中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
6期
391-394
,共4页
段化伟%冷曙光%潘祖飞%牛勇%宾萍%戴宇飞%郑玉新
段化偉%冷曙光%潘祖飛%牛勇%賓萍%戴宇飛%鄭玉新
단화위%랭서광%반조비%우용%빈평%대우비%정옥신
职业暴露%焦炭%核质桥%核芽
職業暴露%焦炭%覈質橋%覈芽
직업폭로%초탄%핵질교%핵아
Occupational exposure%Coke%Nucleoplasmic bridge%Nuclear bud
目的 寻找评价多环芳烃致外周血淋巴细胞遗传物质损伤的新的效应性生物标志物.方法 采集158名焦炉工和69名非多环芳烃暴露者肘静脉血1 ml,应用外周血淋巴细胞胞质分裂阻滞微核法制备涂片,计数核质桥和核芽发生率,评价外周血淋巴细胞的染色体损伤水平;收集研究对象的职业史、年龄、性别、吸烟和饮酒等信息.结果 焦炉工外周血淋巴细胞核质桥率为(9.41±3.73)‰,高于对照组的(1.88±1.49)‰,差异有统计学意义(t=-16.2,P＜0.01);其核芽率为(7.13±4.01)‰,与对照组[(2.20 4-1.73)‰]相比差异有统计学意义(t=-9.79,P＜0.001).发现核质桥和核芽率与多环芳烃暴露等级具有剂量.效应关系.与男性焦炉工相比,女性焦炉工具有较少的核质桥[(7.14 4-3.04)‰比(9.63±3.73)‰,t=-2.62,P=0.01]和核芽[(4.71±2.73)‰比(7.36±4.05)‰,t=-2.33,P=0.02]发生率.未发现年龄、吸烟和饮酒等对焦炉工的核质桥和核芽率的影响.结论 多环芳烃可致暴露工人染色体损伤增加,核质桥和核芽可以作为焦炉工染色体损伤的效应性生物标志物.
目的 尋找評價多環芳烴緻外週血淋巴細胞遺傳物質損傷的新的效應性生物標誌物.方法 採集158名焦爐工和69名非多環芳烴暴露者肘靜脈血1 ml,應用外週血淋巴細胞胞質分裂阻滯微覈法製備塗片,計數覈質橋和覈芽髮生率,評價外週血淋巴細胞的染色體損傷水平;收集研究對象的職業史、年齡、性彆、吸煙和飲酒等信息.結果 焦爐工外週血淋巴細胞覈質橋率為(9.41±3.73)‰,高于對照組的(1.88±1.49)‰,差異有統計學意義(t=-16.2,P＜0.01);其覈芽率為(7.13±4.01)‰,與對照組[(2.20 4-1.73)‰]相比差異有統計學意義(t=-9.79,P＜0.001).髮現覈質橋和覈芽率與多環芳烴暴露等級具有劑量.效應關繫.與男性焦爐工相比,女性焦爐工具有較少的覈質橋[(7.14 4-3.04)‰比(9.63±3.73)‰,t=-2.62,P=0.01]和覈芽[(4.71±2.73)‰比(7.36±4.05)‰,t=-2.33,P=0.02]髮生率.未髮現年齡、吸煙和飲酒等對焦爐工的覈質橋和覈芽率的影響.結論 多環芳烴可緻暴露工人染色體損傷增加,覈質橋和覈芽可以作為焦爐工染色體損傷的效應性生物標誌物.
목적 심조평개다배방경치외주혈림파세포유전물질손상적신적효응성생물표지물.방법 채집158명초로공화69명비다배방경폭로자주정맥혈1 ml,응용외주혈림파세포포질분렬조체미핵법제비도편,계수핵질교화핵아발생솔,평개외주혈림파세포적염색체손상수평;수집연구대상적직업사、년령、성별、흡연화음주등신식.결과 초로공외주혈림파세포핵질교솔위(9.41±3.73)‰,고우대조조적(1.88±1.49)‰,차이유통계학의의(t=-16.2,P＜0.01);기핵아솔위(7.13±4.01)‰,여대조조[(2.20 4-1.73)‰]상비차이유통계학의의(t=-9.79,P＜0.001).발현핵질교화핵아솔여다배방경폭로등급구유제량.효응관계.여남성초로공상비,녀성초로공구유교소적핵질교[(7.14 4-3.04)‰비(9.63±3.73)‰,t=-2.62,P=0.01]화핵아[(4.71±2.73)‰비(7.36±4.05)‰,t=-2.33,P=0.02]발생솔.미발현년령、흡연화음주등대초로공적핵질교화핵아솔적영향.결론 다배방경가치폭로공인염색체손상증가,핵질교화핵아가이작위초로공염색체손상적효응성생물표지물.
Objective To seek new effect biomarkers as to evaluating the chromosomal damage in peripheral blood lymphocytes in coke-oven workers who were exposed to polycyclic aromatic hydrocarbons (PAHs).Methods One hundred and fifty-eisht coke.oven worker$and 69 controls were recruited in tlIis study.Nucleoplasmic bridges and nuclear buds were counted as indicators of chromosomal damage in terms of cytokinesis-block micronucleus(CBMN)tesL Occupational history,age,sex,smoking and alcohol using status of all subjects were coilected by questionnaire.Results Frequencies of nucleoplasmic bridge in coke. Oven worker$were(9.41±3.73)‰,and the frequencies of nuclear buds were(7.13±4.01)‰.which were significantly hisher(P<0.01)than those of controls(1.88±1.49)‰and(2.20±1.73)‰ respectively.The dose—effect relationships between nucleoplasmic bridges or nuclear buds and PAHs exposure levels were identifie&Compared with male coke.oven workelf8.female worker8 had lessnucleoplasmic bridges or nuclear buds.No effects of age,smoking and alcohol using were found on nucleoplasmic bridges or nuclear buds among coke—oven workers.Conclusion Nucleoplasmic bridges and nuclear buds might be effect biomarkers in coke.oven workers.
