中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
6期
1108-1109
,共2页
李文滨%许晓婷%梁思华%褚忠华%赖东明%彭耀荣%王捷%万云乐
李文濱%許曉婷%樑思華%褚忠華%賴東明%彭耀榮%王捷%萬雲樂
리문빈%허효정%량사화%저충화%뢰동명%팽요영%왕첩%만운악
原发性胆囊癌%组织蛋白酶B%组织蛋白酶D%免疫组织化学
原髮性膽囊癌%組織蛋白酶B%組織蛋白酶D%免疫組織化學
원발성담낭암%조직단백매B%조직단백매D%면역조직화학
Primary gallbladder carcinoma%Cathepsin B%Cathepsin D%Immunohistochemistry
目的 探讨原发性胆囊癌、胆囊腺瘤样息肉和慢性胆囊炎组织中组织蛋白酶B(CatB)及组织蛋白酶D(CatD)的表达和相关性及其与临床病理特征之间的关系.方法 采用免疫组织化学染色方法检测32例胆囊癌组织和20例胆囊良性病变(其中胆囊腺瘤样息肉10例,慢性胆囊炎10例)组织中CatB及CatD的表达.结果 CatB、CatD在胆囊癌组织的阳性表达率分别为93.75% (30/32)、90.63% (29/32),均明显高于胆囊腺瘤样息肉组织(分别为60.00%、60.00%,P<0.05)和慢性胆囊炎组织(分别为60.00%、50.00%,P<0.05).CatB、CatD的表达在患者年龄、性别、标本Nevin分期、病理学分级、病理学类型间的差异均无统计学意义(P>0.05).CatB与CatD的表达呈正相关性(r=0.434,P <0.05).结论 CatB和CatD在胆囊癌组织中表达增强,可作为胆囊癌组织学标志物.胆囊组织中CatB和CatD的高表达与临床病理特征无明显相关.CatD与CatB的表达呈正相关.
目的 探討原髮性膽囊癌、膽囊腺瘤樣息肉和慢性膽囊炎組織中組織蛋白酶B(CatB)及組織蛋白酶D(CatD)的錶達和相關性及其與臨床病理特徵之間的關繫.方法 採用免疫組織化學染色方法檢測32例膽囊癌組織和20例膽囊良性病變(其中膽囊腺瘤樣息肉10例,慢性膽囊炎10例)組織中CatB及CatD的錶達.結果 CatB、CatD在膽囊癌組織的暘性錶達率分彆為93.75% (30/32)、90.63% (29/32),均明顯高于膽囊腺瘤樣息肉組織(分彆為60.00%、60.00%,P<0.05)和慢性膽囊炎組織(分彆為60.00%、50.00%,P<0.05).CatB、CatD的錶達在患者年齡、性彆、標本Nevin分期、病理學分級、病理學類型間的差異均無統計學意義(P>0.05).CatB與CatD的錶達呈正相關性(r=0.434,P <0.05).結論 CatB和CatD在膽囊癌組織中錶達增彊,可作為膽囊癌組織學標誌物.膽囊組織中CatB和CatD的高錶達與臨床病理特徵無明顯相關.CatD與CatB的錶達呈正相關.
목적 탐토원발성담낭암、담낭선류양식육화만성담낭염조직중조직단백매B(CatB)급조직단백매D(CatD)적표체화상관성급기여림상병리특정지간적관계.방법 채용면역조직화학염색방법검측32례담낭암조직화20례담낭량성병변(기중담낭선류양식육10례,만성담낭염10례)조직중CatB급CatD적표체.결과 CatB、CatD재담낭암조직적양성표체솔분별위93.75% (30/32)、90.63% (29/32),균명현고우담낭선류양식육조직(분별위60.00%、60.00%,P<0.05)화만성담낭염조직(분별위60.00%、50.00%,P<0.05).CatB、CatD적표체재환자년령、성별、표본Nevin분기、병이학분급、병이학류형간적차이균무통계학의의(P>0.05).CatB여CatD적표체정정상관성(r=0.434,P <0.05).결론 CatB화CatD재담낭암조직중표체증강,가작위담낭암조직학표지물.담낭조직중CatB화CatD적고표체여림상병리특정무명현상관.CatD여CatB적표체정정상관.
Objective To investigate the expression of cathepsin B (CatB) and cathepsin D (CatD) in primary gallbladder carcinoma,adenomatoid polyp without malignant transformation,and chronic cholecystitis tissues and to assess their mutual correlation ships as well as the relationships between their expressions and clinical pathological features.Methods Detected the expressions of CatB and CatD in gallbladder tissues including 32 specimens of primary gallbladder carcinoma,10 specimens of adenomatoid polyp without malignant transformation and 10 specimens of chronic cholecystitis by immunohistochemical method.The expressions were assessed by semi-quantitative analysis.Results The positive rate of CatB,CatD and CCL20 in primary gallbladder carcinoma was 93.75% (30/32) and 90.63% (29/32) respectively,being significantly higher than those in adenomatoid polyp without malignant transformation (60%and 60%,P <0.05) and chronic cholecystitis groups (60% and 50% P <0.05).The expression levels of CatB and CatD were not significantly related to the clinical features including age and gender,or pathological features including grades of cell differentiation,pathological types and Nevin stages ( P > 0.05 ).CatB and CatD had significantly positive correlations ( r =0.434,P < 0.05 ).Conclusion ( 1 ) CatB and CatD CCL20 might serve as the histological marker of primary gallbladder carcinoma.(2) The expression levels of CatB and CatD in primary gallbladder carcinoma are not related to the clinical or pathological features.(3) There is positive correlation between the expression levels of CatB and CatD in primary gallbladder carcinoma.