CAJ | 학술논문

目的探讨鼠源性神经生长因子(mNGF)对HIBD新生大鼠海马胶质纤维酸性蛋白(GFAP)表达的影响.方法新生7日龄SD大鼠60只,随机分为mNGF干预组(n=20)、HIBD组(n=20)、正常对照组(n=20).mNGF干预组在HIBD模型建立后予以mNGF 20 ng/(g·d)臀部肌肉注射,连用5 d.HIBD组未予处置.正常对照组予0.1 mol/L PBS 10μl/(g·d)臀部肌肉注射,连用5 d.3组大鼠在实验开始后7、14 d各处死10只.采用免疫组织化学技术检测3组大鼠海马组织GFAP表达的动态变化.结果在7 d时,mNGF干预组、HIBD组GFAP在病侧海马的表达均较正常对照组增加,但mNGF干预组GFAP主要表达在病侧海马的DG区,HIBD组GFAP主要表达在病侧海马的CA1区.在14d时,HIBD组GFAP在病侧海马的表达减少;mNGF干预组GFAP在病侧海马的表达进一步增加,且较正常对照组增加.结论 mNGF能增加HIBD大鼠海马GFAP的表达,具有神经修复作用.
목적 탐토서원성신경생장인자(mNGF)대HIBD신생대서해마효질섬유산성단백(GFAP)표체적영향.방법 신생7일령SD대서60지,수궤분위mNGF간예조(n=20)、HIBD조(n=20)、정상대조조(n=20).mNGF간예조재HIBD모형건립후여이mNGF 20 ng/(g·d)둔부기육주사,련용5 d.HIBD조미여처치.정상대조조여0.1 mol/L PBS 10μl/(g·d)둔부기육주사,련용5 d.3조대서재실험개시후7、14 d각처사10지.채용면역조직화학기술검측3조대서해마조직GFAP표체적동태변화.결과 재7 d시,mNGF간예조、HIBD조GFAP재병측해마적표체균교정상대조조증가,단mNGF간예조GFAP주요표체재병측해마적DG구,HIBD조GFAP주요표체재병측해마적CA1구.재14d시,HIBD조GFAP재병측해마적표체감소;mNGF간예조GFAP재병측해마적표체진일보증가,차교정상대조조증가.결론 mNGF능증가HIBD대서해마GFAP적표체,구유신경수복작용.
Objective To investigate the influence of mouse nerve growth factor (mNGF)on glial fibrillary acidic protein (GFAP) expression in hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods Sixty neonatal rats of 7 days old were randomly divided into control group (n=20) ,HIBD group (n=20) and mNGF group (n=20). After HIBD model established, mNGF of 20 ng/(g·d) was given by intramuscular injection in buttocks in mNGF group,continued five days. No intervention was given in HIBD group. 0.1mol/L PBS of 10 μ1/(g·d) was given by intramuscular injection in buttocks for the control group,continued five days. Ten rats were sacrified at 7 d and 14 d after intervention respectively in three groups, The expressions of GFAP in hippocampus were determined with immunohistochemical method. Results At 7 d and 14 d after intervention,the expression levels of GFAP in sick side hippocampous in HIBD group and mNGF group were higher than that in control group. GFAP positive cell mainly distributed in sick side hippocampal DG region in mNGF group, while CAI region in HIBD group. As compared with 7 d, the expression of GFAP in sick side hippocampus in HIBD group decreased at 14 d, while increased in mNGF group. Conclusion mNGF can increase the expression of GFAP in hippocampus of HIBD neonatal rat, and mNGF may have nervous repairing role to HIBD.