CAJ | 학술논문

目的建立肺炎链球菌血清型分型的PCR简便方法 ,初步了解肺炎链球菌血清型/群的分布状况.方法设计合成12种肺炎链球菌血清型/群特异性引物,优化不同血清型/群引物FCR条件,并检测最佳反应浓度、灵敏性以及特异性;初步应用于肺炎链球菌菌株的血清型/群检测.结果引物浓度优化后,12种肺炎链球菌血清型/群特异引物呈现较好的特异性与敏感性;对119株肺炎链球菌菌株进行PCR分型检测,其中113株可分为9个血清型/群(3、5、6A/B、9A/V、14、18、19A、19F、23F),6株未分群.结论初步建立了12种血清型/群肺炎链球菌PCR分型技术,可用于鉴别人群中主要流行的肺炎链球菌血清型/群.
목적 건립폐염련구균혈청형분형적PCR간편방법 ,초보료해폐염련구균혈청형/군적분포상황.방법 설계합성12충폐염련구균혈청형/군특이성인물,우화불동혈청형/군인물FCR조건,병검측최가반응농도、령민성이급특이성;초보응용우폐염련구균균주적혈청형/군검측.결과인물농도우화후,12충폐염련구균혈청형/군특이인물정현교호적특이성여민감성;대119주폐염련구균균주진행PCR분형검측,기중113주가분위9개혈청형/군(3、5、6A/B、9A/V、14、18、19A、19F、23F),6주미분군.결론 초보건립료12충혈청형/군폐염련구균PCR분형기술,가용우감별인군중주요류행적폐염련구균혈청형/군.
Objective To establish a method based on PCR for serotyping of Streptococcus pneumonia isolates. PCR serotyping method was applied for investigating the serotypes of S. pneumonia strains. Methods 12 pairs of primers targeting different serotypes or S. pneumonia were designed and synthesized. After optimizing the PCR amplification reaction,sensitivity and specificity of each pair was performed. We applied the PCR methods for testing the serotypes of the isolated S. pneumonia strains. Results Each pair of primers showed satisfied PCR sensitivity and specificity. Of all 119 S. pneumonia strains tested by PCR serotyping method,113 isolates were identified (3,5,6A/B,9A/V,14,18,19A,19F,23F) with 6 isolates were unable to be serotyped. Conclusion We deveioped a simple,reliabie and economic method for S. pneumonia serotyping which could be used for testing the serotypes of S. pneumonia that had been prevailed among general population.