中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2010年
9期
851-854
,共4页
张金伟%李宝馨%蒋传路%郑永日%胡韶山%葛云龙%付红%韩桂萍%杨宝峰
張金偉%李寶馨%蔣傳路%鄭永日%鬍韶山%葛雲龍%付紅%韓桂萍%楊寶峰
장금위%리보형%장전로%정영일%호소산%갈운룡%부홍%한계평%양보봉
伽玛刀%恒河猴%三叉神经痛%病理%立体定向放射外科
伽瑪刀%恆河猴%三扠神經痛%病理%立體定嚮放射外科
가마도%항하후%삼차신경통%병리%입체정향방사외과
Gamma knife%Macaque%Trigeminal neuralgia%Pathology%Stereotactic radiosurgery
目的 观察伽玛刀照射猴三叉神经的病理组织学变化,探讨伽玛刀治疗原发性三叉神经痛(TN)的放射生物学效应机制和照射神经长度-剂量的效应关系.方法 选择5只恒河猴,1只为对照,4只行伽玛刀照射,选择靶点为三叉神经根,一侧为单靶点照射,对侧为双靶点照射,给予剂量分别为60 Gy、70 Gy、80 Gy、100 Gy.照射后6个月取病理,行光镜、透射电镜及免疫组织化学检查.结果 在相同剂量照射下,单靶点照射与双靶点照射的神经组织结构损伤程度变化基本一致.60 Gy、70 Gy见少许神经纤维脱髓鞘,轴突变性、断裂或消失.80 Gy神经组织结构损伤程度加重.100 Gy神经组织结构损伤程度更重,可见部分神经坏死.结论 60 Gy、70 Gy对三叉神经的组织结构变化影响小,80 Gy可引起三叉神经部分轴突的变性、消失及脱髓鞘,更高的剂量(100 Gy)可导致部分神经坏死.单靶点照射与双靶点照射神经组织结构损伤程度相近,两者的照射神经长度-剂量效应关系差异无统计学意义.
目的 觀察伽瑪刀照射猴三扠神經的病理組織學變化,探討伽瑪刀治療原髮性三扠神經痛(TN)的放射生物學效應機製和照射神經長度-劑量的效應關繫.方法 選擇5隻恆河猴,1隻為對照,4隻行伽瑪刀照射,選擇靶點為三扠神經根,一側為單靶點照射,對側為雙靶點照射,給予劑量分彆為60 Gy、70 Gy、80 Gy、100 Gy.照射後6箇月取病理,行光鏡、透射電鏡及免疫組織化學檢查.結果 在相同劑量照射下,單靶點照射與雙靶點照射的神經組織結構損傷程度變化基本一緻.60 Gy、70 Gy見少許神經纖維脫髓鞘,軸突變性、斷裂或消失.80 Gy神經組織結構損傷程度加重.100 Gy神經組織結構損傷程度更重,可見部分神經壞死.結論 60 Gy、70 Gy對三扠神經的組織結構變化影響小,80 Gy可引起三扠神經部分軸突的變性、消失及脫髓鞘,更高的劑量(100 Gy)可導緻部分神經壞死.單靶點照射與雙靶點照射神經組織結構損傷程度相近,兩者的照射神經長度-劑量效應關繫差異無統計學意義.
목적 관찰가마도조사후삼차신경적병리조직학변화,탐토가마도치료원발성삼차신경통(TN)적방사생물학효응궤제화조사신경장도-제량적효응관계.방법 선택5지항하후,1지위대조,4지행가마도조사,선택파점위삼차신경근,일측위단파점조사,대측위쌍파점조사,급여제량분별위60 Gy、70 Gy、80 Gy、100 Gy.조사후6개월취병리,행광경、투사전경급면역조직화학검사.결과 재상동제량조사하,단파점조사여쌍파점조사적신경조직결구손상정도변화기본일치.60 Gy、70 Gy견소허신경섬유탈수초,축돌변성、단렬혹소실.80 Gy신경조직결구손상정도가중.100 Gy신경조직결구손상정도경중,가견부분신경배사.결론 60 Gy、70 Gy대삼차신경적조직결구변화영향소,80 Gy가인기삼차신경부분축돌적변성、소실급탈수초,경고적제량(100 Gy)가도치부분신경배사.단파점조사여쌍파점조사신경조직결구손상정도상근,량자적조사신경장도-제량효응관계차이무통계학의의.
Objective The authors sought to observe histopathological changes in the trigeminal nerve(TN)after Gamma knife treatment in rhesus monkeys and to investigate the radiobiological mechanism of Gamma knife treatment for primary trigeminal neuralgia. The nerve length dose effect of irradiation was discussed. Method One of five rhesus monkeys randomly served as control and the other four monkeys were randomly administered a target dose of 60,70,80, and 100 Gy respectively. The size of the collimator was 4 mm and the target point was the TN root. In each experimental monkey, one side was exposed to single - target point irradiation and the contralateral side to two - target point irradiation. After 6 months, the TN root was examined by light microscopy, transmission electron microscopy, and immunohistochemistry.Results At the same dose of radiation, the damage to the nervous tissue by single - target point irradiation was identical to that caused by two - target point irradiation. In the trigeminal nerve tissues of the monkeys irradiated with 60 and 70 Gy,there was scant nerve demyelination and degeneration, fragmentation, or loss of axons. In the trigeminal nerve tissue of the monkey irradiated with 80 Gy, the nervous tissue showed a disordered structure. In the trigeminal nerve tissue of the monkey irradiated with 100 Gy, there were severe derangement in the structure of the nervous tissue and extensive demyelination, fragmentation, and loss of axons. Conclusions The target doses of 60 and 70 Gy have very little impact on the structure of the TN. Irradiation at 80 Gy can cause partial degeneration and loss of axons and demyelination. A 100 Gy dose can cause some necrosis of neurons. Comparing the single - target irradiation with the two - target point irradiation,the extent of damage to the nervous tissue is identical and no difference in the nerve length dose effect was found.