CAJ | 학술논문

目的探讨Ras相关区域家族1A(RASSF1A)、半乳糖凝集素3(Galectin-3)和甲状腺过氧化物酶(TPO)mRNA在甲状腺乳头状癌组织及其他滤泡源性良性病变组织中的表达情况和临床意义.方法收集73例甲状腺肿块手术标本,其中甲状腺乳头状癌23例,结节性甲状腺肿16例,甲状腺腺瘤29例,桥本氏病5例.采用逆转录聚合酶链反应(RT-PCR),检测73例甲状腺组织标本中RASSF1A、Galectin-3和TPO mRNA的表达情况.结果 RASSF1A、Galectin-3和TPO mRNA在23例甲状腺乳头状癌组织中的阳性表达率分别为26.1%、82.6%和21.7%,均与其他滤泡源性良性病变中的表达率差异有统计学意义(均P<0.05);RASSF1A、Galectin-3和TPO mRNA在各滤泡源性良性病变中的表达率差异均无统计学意义(均P>0.05).在甲状腺滤泡源性良恶性病变中,RASSF1AmRNA与Galectin-3 mRNA的表达呈负相关(Pearson点相关系数=-0.4090,χ2=12.2088,P=0.000);RASSFIA mRNA与TPO mRNA的表达旱正相关(Pearson点相关系数=0.2763,χ2=4.8217,P=0.028).结论 RASSF1A和TPO mRNA在甲状腺乳头状癌组织中的表达普遍缺失,而Galectin-3mRNA表达率较高.RASSF1A、Galectin-3和TPO mRNA与甲状腺乳头状癌的发生和发展密切相关,可作为诊断和鉴别诊断甲状腺乳头状癌的标志物,且联合检测的结果更可靠.
목적 탐토Ras상관구역가족1A(RASSF1A)、반유당응집소3(Galectin-3)화갑상선과양화물매(TPO)mRNA재갑상선유두상암조직급기타려포원성량성병변조직중적표체정황화림상의의.방법 수집73례갑상선종괴수술표본,기중갑상선유두상암23례,결절성갑상선종16례,갑상선선류29례,교본씨병5례.채용역전록취합매련반응(RT-PCR),검측73례갑상선조직표본중RASSF1A、Galectin-3화TPO mRNA적표체정황.결과 RASSF1A、Galectin-3화TPO mRNA재23례갑상선유두상암조직중적양성표체솔분별위26.1%、82.6%화21.7%,균여기타려포원성량성병변중적표체솔차이유통계학의의(균P<0.05);RASSF1A、Galectin-3화TPO mRNA재각려포원성량성병변중적표체솔차이균무통계학의의(균P>0.05).재갑상선려포원성량악성병변중,RASSF1AmRNA여Galectin-3 mRNA적표체정부상관(Pearson점상관계수=-0.4090,χ2=12.2088,P=0.000);RASSFIA mRNA여TPO mRNA적표체한정상관(Pearson점상관계수=0.2763,χ2=4.8217,P=0.028).결론 RASSF1A화TPO mRNA재갑상선유두상암조직중적표체보편결실,이Galectin-3mRNA표체솔교고.RASSF1A、Galectin-3화TPO mRNA여갑상선유두상암적발생화발전밀절상관,가작위진단화감별진단갑상선유두상암적표지물,차연합검측적결과 경가고.
Objective To investigate the mRNA expressions of RASSF1A, Galectin-3 and TPO in papillary thyroid carcinoma and some other thyroid benign lesions, and evaluate their diagnostic significance. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of RASSF1 A, galectin-3 and TPO in the samples from 73 cases, including 23 cases with papillary thyroid cancer, 16 with nodular gaiter, 29 with thyroid adenoma and 5 with Hashimoto's disease. Results A statistically significant difference in the mRNA expression of RASSF1 A, Galectin-3 and TPO was observed between papillary thyroid carcinoma and follicular benign lesions (P < 0.05). However, there was no significant difference among various kinds of benign lesions (P > 0.05). A negative correlation of the expression of RASSF1A and Galectin-3 mRNA was found between thyroid benign lesions and malignant ones (P = 0.000). While the mRNA expression of RASSFI A and TPO was positively correlated between benign and malignant lesions (P = 0.028). Conclusion Loss of expression of RASSF1 A and TPO mRNA but high expression of Galeetin-3 mRNA in papillary thyroid carcinoma are common. Therefore, the products of these three genes may be closely related to the development of thyroid papillary carcinoma, and may be used as useful markers in differential diagnosis of papillary thyroid carcinoma from the benign lesions. The results are more reliable if this detection method is used in combination with other techniques.