기본완정.결론 응용용배빈532 nm격광통과광화학법능성공건립토RAO모형,차조직학검사증실동맥혈전형성동시병무명현적혈관벽손상;차방법성공솔고、안전성호、중복성호,가작위탐색RAO치료방법적시험동물모형.
Objective To establish an experimental retinal artery occlusion (RAO) model by means of photochemistry therapy with 532 nm frequency-doubled laser, and to evaluate the feasibility of this method. Methods Ten eyes from ten healthy adult New Zealand rabbits were intravenously injected 5% rose bengal solution, then their monocular retinal artery was irradiated by 532 nm frequency-doubled laser. Both eyes of these rabbits were examined by direct ophthalmoscope and fundus photographic camera on the 1st hour, 24th hour and 3rd day. Meanwhile, the experimental eyes were examined by fundus fluorescein angiography (FFA). These rabbits were killed on the 3rd day, experimental eyes were enucleated, the retina was observed under light microscope. Results There were 9 eyes approved having RAO by direct ophthalmoscope and FFA. Thrombi in retinal arterials could be found by light microscope in all these 9 experimental eyes, and the walls of blood vessel were intact without any obvious necrosis or attenuation. Conclusions RAO animal model can be induced by photochemistry therapy with 532 nm frequency-doubled laser. There are thrombi in the retinal artery without trauma of the artery wall. Photochemistry therapy is an efficient, secure and reproducible method to induce animal model of RAO.
