中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2008年
5期
281-285
,共5页
蔡霞%李成章%曹正国%杜格非%柳莉华
蔡霞%李成章%曹正國%杜格非%柳莉華
채하%리성장%조정국%두격비%류리화
牙周炎%模型,动物%基质金属蛋白酶类%黄芩苷
牙週炎%模型,動物%基質金屬蛋白酶類%黃芩苷
아주염%모형,동물%기질금속단백매류%황금감
Periodontitis%Models,animal%Matrix metalloproteinases%Baicalin
目的 观察黄芩苷对丝线结扎诱导的大鼠牙周组织炎性破坏的保护作用,并探讨这一作用是否与牙龈组织中基质金属蛋白酶(matrix metalloproteinases,MMP)表达水平的改变有关.方法 将SD大鼠按随机数字表法分为3组,每组9只.用丝线结扎方法建立牙周炎模型,建模第7天处死动物.用药组(B200组)每天灌胃给予黄芩苷200 mg/kg;阴性对照组(L组)每天灌胃药物媒介0.5%羧甲基纤维素钠;空白对照组(C组)不予牙周炎诱导.评价牙槽骨吸收和胶原纤维的面积分数.免疫组化方法检测牙龈组织中MMP-1、MMP-2和MMP-9的表达.结果 B200组的骨吸收值[(0.93±0.04)mm]显著低于L组[(1.03±0.07)mm,P=0.009)];B200组的胶原纤维面积分数[(48.13±18.69)%]显著高于L组[(31.08±14.85)%,P=0.047];与L组相比,应用黄芩苷显著下调了MMP-1(P=0.023)及MMP-9(P=0.042)的水平,并降低了MMP-2的表达(P=0.099).结论 黄芩苷能够减少丝线结扎诱导的大鼠牙周炎的组织破坏,这一作用可能与其抑制MMP-1、MMP-2和MMP-9的表达有关.
目的 觀察黃芩苷對絲線結扎誘導的大鼠牙週組織炎性破壞的保護作用,併探討這一作用是否與牙齦組織中基質金屬蛋白酶(matrix metalloproteinases,MMP)錶達水平的改變有關.方法 將SD大鼠按隨機數字錶法分為3組,每組9隻.用絲線結扎方法建立牙週炎模型,建模第7天處死動物.用藥組(B200組)每天灌胃給予黃芩苷200 mg/kg;陰性對照組(L組)每天灌胃藥物媒介0.5%羧甲基纖維素鈉;空白對照組(C組)不予牙週炎誘導.評價牙槽骨吸收和膠原纖維的麵積分數.免疫組化方法檢測牙齦組織中MMP-1、MMP-2和MMP-9的錶達.結果 B200組的骨吸收值[(0.93±0.04)mm]顯著低于L組[(1.03±0.07)mm,P=0.009)];B200組的膠原纖維麵積分數[(48.13±18.69)%]顯著高于L組[(31.08±14.85)%,P=0.047];與L組相比,應用黃芩苷顯著下調瞭MMP-1(P=0.023)及MMP-9(P=0.042)的水平,併降低瞭MMP-2的錶達(P=0.099).結論 黃芩苷能夠減少絲線結扎誘導的大鼠牙週炎的組織破壞,這一作用可能與其抑製MMP-1、MMP-2和MMP-9的錶達有關.
목적 관찰황금감대사선결찰유도적대서아주조직염성파배적보호작용,병탐토저일작용시부여아간조직중기질금속단백매(matrix metalloproteinases,MMP)표체수평적개변유관.방법 장SD대서안수궤수자표법분위3조,매조9지.용사선결찰방법건립아주염모형,건모제7천처사동물.용약조(B200조)매천관위급여황금감200 mg/kg;음성대조조(L조)매천관위약물매개0.5%최갑기섬유소납;공백대조조(C조)불여아주염유도.평개아조골흡수화효원섬유적면적분수.면역조화방법검측아간조직중MMP-1、MMP-2화MMP-9적표체.결과 B200조적골흡수치[(0.93±0.04)mm]현저저우L조[(1.03±0.07)mm,P=0.009)];B200조적효원섬유면적분수[(48.13±18.69)%]현저고우L조[(31.08±14.85)%,P=0.047];여L조상비,응용황금감현저하조료MMP-1(P=0.023)급MMP-9(P=0.042)적수평,병강저료MMP-2적표체(P=0.099).결론 황금감능구감소사선결찰유도적대서아주염적조직파배,저일작용가능여기억제MMP-1、MMP-2화MMP-9적표체유관.
Objective To investigate the effect of baicalin on the experimental periodontitis in rats,as well as the expression of MMP-1,MMP-2,MMP-9.Methods Twenty-seven adult male Sprague-Dawley rats were divided into three groups,with 9 rats in each group.A nylon thread was placed around the lower first molars of rats,which were sacrificed after 7 days.Baicalin(200 mg/kg)was administered to the experimental group by oral gavage,starting one day before the induction of periodontitis.The negative control group received vehicle(0.5% carboxymethylcellulose)alone.The blank control group did not get induction of periodontitis.The alveolar bone loss(ABL)and the area fraction(AA%)occupied by collagen fibers were assessed.MMP-1,MMP-2 and MMP-9 protein expressions in the gingiva were detected by immunohistochemistry.Results Baicalin treatment significantly decreased ABL compared with the negative control group(P=0.009).AA% of collagen fibers was significantly higher in baicalin-treated group than in the negative control group(P=0.047).Baicalin treatment significantly down-regulated the protein expression for MMP-1(P=0.023)and MMP-9(P=0.042)and decreased the expression for MMP-2(P=0.099)compared with the negative control group.Conclusions Baicalin protects against tissue damage in ligature-induced periodontitis in rats,which might be mediated in part by its inhibitory effect on the expression of MMP-1,MMP-2 and MMP-9.
