CAJ | 학술논문

目的观察血管平滑肌细胞(VSMC)表达变化及巨噬细胞浸润在老年人腹主动脉瘤中的病理学特点. 方法对15例老年人腹主动脉瘤与6例正常腹主动脉组织行HE染色、VanGieson法染色和免疫组织化学染色.用免疫组织化学染色检测α-平滑肌肌动蛋白(α-SMA)、组织蛋白酶B及CD68蛋白表达. 结果老年人腹主动脉瘤病变处胶原容积百分比(9.3±1.9)%,较正常主动脉的(5.3±1.8)%增高(P＜0.05).老年人腹主动脉瘤中组织蛋白酶B和CD68的表达增强分别为0.38+0.07和0.51±0.12,α-SMA表达减弱为0.23±0.05,与正常腹主动脉(分别为0.13±0.06和0.01±0.01,0.33±0.05)比较.差异有统计学意义(P＜0.05). 结论 VSMC相关蛋白表达水平改变及巨噬细胞浸润可能参与了老年人腹主动脉瘤血管壁的破坏.
목적 관찰혈관평활기세포(VSMC)표체변화급거서세포침윤재노년인복주동맥류중적병이학특점. 방법 대15례노년인복주동맥류여6례정상복주동맥조직행HE염색、VanGieson법염색화면역조직화학염색.용면역조직화학염색검측α-평활기기동단백(α-SMA)、조직단백매B급CD68단백표체. 결과 노년인복주동맥류병변처효원용적백분비(9.3±1.9)%,교정상주동맥적(5.3±1.8)%증고(P＜0.05).노년인복주동맥류중조직단백매B화CD68적표체증강분별위0.38+0.07화0.51±0.12,α-SMA표체감약위0.23±0.05,여정상복주동맥(분별위0.13±0.06화0.01±0.01,0.33±0.05)비교.차이유통계학의의(P＜0.05). 결론 VSMC상관단백표체수평개변급거서세포침윤가능삼여료노년인복주동맥류혈관벽적파배.
Objective To investigate the pathological characteristics of the expressions of associated proteins in vascular smooth muscle cells and infiltration of macrophages in elderly abdominal aortic aneurysms. Methods HE stained slices, Van Gieson' s stained slices and immunohistochemical staining were applied to detect protein expression in the tissue sections of 15 cases of elderly abdominal aortic aneurysms and 6 cases of normal abdominal aorta. The protein expressions of α-smooth muscle actin, cathepsin B and CD68 were detected by immunohistochemical staining. Results Compared with normal abdominal aorta, the collagen content was higher in elderly abdominal aortic aneurysms [(9.3 ± 1.9) % vs. (5.3±1.8) %, P ＜ 0.05]. The levels ofexpression of cathepsin B and CD68 were higher in elderly abdominal aortic aneurysms than those in normal abdominal aorta , but the level of expression of α-SMA was lower in elderly abdominal aorticaneurysms than that in normal abdominal aorta[(0.38±0.07) vs. (0.135=0.06), (0.51±0.12) vs.(0.01±0.01), (0.23±0.05) vs. (0.335±0.05) ,respectively, all P＜0.05]. Conclusions Changes in the expression of associated proteins in vascular smooth muscle cells and infiltration of macrophages may participate in the vascular walls destruction in elderly abdominal aortic aneurysms.