CAJ | 학술논문

目的通过研究大鼠急性脊髓损伤(SCI)后血清β-淀粉样前体蛋白(β-APP)、超敏C反应蛋白(hs-CRP)不同时间点含量变化,探讨其在继发性SCI中的作用机制及其时程变化规律. 方法选用健康成年雌性SD大鼠90只,采用改良Allen法制备大鼠脊髓完全损伤(完全损伤组,30只)和不全损伤(不完全损伤组,30只)动物模型,另30只大鼠仅行椎板切除作为假手术组.分别于造模成功后12 h、24 h、3d、7d、14d采集股静脉血,ELISA检测不同程度SCI大鼠伤后血清β-APP和hs-CRP含量变化. 结果 β-APP于急性SCI后24h达到最高水平,hs-CRP第3天达到高峰.完全损伤组各时间点β-APP和hs-CRP水平分别与不全损伤组和假手术组比较差异均有统计学意义(P＜0.05)；不全损伤组与假手术组除14 dβ-APP和hs-CRP水平差异无统计学意义(P＞0.05)外,其余各时间点两组的β-APP、hs-CRP水平比较差异均有统计学意义(P＜0.05). 结论急性SCI大鼠血清β-APP、hs-CRP水平的变化反应了SCI的严重程度,β-APP较hs-CRP的变化敏感,在一定时限内可敏感地反映SCI的严重程度及预后.大鼠血清β-APP、hs-CRP的动态变化提示二者共同参与了急性继发性SCI.
목적 통과연구대서급성척수손상(SCI)후혈청β-정분양전체단백(β-APP)、초민C반응단백(hs-CRP)불동시간점함량변화,탐토기재계발성SCI중적작용궤제급기시정변화규률. 방법 선용건강성년자성SD대서90지,채용개량Allen법제비대서척수완전손상(완전손상조,30지)화불전손상(불완전손상조,30지)동물모형,령30지대서부행추판절제작위가수술조.분별우조모성공후12 h、24 h、3d、7d、14d채집고정맥혈,ELISA검측불동정도SCI대서상후혈청β-APP화hs-CRP함량변화. 결과 β-APP우급성SCI후24h체도최고수평,hs-CRP제3천체도고봉.완전손상조각시간점β-APP화hs-CRP수평분별여불전손상조화가수술조비교차이균유통계학의의(P＜0.05)；불전손상조여가수술조제14 dβ-APP화hs-CRP수평차이무통계학의의(P＞0.05)외,기여각시간점량조적β-APP、hs-CRP수평비교차이균유통계학의의(P＜0.05). 결론 급성SCI대서혈청β-APP、hs-CRP수평적변화반응료SCI적엄중정도,β-APP교hs-CRP적변화민감,재일정시한내가민감지반영SCI적엄중정도급예후.대서혈청β-APP、hs-CRP적동태변화제시이자공동삼여료급성계발성SCI.
Objective To investigate the serum levels of β-amyloid precursor protein (β-APP) and hypersensitive-cross-reactive protein (hs-CRP) after acute spinal cord injury (SCI) in rats at different time points and study their roles in secondary spinal cord injury. Methods Ninety healthy adult female SD rats were randomly assigned into 3 even groups.Improved Allen method was used to create models of complete SCI (group A) and models of incomplete SCI (group B).In group C only laminectomy was performed without SCI.The serum was collected at 12 hours,24 hours,3 days,7 days,and 14 days after operation through femoral vein to detect levels of β-APP and hs-CRP by ELISA. Results The serum level of β-APP reached the peak value 24 hours after acute SCI,and the serum level of hs-CRP reached the peak 3 days after acute SCI.There were significant differences between groups A and C in β-APP and hs-CRP levels at all time points ( P ＜ 0.05).There were also significant differences between groups B and C in β-APP and hs-CRP levels at all time points (P ＜0.05) except at 14 days (P＞ 0.05). Conclusions The serum levels of β-APP and hs-CRP are positively associated with the severity of traumatic SCI.β-APP may be a more sensitive indicator than hs-CRP in a certain time.The dynamic changes of serum β-APP and hs-CRP suggest involvement of the 2 proteins in the secondary spinal cord injury.