CAJ | 학술논문

目的探讨RNA干扰(RNA interference,RNAi)沉默信号转导与转录激活因子-3(signal transduction and activators 0f transcription,STAT3)对人胰腺癌细胞株SW1990体内生长能力的影响及其机制.方法构建STAT3短发卡RNA(short hairpin RNA,shRNA)表达载体,稳定转染SW1990细胞,Western blot观察STAT3和磷酸化STAT3(phosphorylated STAT3,p-STAT3)蛋白表达的改变.裸鼠皮下移植瘤模型检测胰腺癌细胞体内生长能力的变化.Western blot检测Bcl-xL和cyclin D1蛋白表达的改变.结果 RNAi后SW1990细胞中STAT3和p-STAT3的蛋白表达分别下降90%和92%(P＜0.05);裸鼠皮下移植瘤模型显示RNAi沉默STAT3后,SW1990细胞体内生长能力明显下降(P＜0.05);RT-PCR显示RNAi抑制STAT3后,SW1990细胞中Bcl-xL和cyclin D1的蛋白表达分别降低56%和50%(P＜0.05).结论 STAT3 shRNA表达载体能有效抑制STAT3基因,并通过下调Bcl-xL和cyclin D1表达,抑制胰腺癌细胞体内生长能力.
목적 탐토RNA간우(RNA interference,RNAi)침묵신호전도여전록격활인자-3(signal transduction and activators 0f transcription,STAT3)대인이선암세포주SW1990체내생장능력적영향급기궤제.방법 구건STAT3단발잡RNA(short hairpin RNA,shRNA)표체재체,은정전염SW1990세포,Western blot관찰STAT3화린산화STAT3(phosphorylated STAT3,p-STAT3)단백표체적개변.라서피하이식류모형검측이선암세포체내생장능력적변화.Western blot검측Bcl-xL화cyclin D1단백표체적개변.결과 RNAi후SW1990세포중STAT3화p-STAT3적단백표체분별하강90%화92%(P＜0.05);라서피하이식류모형현시RNAi침묵STAT3후,SW1990세포체내생장능력명현하강(P＜0.05);RT-PCR현시RNAi억제STAT3후,SW1990세포중Bcl-xL화cyclin D1적단백표체분별강저56%화50%(P＜0.05).결론 STAT3 shRNA표체재체능유효억제STAT3기인,병통과하조Bcl-xL화cyclin D1표체,억제이선암세포체내생장능력.
Objective To investigate the effect and mechanism of RNAi-mediated STAT3 gene silence on human pancreatic cancer cells growth in vivo. Methods STAT3 shRNA expression vector was stably transfected to SW1990 cells. STAT3 and p-STAT3 protein was examined using Western blot. The growth ability of SW1990 cells in vivo was determined in a subcutaneous tumor model of nude mice. Western blot was performed to detect the protein expression of Bcl-xL and cyclin D1. Results The protein expression of STAT3 and p-STAT3 decreased by 90% and 92% by stable transfection of STAT3 shRNA expressing vectors(P ＜0. 05). Inhibition of STAT3 with RNAi significantly inhibited the growth ability of SW1990 cells in vivo( P ＜ 0. 05 ). The tumor weight significantly decreased( P ＜ 0. 05 ). Moreover, the relative Bcl-xL and cyclinD1 protein expression in SW1990-RNAi cells reduced by 56% and 50% compared with that of the parental SW1990 cells, respectively (P ＜ 0. 05). Conclusions Inhibition of STAT3 with RNAi significantly inhibits the growth ability of pancreatic cancer cells through down-regulating Bcl-xL and cyclin D1.