CAJ | 학술논문

目的:探讨艾灸治疗糖尿病周围神经病变(Diabetic Peripheral Neuropathy,DPN)的周围神经保护机制.方法:以链脲佐菌素(Streptozotocin,STZ)腹腔注射诱导形成DPN模型,艾灸大鼠胰俞、足三里穴,每日1次,每穴15 min,连续治疗56d.通过血糖、尿糖、体重、饮食量等的监测,结合神经电生理方法检测神经传导速度,评价艾灸对DPN的治疗效应;HE染色、光镜观察坐骨神经组织结构变化;酶联免疫吸附法检测坐骨神经NGF含量.结果:治疗后,艾灸组大鼠血糖水平明显低于模型组(P＜0.01);艾灸组感觉神经传导速度明显快于模型组(P＜0.01);艾灸组病理形态改变较模型组明显;艾灸组NGF含量也明显高于模型组(P＜0.01).结论:艾灸能有效改善DPN模型大鼠周围神经病变症状、体征.艾灸改善DPN模型大鼠周围神经症状的效应可能与提高NGF含量,促进周围神经保护有关.
목적:탐토애구치료당뇨병주위신경병변(Diabetic Peripheral Neuropathy,DPN)적주위신경보호궤제.방법:이련뇨좌균소(Streptozotocin,STZ)복강주사유도형성DPN모형,애구대서이유、족삼리혈,매일1차,매혈15 min,련속치료56d.통과혈당、뇨당、체중、음식량등적감측,결합신경전생리방법검측신경전도속도,평개애구대DPN적치료효응;HE염색、광경관찰좌골신경조직결구변화;매련면역흡부법검측좌골신경NGF함량.결과:치료후,애구조대서혈당수평명현저우모형조(P＜0.01);애구조감각신경전도속도명현쾌우모형조(P＜0.01);애구조병리형태개변교모형조명현;애구조NGF함량야명현고우모형조(P＜0.01).결론:애구능유효개선DPN모형대서주위신경병변증상、체정.애구개선DPN모형대서주위신경증상적효응가능여제고NGF함량,촉진주위신경보호유관.
Objective:To study the mechanism of moxibustion therapy on diabetic peripheral neuropathy for the peripheral neuroprotection.Methods:The DPN model was induced by intraperitoneal injection with streptozotocin (STZ).The rats were given moxibustion at the acupoint Yishu (Extra) and the acupoint Zusanli (ST 36).The treatment was carried out once a day and 15 minutes per acupoint,lasting for 56 d in total.The clinical effect of moxibustion was evaluated by detecting blood sugar,urine sugar,body weight and dietary intakes,as well as nerve conduction velocity with neuroelectrophysiological method.The structure variation of sciatic nerve was observed by HE staining and light microscopy,and the level of NGF in the sciatic nerve Was determined by ELISA.Results:Compared with the model group,the plasma glucose was significantly lower in the moxibustion group (P＜0.01),with significantly faster nerve conduction velocity (P＜0.01),more notably changes in pathological appearance (P＜0.01) and higher level of nerve growth factor (NGF)(P＜0.01).Conclusion:Moxibustion could improve the symptom and signs of peripheral neuropathy in rat models with DPN,which may relate to the increased NGF and enhanced peripheral nerve protection.