CAJ | 학술논문

[目的]探讨人类成熟卵丘细胞在未成熟卵母细胞体外成熟培养中的作用,并建立一种简易的实施技术.[方法]在控制性促排卵周期有未成熟卵母细胞时,将同周期成熟卵丘复合体切出部分卵丘细胞,用1 mL注射器抽打分散细胞,贴壁培养.113个治疗周期中,298枚生发泡期卵母细胞经3种不同培养液(A、B、C)体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中):第1组28个周期中73枚(A液):基础培养液+卵泡液;第2组40个周期中115枚(B液):A液+分散贴壁的卵丘细胞;第3组45个周期中110枚(C液):A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子.观察其成熟率、受精率及可用胚胎获得率等.[结果]24 h成熟率:组间比较有显著性差异(A:45.2%,B:61.7%,C:78.2%.P＜0.05);25～48 h无显著意义.成熟卵的正常受精率在59%～67%之间,组间比较无显著差异;与第1组(54.5%,11.0%)相比,第2组(83.3%,25.2%)、第3组(90.7%,37.3%)的卵裂率和挽救率均有显著性差异(P＜0.05),可用胚胎获得率组间比较依次呈现上升趋势(66.7%,82.9%,83.7%).[结论]来自控制性促排卵周期的成熟卵丘细胞经简易吹打分散后贴壁培养,可能能协同卵泡液中或外加的生长因子,促进未成熟卵母细胞的体外成熟,而本研究技术简易有效,可用于挽救促排卵周期的未成熟卵.
[목적]탐토인류성숙란구세포재미성숙란모세포체외성숙배양중적작용,병건립일충간역적실시기술.[방법]재공제성촉배란주기유미성숙란모세포시,장동주기성숙란구복합체절출부분란구세포,용1 mL주사기추타분산세포,첩벽배양.113개치료주기중,298매생발포기란모세포경3충불동배양액(A、B、C)체외성숙배양(동일병인적생발포기란피수궤분도모동일조중):제1조28개주기중73매(A액):기출배양액+란포액;제2조40개주기중115매(B액):A액+분산첩벽적란구세포;제3조45개주기중110매(C액):A액+분산첩벽적란구세포+촉란포생성격소+표피생장인자.관찰기성숙솔、수정솔급가용배태획득솔등.[결과]24 h성숙솔:조간비교유현저성차이(A:45.2%,B:61.7%,C:78.2%.P＜0.05);25～48 h무현저의의.성숙란적정상수정솔재59%～67%지간,조간비교무현저차이;여제1조(54.5%,11.0%)상비,제2조(83.3%,25.2%)、제3조(90.7%,37.3%)적란렬솔화만구솔균유현저성차이(P＜0.05),가용배태획득솔조간비교의차정현상승추세(66.7%,82.9%,83.7%).[결론]래자공제성촉배란주기적성숙란구세포경간역취타분산후첩벽배양,가능능협동란포액중혹외가적생장인자,촉진미성숙란모세포적체외성숙,이본연구기술간역유효,가용우만구촉배란주기적미성숙란.
[Objective]To evaluate the role of mature cumulus cells from oocyte-cumulus complex(OCC)in in-vitro maturation(IVM)and establish a new culture technique which is convenient to carry out.[Methods]The cumulus cells of OCC were cut off and dispersed by 1 mL syringe.The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish.The immature oocytes were experienced IVM procedures in different culture media.They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly).Group 1(solution A):basic culture medium+human follicular fluid(hFF);Group 2(solution B):solution A+ cumulus cells(OCC);Group 3(solution C):solution A+ OCC+ follicle stimulating hormone(FSH)+ epidermal growth factor (EGF).Then,the maturation rate,fertilization rate and formation rate of available embryo were observed.[Results]In 113 treatment cycles,298 immature oocytes were performed IVM with solution A,B,and C.The difference for 24 hour maturation rates among 3 groups wag statistically significant(A:45.2%,B:61.7%,C:78.2%,P＜0.05).There was no statistical difference for 25～48 hour maturation rates and normal fertilization rates of mature oocytes.The differences of cleavage rates and rescued embryo rates between group 1 and 2,group 1 and 3 were statistically significant(P＜0.05).The formation rates of available embryo showed an increasing trend from group 1,2,to 3.[Conclusion]After being dispersed by simply beat upon with syringe and adherent culture,the mature cumulus eells from mature OCCs in COH cycles,together with growth factors in the follicular fluid or extraneously supplemented,could promote the IVM of immature oocyte.