CAJ | 학술논문

目的筛选和鉴定广州管圆线虫未知基因序列,丰富该虫的基因序列数据,为寻找诊断或药物靶点分子提供实验依据.方法构建广州管圆线虫Ⅳ期幼虫cDNA噬菌体文库,随机挑选单个噬菌斑,提取噬菌体DNA进行PCR,扩增其插入的cDNA片段并对PCR产物进行序列测定;采用生物信息学方法对表达序列标签(expressed sequence tags,EST)序列进行分析.结果获得了51条广州管圆线虫基因的EST序列,并提交至GenBank,这些基因包括半乳糖凝集素10,半乳糖凝集素140,表皮蛋白,CRE-RPS-24蛋白等.序列分析结果提示,表皮蛋白和CRE-RPS-24蛋白可能为具有诊断价值的抗原分子,值得进一步研究.结论获得51条广州管圆线虫基因序列,为进一步研究广州管圆线虫致病机制及候选抗原分子提供了新的序列信息.
목적 사선화감정엄주관원선충미지기인서렬,봉부해충적기인서렬수거,위심조진단혹약물파점분자제공실험의거.방법 구건엄주관원선충Ⅳ기유충cDNA서균체문고,수궤도선단개서균반,제취서균체DNA진행PCR,확증기삽입적cDNA편단병대PCR산물진행서렬측정;채용생물신식학방법대표체서렬표첨(expressed sequence tags,EST)서렬진행분석.결과 획득료51조엄주관원선충기인적EST서렬,병제교지GenBank,저사기인포괄반유당응집소10,반유당응집소140,표피단백,CRE-RPS-24단백등.서렬분석결과제시,표피단백화CRE-RPS-24단백가능위구유진단개치적항원분자,치득진일보연구.결론 획득51조엄주관원선충기인서렬,위진일보연구엄주관원선충치병궤제급후선항원분자제공료신적서렬신식.
Objective To screen and identify unknown gene sequences of Angiostrongylus cantonensis for enriching the genetic database of the parasite and providing experimental evidences of drug targets or diagnostic molecules.Methods A 4th stage larvae cDNA library of A.cantonensis was constructed,then phage clones were randomly selected,fostered and sequenced.Meanwhile,all the expressed sequence tags (EST) sequences we got were analyzed by bioinformatics tools.Results 51 EST sequences ofA.cantonensis were obtained and submitted to the GenBank,including galectin 10,galectin 140,cuticle protein,CRE-RPS-24 protein.Sequence analysis showed that cuticle protein and CRE-RPS-24 protein may be antigens with potential diagnostic value.Conclusion 51 EST sequences obtained will be useful for further study on pathogenesis mechanisms and molecular basis for candidate antigens of A.cantonensis.